Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB122 44,301 (T)9→8 intergenic (+132/‑359) oprD_1 → / → czcR_2 Porin D/Transcriptional activator protein CzcR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB12244,2930T.88.3% 202.2 / 29.6 62intergenic (+124/‑367)oprD_1/czcR_2Porin D/Transcriptional activator protein CzcR
Reads supporting (aligned to +/- strand):  ref base T (5/2);  new base . (22/31);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCTGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCGG  >  ACB122/44162‑44412
                                                                                                                                   |                                                                                                                       
ttCTGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGGCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCttt                                                                                                                       <  3:344664/134‑1 (MQ=255)
   tGTAATTCGCTAACTGGGACAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACATTGAGTGTCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTTTCGCCCATTGGGCGCCtttttttt                                                                                                                  >  8:268743/1‑136 (MQ=255)
      aaTTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCttttttttgc                                                                                                                >  4:25844/1‑133 (MQ=255)
                     ccAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAg                                                                                                <  6:363468/135‑1 (MQ=255)
                       aGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCG‑CGTTTTTTTTGCCTAAAATTCAAGTAGGc                                                                                              >  4:29922/1‑135 (MQ=255)
                       aGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCACTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGc                                                                                              <  6:39560/135‑1 (MQ=255)
                            tCCATTGGCGGGGACTCTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGATTAAGTGCTCCTTTGGAAAAAGGTGGATATATGGCGCCCATTGGGCGCC‑TTTTTTTAGCCTAAAATTCAAGTAGGCGTTCg                                                                                         <  7:219831/135‑1 (MQ=255)
                               aTTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGAtt                                                                                      <  4:175767/135‑1 (MQ=255)
                                   gCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcg                                                                                 >  8:219210/1‑136 (MQ=255)
                                     gggTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTAAAGTAGGCGTTCGATTGTAcgca                                                                               >  8:253349/1‑136 (MQ=255)
                                      ggTCCTGTTAGTAAAACGAGCATTACGGATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcgca                                                                               <  5:237484/135‑1 (MQ=255)
                                      ggTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcgca                                                                               >  5:152747/1‑135 (MQ=255)
                                         ccTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTGTTTTTTTGCTTATAA‑TCAAGTAGGCGTGCGCTTGTTcgcaaagc                                                                           >  7:391590/1‑132 (MQ=255)
                                          cTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGGCTAAAATTAAAGTTGTCGTTGGATTGTAagcatccg                                                                           >  3:368825/1‑133 (MQ=255)
                                           tGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATTTTTGGCGCCCATTGGGCGCCTTTTTTTTTGCCTAAAA‑TAAATTAGGCGTTAGATTTTTcgcaacgcc                                                                          >  6:48632/1‑134 (MQ=255)
                                           tGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAg                                                                                                   <  4:105813/110‑1 (MQ=255)
                                           tGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAg                                                                                                   >  3:105813/1‑110 (MQ=255)
                                           tGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcgcatcgcat                                                                         >  7:106757/1‑136 (MQ=255)
                                                gTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTT‑CCTAAAAATCAAGTAGTCGTTCGATTGTACGCATAGGATGCCg                                                                     >  5:340040/1‑135 (MQ=255)
                                                gTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                     <  8:326794/135‑1 (MQ=255)
                                                     aCGAGCTTTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAATAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGaa                                                                <  3:176484/135‑1 (MQ=255)
                                                     aCGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGaa                                                                <  2:87186/135‑1 (MQ=255)
                                                     aCGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAAGCATCGCCTGCCGGTGaa                                                                >  3:46487/1‑135 (MQ=255)
                                                        aGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGtt                                                             <  1:152327/135‑1 (MQ=255)
                                                          cATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCa                                                           <  5:358831/135‑1 (MQ=255)
                                                            ttACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGa                                                        <  8:87259/136‑1 (MQ=255)
                                                             tACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGa                                                        <  1:278723/135‑1 (MQ=255)
                                                             tACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGAGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGa                                                        <  6:252131/135‑1 (MQ=255)
                                                                          tGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑GTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGGAGTTCAAGACGACACCAATGccc                                          >  7:127368/1‑136 (MQ=255)
                                                                            aGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAcc                                          <  6:206282/134‑1 (MQ=255)
                                                                            aGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAc                                           <  5:382430/133‑1 (MQ=255)
                                                                            aGTGGCTTAAGTGCTCATTTGGTAAAAGGTGGATATTTTGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGTCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAcc                                          <  4:92783/134‑1 (MQ=255)
                                                                             gTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTTAAATTAAATTATGCGTTGGATTGTTCGCATACCATGCCGGTGAAGTTAATGACTACACCAATGAcccc                                        >  4:31913/1‑133 (MQ=255)
                                                                             gTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑GTTTTTTTGCCTAAAATTAAAGTTGGCGTTCGATTTTACGCCTCGCATTCCGGTGAAGTTCATGACTACCCCAATGACCac                                        >  4:38093/1‑134 (MQ=255)
                                                                              gggCTTAAGTGCGCCTTGGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGt                                       <  5:207732/134‑1 (MQ=255)
                                                                               ggCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCGGTTTTTTTG‑CTAAAATTCAATAAGGGGGTGGATTGTACGCATCGCCTGCCCGTGAAGGTCATGACTACCCCAATTGCCAGtc                                      >  1:329196/1‑135 (MQ=255)
                                                                               ggCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGGCTAAAATTCAAGTAGGCGTTCGTTTGTTAGCAACGCATGCCGGTGAACTTCATTACTTCACCAATGACCAGta                                      >  6:98571/1‑134 (MQ=255)
                                                                               ggCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGtct                                     <  8:311128/136‑1 (MQ=255)
                                                                               ggCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑GTTTTTTTGCCTAAAAATCAACTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAAAGACCAggc                                      >  3:401578/1‑133 (MQ=255)
                                                                                 cTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTT‑CCTAAAATTAAAGTAGGAGTTCGATTGTACTCATCGCATGCCGGTGAAGTTGATTACTACACCAAAGACCAGGctct                                   >  7:191043/1‑136 (MQ=255)
                                                                                    aaGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTAAAGTAGGCGTTCGATTGTTCGCCTCGCATGCCGGTGAAGTTCATTACTACACCAATGACCAGTCTCtt                                  >  1:23838/1‑134 (MQ=255)
                                                                                     aGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGTGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTc                                 <  4:421630/134‑1 (MQ=255)
                                                                                        gCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAAtgt                            >  8:297821/1‑136 (MQ=255)
                                                                                         cTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTT‑CCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAAtgt                            <  8:27289/135‑1 (MQ=255)
                                                                                         cTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTT‑CCTAAAATTCAAGTAGGCGGTCGATTGTACGCATCGCATGACGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAAtgt                            >  5:257442/1‑135 (MQ=255)
                                                                                         cTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAAtgt                            <  7:219210/135‑1 (MQ=255)
                                                                                          tCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAAtgtg                           <  6:152747/135‑1 (MQ=255)
                                                                                             tttGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAg                         >  3:384039/1‑134 (MQ=255)
                                                                                             tttGGTAAAAGGTGGATATTAGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAAGTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGGGACGTTAATGCCTACACCAATGACCAGGCGCTTCAATGTgcgcc                       >  7:433371/1‑135 (MQ=255)
                                                                                               tGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGAC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGtt                                                             <  1:88240/96‑1 (MQ=255)
                                                                                               tGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGAC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGtt                                                             >  2:88240/1‑96 (MQ=255)
                                                                                                  tAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCt                   >  1:237295/1‑135 (MQ=255)
                                                                                                  tAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCt                   >  4:345768/1‑135 (MQ=255)
                                                                                                  tAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTc                  <  8:88205/136‑1 (MQ=255)
                                                                                                         tGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTc                                 >  2:261108/1‑114 (MQ=255)
                                                                                                         tGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTc                                 <  1:261108/114‑1 (MQ=255)
                                                                                                         tGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTAAACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTc            >  8:276214/1‑135 (MQ=255)
                                                                                                             tatTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTg       <  8:103690/136‑1 (MQ=255)
                                                                                                             tatTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTAGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCATTGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCt        <  2:158280/135‑1 (MQ=255)
                                                                                                                   gcgcCCATTGGGCGCCTTTTTTTTTGCCTAAAATTCATGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGcc   <  7:233824/135‑2 (MQ=255)
                                                                                                                   gcgcCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCgg  <  8:106757/135‑1 (MQ=255)
                                                                                                                    cgcCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCgg  <  4:384039/134‑1 (MQ=255)
                                                                                                                    cgcCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCgg  <  4:275564/134‑1 (MQ=255)
                                                                                                                         aTTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                     <  5:337612/62‑1 (MQ=255)
                                                                                                                         aTTGGGCGCC‑GTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                     >  6:337612/1‑62 (MQ=255)
                                                                                                                                   |                                                                                                                       
TTCTGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCGG  >  ACB122/44162‑44412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑