Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,482,0140TG34.7% 59.6 / 10.3 49V173G (GTG→GGG) CJ019_01397GTP cyclohydrolase 1 type 2
Reads supporting (aligned to +/- strand):  ref base T (19/13);  new base G (1/16);  total (20/29)
Fisher's exact test for biased strand distribution p-value = 2.40e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAACCCCAAAGTGGTCGGGCTGATTGGTTCGCTCGCCGAATCCATGACCGCGCGCGATTTTGCCCGCCGGGTGCAACAGGTGCTGGGGCGTGAGCCGTTGCTGGTCGAAGGCAATGAAATGATCCGCCGGGTGGGCTGGTGCACCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCTGACCGGCGAGGCGTCCGAGCAGACCTACCACAGTGCGCGCGAGAATGGCGTCAGCTTCATTG  >  ACB122/1481883‑1482145
                                                                                                                                   |                                                                                                                                   
aaaCCCCAAAGTGGTCGGGCTGATTGGTTCGCTCGCCGAATCCATGACCGCGCGCGATTTTGCCCGCCGGGTGCAACAGGTGCTGGGGCGTGAGCCGTTGCTGGTCGAAGGCAATGAAATGATCCGCCGGGTggg                                                                                                                                  >  6:12276/1‑135 (MQ=255)
 aaCCCCAAAGTGGTCGGGCTGATTGGTTCGCTCGCCGAATCCATGACCGCGCGCGATTTTGCCCGCCGGGTGCAACAGGTGCTGGGGCGTGAGCCGTTGCTGGTCGAAGGCAATGAAATGATCCGCCGGGTGGGc                                                                                                                                 >  3:403657/1‑135 (MQ=255)
           ggggcGGGCTGATTGGTTCGCGCGCCGAAGCCATGACCGCGCGCGATTGTGCCCGCCGGGTGCAACAGGTGCTGGGGCGTGAGCCGTTGCGGGGCGACGGCAATGAAATGATCCGCCGGGGGGGCTGGTGCACcg                                                                                                                       <  5:216325/131‑1 (MQ=255)
                gggCTGATTGGTTCGCTCGCCGAATCCATGACCGCGCGCGATTTTGCCCGCCGGGTGCAACAGGTGCTGGGGCGTGAGCCGTTGCTGGTCGAAGGCAATGAAATGATCCGCCGGGTGGGCTGGTGCACcggcggc                                                                                                                  >  2:164855/1‑135 (MQ=255)
                gggCTGATTGGTTCGCTCGCCGAATCCATGACCGCGCGCGATTTTGCCCGCCGGGTGCAACAGGTGCTGGGGCGTGAGCCGTTGCTGGTCGAAGGCAATGAAATGATCCGCCGGGTGGGCTGGTGCACcggcggc                                                                                                                  >  3:356005/1‑135 (MQ=255)
                gggCTGAGTGGTGCGCTCGCCGAATCCATGCCCGCGCGCGATTTTGCCCGCCGGGGGCAACAGGGGCGGGGGCGTGCGCCGTTGCTGGGCGAAGGCAATGAAATGATCCGCCGGGTGGGCGGGTGCACcggcggc                                                                                                                  <  6:126628/135‑1 (MQ=255)
                 ggCTGATTGGTTCGCTCGCCGAATCCATGACCGCGCGCGATTTTGCCCGCCGGGTGCAACAGGTGCTGGGGCGTGCGCCGGTGGGGGGCGAACGCAAAGAAATTATCCCCCCGGGGGGCTGGGGGCCccgcggcc                                                                                                                 >  1:218958/1‑134 (MQ=255)
                 ggCTGATTGGTTCGCTCGCCGAATCCATGACCGCGCGCGATTTTGCCCGCCGGGTGCAACAGGTGCTGGGGCGTGAGCCGTTGCTGGTCGAAGGCAATGAAATGATCCGCCGGGTGGGCTGGTGCACcggcggcgg                                                                                                                >  7:377372/1‑136 (MQ=255)
                 ggCTGATTGGTTCGCTCGCCGAATCCATGACCGCGCGCGATTTTGCCCGCCGGGTGCAACAGGTGCTGGGGCGTGAGCCGTTGCTGGTCGAAGGCAATGAAATGATCCGCCGGGTGGGCTGGTGCACcggcggcg                                                                                                                 >  3:28999/1‑135 (MQ=255)
                       ttGGTGCGCTCGCCGAATCCATGACCGCGCGCGATTTTGCCCGCCGGGTGCAACAGGTGCTGGGGCGTGAGCCGTGGCTGGTCGAAGGCAATGAAATGAGCCGCCGGGGGGGCTGGTGCACCGGCGGCGGGCa                                                                                                             <  3:175043/133‑1 (MQ=255)
                                   ccGAATCCATGAGCGCGCGCGATTTTGCCCGCCGGGTGCAACAGGTGCTGGGGCGTGAGCCGTTGCTGGTCGAAGGCAATGAAATGATCCGCCCGGTGGGCTGGTGCACCGGCGGGGGGCACGGCGATATCGaca                                                                                               >  3:57521/1‑135 (MQ=255)
                                   ccGAATCCATGACCGCGCGCGATTTTGCCCGCCGGGTGCAACAGGTGCTGGGGCGTGAGCCGTTGCTGGTCGAAGGCAATGAAATGATCCGCCGGGTGGGCTGGTGCACcggcggc                                                                                                                  >  6:198628/1‑116 (MQ=255)
                                   ccGAATCCATGACCGCGCGCGATTTTGCCCGCCGGGTGCAACAGGTGCTGGGGCGTGAGCCGTTGCTGGTCGAAGGCAATGAAATGATCCGCCGGGTGGGCTGGTGCACcggcggc                                                                                                                  <  5:198628/116‑1 (MQ=255)
                                   ccGAATCCATGACCGCGCGCGATTTTGCCCGCCGGGTGCAACAGGTGCTGGGGCGTGAGCCGTTGCTGGTCGAAGGCAATGAAATGATCCGCCGGGTGGGCTGGTGCACCGGCGGCGGGCAGGGCTATATCGaca                                                                                               >  6:208590/1‑135 (MQ=255)
                                                  cgctctaTTTTGCCCTCGGGTTGAAACAGGTGCTTTGGCGTGAGCCTTTGCGTGTCTAAGGAAATGAAATCAGCCGCCGGGTGGGCTTTTGCACCGGCTGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTgg                                                                               <  8:76019/130‑1 (MQ=255)
                                                  cgcgcgATTTGCCCCGCGGGGTGAAACAGGGGCGGGGGCGGGCGCGGTGGCGGGTCGACGGCAATGAAATGATCCGCCGGGGGGGCGGGGCCACGGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTgg                                                                               <  7:10288/136‑1 (MQ=255)
                                                   gggggtttttGCCCGCGGGGTGCAACTGGGGGTGGGGCGTGTGCGGTTGCTGGGCGAAGGCAATCAAAGGATCCGCCGGGGGGGCTGGTGCACCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTgg                                                                               <  1:247173/129‑1 (MQ=255)
                                                   gcgcgATTTTGCCCGCCGGGTGCAACAGGTGCTGGGGCGTGAGCCGTTGCTGGTCGAAGGCAATGAAATGATCCGCCGGGTGGGCTGGTGCACCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTgg                                                                               >  4:325248/1‑135 (MQ=255)
                                                     gcgATTTTGCCCGCCGGGTGCAACAGGTGCTGGGGCGTGAGCCGTTGCTGGTCGAAGGCAATGAAATGATCCGCCGGGTGGGCTGGTGCACCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGt                                                                            >  8:33153/1‑136 (MQ=255)
                                                     gcgATTTTGCCCGCCGGGTGCAACAGGTGCTGGGGCGAGAGCCGTTGCTGGTCTAAGGCAATGAAATGATCCGCCGGGTGGGCTGGTGCACCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGt                                                                            >  8:330344/1‑136 (MQ=255)
                                                       gtttttGCCCGCGTGGTGAAACTGGTGGTTGGGCGTGCGCCTGTGCTGGTGGAAGGAAATGAATTTATCCGCCGGGTGGGCTGGTGAACCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGTGTc                                                                           <  6:417812/133‑1 (MQ=255)
                                                       gttttcGCCCGCCGGTCGCAACTGGTGCGGGGGCGTTTGGGGTGGGGGTGCTATGCCATTGAATTGAGCCGCCGGGGGGGCTGGGGCCCCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTc                                                                           <  4:330841/133‑1 (MQ=255)
                                                       gATTTTGCCCGCCGGGTGCAACAGGTGCTGGGGCGTGAGCCGTTGCTGGGCGAAGGCAATGAAATGATCCGCCGGGTGGGCTGGTGCACCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTc                                                                           <  3:431348/135‑1 (MQ=255)
                                                       gATTTGGCCCGCCGGGTGCAACAGGTGCTGGGGGGTGAGCGGTTGCTGGTCGAAGGCAATGAAATGAGCCGCGGGGTGGGCTGGTGCACCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTc                                                                           <  4:403657/135‑1 (MQ=255)
                                                                        gCAACTGGTGCTGGGGCGGGAGCCGTGGCTGGTCGACGGAAATTAAATGATCCGCCGGGGGGGCTGGGGCACCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCTGACCgg                                                          <  6:116771/135‑1 (MQ=255)
                                                                             aGGGGCGGGGGCGTGCGCCGTTGCGGGTCGACGGCAATGAAATGATCCGCCGGGGGGGCGGGGGCCCCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCTGACCGGCGAgg                                                     <  3:90070/135‑1 (MQ=255)
                                                                             aGGGGCGGGGGCGGGAGCGGTGGCTGGTCGACGGCAATTAAAGGATCCGCCGGGGGGGCTGGTGCACCGGGGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCAGACCGGCGAgg                                                     <  3:323463/135‑1 (MQ=255)
                                                                             aGGGGCGGGGGCGGGAGCCGTGGCGGGTCGCAGCCAAGCAAATGATCCGCCGGGGGGGCTGGGGCACCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCTGACCGGCGAgg                                                     <  6:74635/135‑1 (MQ=255)
                                                                                  cgggggCGGGCGCCGTTGCGGGGCCACGGCAATGAAAGGCGCCGCCGGGGGGGCGGGTGCCCCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCTGACCGGCGAGGCGTCCg                                               <  7:223614/134‑1 (MQ=255)
                                                                                  cTGGGGCGTGAGCCGTTGCGGGTCGAAGGCAATGAAATGATCCGCCGGGTGGGCTGGTGCACCGGCGGCGGGCAGGGCTATATCGACCCGGCGATTGCCGCTGGGGTCGCCCTGGACCTGCCCGGCGAGGCGTcc                                                >  5:316464/1‑135 (MQ=255)
                                                                                  cTGGGGCGTGAGCCGGTGCTGGGCGAAGCCAATGAAATGCGCCGCCGGGGGGGCTGGTGCACCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCTGACCGGCGAGGCGTCCg                                               <  8:272456/136‑1 (MQ=255)
                                                                                  cTGGGGCGGGAGCCGTGGCTGGTCGAAGGCAATGAAATGAGCCGCCGGGTGGGCTGGCGCACCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCTGACCGGCGAGGCGTCCg                                               <  8:150817/136‑1 (MQ=255)
                                                                                   tGGGGGGGGCGCGGTGGCGGGGCGAAGGAATTTAAAGGACCGCCCGGGGGGGGGGGTGCACGGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCTGACCGGCGAGGCGTCCg                                               <  2:440929/135‑1 (MQ=255)
                                                                                   tGGGGCGTGCGCCGTTGCTGGTCGCAGGCAAGTAAATGACCCGCCGGGTGGGCTGGTGCACCGGGGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCTGACCGGCGAGGCGTCCg                                               <  4:315518/135‑1 (MQ=255)
                                                                                   gggggCGTGAGCCGTTGCTGGGCGAAGGCAAGGAAATGATCCGCGGGGGGGGCTGGTGCACGGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCTGACCGGCGAGGCGTCCg                                               <  4:403580/134‑1 (MQ=255)
                                                                                   gggggCGGGCGCCGTGGCGGGCCGACCCCAATCAAATCAGCCGCCGGGGGGGCTGGTCCCCCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCTGACCGGCGAGGCGTCCg                                               <  5:89022/134‑1 (MQ=255)
                                                                                    ggggCGTGAGCCGTTGCTGGTCGAAGGCAATGAAATGATCCGCCGGGTGGGCGGGTGCACCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCTGACCGGCGAGGCGTCCGAg                                             <  8:275853/136‑1 (MQ=255)
                                                                                    ggggCGGGAGCCGGTGCTGGTCGAAGGCAATGAAATGAGCCGCCGGGGGGGCGGGTGCACCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGGCGACCTGTACCTGACCGGCGAGGCGTCCGAg                                             <  7:332822/136‑1 (MQ=255)
                                                                                     gggCGTGAGCCGGTGCTGGTTGACGGAATTCAAATGATCGGCCGGGTGGGGTGGTGCTCGGGCGGGGGGCAGGGCTATTTCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCTGACCGGCGAGGCGTCCGAg                                             <  1:153207/135‑1 (MQ=255)
                                                                                     gggCGTGAGCCGGTGCGGGTCGCAGGCAATCAACTCCTCCGCCGGGTGGGCTGGTGCACCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCTGCCCGGCGAGGCGTCCGAg                                             <  2:21369/135‑1 (MQ=255)
                                                                                       gCGTGAGCCGTTGCTGGTCTAAGGCAATGAAATGATCCGCCGGGTGGGCTGGTGCACCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCTGACCGGCGAGGCGTCCGa                                              >  5:379107/1‑132 (MQ=255)
                                                                                       gCGTGAGCCGTTGCTGGTCGAAGGCAATGAAATGATCCGCCGGGTGGGCTGGTGCACCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCTGACCGGCGAGGCGTCCGa                                              >  1:18032/1‑132 (MQ=255)
                                                                                             gCCGTTGCTGGTCGAAGGCAATGAAATGATCCGCCGGGTGGGCTGGTGCACCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCTGACCGGCGAGGCGTCCGAGCAGACCTa                                     >  2:248702/1‑135 (MQ=255)
                                                                                             gCCGTTGCTGGTCGAAGGCAATGAAATGATCCGCCGGGTGGGCTGGTGCACCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCTGACCGGCGAGGCGTCCGAGCAGACCTAc                                    >  7:150817/1‑136 (MQ=255)
                                                                                               cGTGGCTGGGCGACGGAAATCAAATGATCCGCCGGGGGGGCTGGTGCACCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCTGACCGGCGAGGCGTCCGAGCAGACCTAcc                                   <  5:179008/135‑1 (MQ=255)
                                                                                                    cTGGTCGAAGGCAATGAAATGATCCGCCGGGTGGGCTGGTGCACCGGCGGCGGGCAGGGCTATATCGACACGGCGAATGCCGCTGGGGTCGACCTGTACCTGCCCGGCGAGGAGTCCGAGCAGACCTACCACAGt                              >  6:431574/1‑135 (MQ=255)
                                                                                                      ggTCGAAGGCAATGAAATGATCCGCCGGGTGGGCTGGTGCACCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCTGACCGGCGAGGCGTCCGAGCAGACCTACCACAGTgc                            <  2:18032/135‑1 (MQ=255)
                                                                                                            aGGCAATTAAATGATCCGCCGTGTGGGCTGGTGCACCGTCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGTGTCGACCTGTACCTGACCGGCGAGGCGTCCGAGCAGACCTACCACAGTgctcg                         >  6:155731/1‑132 (MQ=255)
                                                                                                                             gccgGGTGGGCTGGTGCCCCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCTGACCGGCGAGGCGTCCGAGCAGACCTACCACAGTGCGCGCGAGAATGGCGTCAGCTTCa     <  6:349447/135‑1 (MQ=255)
                                                                                                                                ggggggGCTGGGGCACCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCTGACCGGCGAGGCGTCCGAGCAGACCTACCACAGTGCGCGCGAGAATGGCGTCAGCTTCATtg  <  4:28999/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                   
AAACCCCAAAGTGGTCGGGCTGATTGGTTCGCTCGCCGAATCCATGACCGCGCGCGATTTTGCCCGCCGGGTGCAACAGGTGCTGGGGCGTGAGCCGTTGCTGGTCGAAGGCAATGAAATGATCCGCCGGGTGGGCTGGTGCACCGGCGGCGGGCAGGGCTATATCGACACGGCGATTGCCGCTGGGGTCGACCTGTACCTGACCGGCGAGGCGTCCGAGCAGACCTACCACAGTGCGCGCGAGAATGGCGTCAGCTTCATTG  >  ACB122/1481883‑1482145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑