Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,157,9830AC44.9% 33.9 / 11.5 49S162A (TCG→GCG) hexR_1HTH‑type transcriptional regulator HexR
Reads supporting (aligned to +/- strand):  ref base A (8/19);  new base C (21/1);  total (29/20)
Fisher's exact test for biased strand distribution p-value = 1.85e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTTTTCACGTGCCAGGCGCGCCACTTCGACCAGTTCGCGGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACGGCGCGGCTGACCTGCT  >  ACB122/1157852‑1158108
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gTTTTCACGTGCCCGGCGCGCCACTTCGACCAGTTCGCGGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGacac                                                                                                                             <  3:197739/135‑1 (MQ=255)
              ggCGCGCCACTTCGACCAGTTCGCGGGTGCGCCCGGTGTAGGAAATGATCACGAACAGTTCGCCGGTGGGGGCGACGGAGGCCAGCATGCGCTGCATCAGCACATCGGGGTGGGCCGACACGGCCAGGTTGAAGc                                                                                                               <  4:410457/135‑1 (MQ=255)
               gcgcgcCACTTCGACCAGTTCGCGGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTGAAGCg                                                                                                              >  1:25881/1‑135 (MQ=255)
                cgcgcCACTTCTACCAGTTCGCGGGGCCGCCCGGGGTAGGAATTGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTGAAGc                                                                                                               <  1:327431/133‑1 (MQ=255)
                   gcCACTTCGACCCGTGCGCGGGGGCGCCCGGTGTAGCAAATCAGCACGACCAGAGCGCCGGTGGGGGCGCCCGAGGCCAGCAGGCGCTGCATCAGCACAGCGGCGTGGGCCGACACGGCCAGGTTGAAGCGgaag                                                                                                          <  4:57473/135‑1 (MQ=255)
                       cTTCGACCAGTTCGGGGGTGCGCCGGGTTTAGGAATTGATCACGAACTGATGGCGGGTGGGGGCGACCGAGGCCAGCATGCGCTGCATCTGCACAGCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACt                                                                                                      <  6:154993/135‑1 (MQ=255)
                       cTTCGACCAGTTCGCGGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGCCACGGCCAGGTTGAAGCGGCAGAACt                                                                                                      >  2:348782/1‑135 (MQ=255)
                           gACCAGTTCGCGGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCAGGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTtgtg                                                                                                  <  8:13844/135‑1 (MQ=255)
                                    gcgGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGCCCCGGCCCGGTTGCAGCGGAAGAACTTGTGCTGCGCATc                                                                                         >  2:98364/1‑135 (MQ=255)
                                    gcgGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACCCGGCCAGGGTGCAGCGGAAGAACTTGTGCTGCGCCTcc                                                                                        >  7:200939/1‑136 (MQ=255)
                                     cgtgTGGGCCGGGTGTAGGAATTTTTCAAGAACTGTGCGCCGGTGTGGGCTACCGAGGCCAGATGGCTCTGAATCTGCACATCGGCGTGGCCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATcc                                                                                        <  6:390/132‑1 (MQ=255)
                                     cgGGTGCGCCCGGTGTAGGAAATGATCACGAACAGAGCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATcc                                                                                        <  3:430907/135‑1 (MQ=255)
                                           cgcCGGGTGTAGCACATGAGCACGACCCGATCGCCGGGGGGGGGCACCGTGCCCTGCATGCGCTGCATCAGCACAGCGGCGGGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGcc                                                                                 <  1:312296/135‑1 (MQ=255)
                                             cccGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGCGCCGCCCCCGCCAGCGTGTAGCGCTCGCGCAAGTGCTGCGCCACCA‑GGGCCAcc                                                                              >  7:402127/1‑136 (MQ=255)
                                              ccGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGGCGCCACGGCCAGGTTGCAGCGGAAGAACTTGTGCTGCGCCTCCA‑GGGCcccc                                                                              >  3:411721/1‑135 (MQ=255)
                                              ccGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGCCACGGCCAGGTTGCAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCAcc                                                                              >  2:14035/1‑135 (MQ=255)
                                              ccGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACCCGGCCAGGTTGAAGCGGAAGAACGTGTGCTGCGCATCCA‑GGGCcccc                                                                              >  5:391679/1‑135 (MQ=255)
                                              ccGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTGCAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCCCCg                                                                             >  8:53462/1‑136 (MQ=255)
                                              ccGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCCCCCCCGCGAGGTTGAAGGGGAATAACGTTTTGGGCGCATCCA‑GGGCcccc                                                                              >  5:31649/1‑135 (MQ=255)
                                                 gtgtAGTAAATGATCACGACCCGAGCGCCGGTGGGGGCGACCGAGGCCAGCAGGCGCTGCATCAGCACAGCGGCGGGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCACCggg                                                                           <  2:295998/135‑1 (MQ=255)
                                                          aTGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGCCACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCACCCGGGCTGAGgcg                                                                  >  5:403595/1‑135 (MQ=255)
                                                               cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGGCCCCCCCGCCCGGTTGGAGCGGGAGCACTTGTGGTGCGCCTCCA‑CGGCCCCCGGGGCGGCGGCGccccg                                                             >  6:270791/1‑132 (MQ=255)
                                                               cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGCCCCCGGCACGGTGAATCGGGACAACATGTGCTGGGCCTCCA‑GGGCCCCCCGGGCGGAGGCGCCGac                                                             >  5:20797/1‑134 (MQ=255)
                                                               cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGCCACGGCCAGGTTGAAGCGGCAGAACTTGTGCTGCGCATCCA‑GGGCCACCGGGGCTGCGGCGCCGCgg                                                            >  8:33299/1‑136 (MQ=255)
                                                               cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGCCACGGCCAGGTTGAAGCGGAATAACTTGTGCTGCGCATCCA‑GGGCCACCGGGGCTGAGGCGCCGAg                                                             >  2:139860/1‑135 (MQ=255)
                                                               cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGCCACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCCCCGGGGCTGAGGCGCCGAg                                                             >  5:381580/1‑135 (MQ=255)
                                                               cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGCCACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCCCCGGGGCTGAGGCGCCGAg                                                             >  2:109674/1‑135 (MQ=255)
                                                               cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGCCACGGCCAGGTTGAAGCGGAAGAACATTTGCTGCGCATCCC‑GGGCCACCGGGGCGGCGGCGccccg                                                             >  3:19389/1‑132 (MQ=255)
                                                               cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGCCACCGCCAGGGTGAAGCGGAAGAACTTGTGCAGCGCATCCA‑CGGCCACCGGGGCAGACGCGCCGAgg                                                            >  8:287351/1‑136 (MQ=255)
                                                               cACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTGTAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCACAGGGGGTGAGGCGCCgcg                                                             >  6:6755/1‑133 (MQ=255)
                                                                ccGAACTGATCGCGGGGGTGGGCGACCGAGGCCAGCTTGCGATGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCACCGGGGCTGAGGCGCCGAgg                                                            <  6:391679/134‑1 (MQ=255)
                                                                      aGATCGCCGGGGGGGGCGACCGAGGCCAGCATGCGCGTCAGCAGCACAGCGGCGGGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCACCGGGGCTGAGGCGCCGAGGCCgaag                                                      <  5:207979/135‑1 (MQ=255)
                                                                         gcGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGt                                                  <  8:265892/135‑1 (MQ=255)
                                                                          cGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGCCACGGCCAGGTTGAAGCGGAAGCACTTGTGCTGCGGCTCCC‑GGGCCCCCGGGGCTGCGGCGCCGCGGCCGCAGAAGt                                                  >  1:329388/1‑135 (MQ=255)
                                                                               gtgtGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGGGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATc                                             <  5:149772/135‑1 (MQ=255)
                                                                               gtggGGGCGACCGAGGCCCGCATGGGCGGCATCAGCACATCGGGGGGGCCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATc                                             <  6:81684/135‑1 (MQ=255)
                                                                                   gggCGAGCGAGGACAGGATCCGCTGCATCAGCCGCGCGGCGGGGGCCGAGACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCg                                        <  7:419823/136‑1 (MQ=255)
                                                                                   gggCGACCGAGGCCCGCCTGCGCGGCATCAGCACAGCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCg                                        <  8:220166/136‑1 (MQ=255)
                                                                                        acggAGTCCAGCTGGCGATGCAGAATCACATCGGCGTGGGCCTACACTGCCAGGTTGAAGCGGCATAACTTGTGCTGAGCATCCA‑GGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGcc                                    <  6:318374/132‑1 (MQ=255)
                                                                                             ggCCAGCAGGCGCGGCATCAGCACAGCGGCGGGGGCCGACACGGGCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGat                               <  6:303946/135‑1 (MQ=255)
                                                                                               ccAGCATGCGCTGCATCAGCACATCGGCGTGGGGCGCCACCGCCCGGGTGGAGCGGGAGAACTTGTTCTGGGCCTCCACGGGCCCCCGGGG‑GGGGGGGCCCGCGCCGCACAAGTGGGTGTGCCGGGCCAGGatga                             >  2:441524/1‑133 (MQ=255)
                                                                                                 aGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGGTGAAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGatcatc                           >  4:383390/1‑135 (MQ=255)
                                                                                                      gcgcTGCATCAGCACATCGGCGTGGGCCGCCACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCCTCCA‑GGGCCCCCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGCTCTGCCGGGCCTGGATCATCATGTc                      >  3:391148/1‑135 (MQ=255)
                                                                                                          tGCATCAGCACATCGGCGTGGGCCGCCACGGCCAGGTTGCAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCCCCGGGGCTGAGGCGCCGCGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACg                  >  2:154772/1‑135 (MQ=255)
                                                                                                               cAGCACATCGGCGTGGGCCGCCACGGCCAGGGTGAAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCACCGGGGCGGAGGCGCCGCGGCCGAAGAAGTGGATCTGCCGGGCCGGGCTCATCATGTCCACGGcgcg             >  5:211830/1‑135 (MQ=255)
                                                                                                                 gCACAGCGGCGGGGCCCGCCACGGCCAGGTTGACGCGGAAGAACTTGGGCTGCGCATCCA‑GGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACGGCGCGGCt          <  7:33299/136‑1 (MQ=255)
                                                                                                                       cGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACGGCGCGGCTGACct     <  3:383390/135‑1 (MQ=255)
                                                                                                                         gCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCACCGGGGCTGCGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACCGCGCGGCTGACcggc   >  5:356819/1‑135 (MQ=255)
                                                                                                                         gCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACGGCGCGGCTGACctgct  >  7:9489/1‑136 (MQ=255)
                                                                                                                          cGTGGGCCGCCCCGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACGGCGCGGCTGCCctgcg  >  3:437397/1‑134 (MQ=255)
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GTTTTCACGTGCCAGGCGCGCCACTTCGACCAGTTCGCGGGTGCGCCCGGTGTAGGAAATGATCACGAACAGATCGCCGGTGTGGGCGACCGAGGCCAGCATGCGCTGCATCAGCACATCGGCGTGGGCCGACACGGCCAGGTTGAAGCGGAAGAACTTGTGCTGCGCATCCA‑GGGCCACCGGGGCTGAGGCGCCGAGGCCGAAGAAGTGGATCTGCCGGGCCTGGATCATCATGTCCACGGCGCGGCTGACCTGCT  >  ACB122/1157852‑1158108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑