Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,780,7060AT46.4% 2.5 / 14.6 28intergenic (+96/‑29)ahpC_2/ahpFAlkyl hydroperoxide reductase C/Alkyl hydroperoxide reductase subunit F
Reads supporting (aligned to +/- strand):  ref base A (9/6);  new base T (12/1);  total (21/7)
Fisher's exact test for biased strand distribution p-value = 8.36e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCC‑GTCAAACGCCCGGGCGACCTCGCCTGGGCG‑TTTTTGTATCCGA‑GTTTTG‑AAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGGCGCGAAGTCCC  >  ACB122/2780590‑2780834
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gACCTCGTAGGAAAGATCTAAGGTCAGGCCAATAACGCGGGCGGTAAGCAGCCGCAAAAGCTTAAGTACCTACCGCCCG‑GTCAAACGCCCGGGCGACCTCGCCTGGGCG‑TTTTTGTATCCGA‑GTTTTG‑AAAAAgg                                                                                                                <  1:117843/135‑1 (MQ=255)
        aGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCC‑GTCAAAAGCCCCGGCGCCCTCGCCTGGGCG‑GTTTTTGATGCGA‑GGTTTG‑GAAAAGGGATGGccc                                                                                                        >  3:294822/1‑135 (MQ=255)
                       tCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCC‑GTCAAACGCCCGGGCGACCGCGCCGGGGCG‑GTTTTTGATACGC‑GCTTTG‑AAAAAGGTATGCGCCGTATGGTTGACGccc                                                                                         >  3:403348/1‑134 (MQ=255)
                        cctGCCCATACCGCGGGCGGTACGCAGCCGCCAAAGCTGAAGAACCTACCGCCCC‑GGCAAACGCCCGGGCGACCTCGCGTGGGCG‑TTTTTGTATCCGA‑GTTTTG‑AAAAAGGAATCGCCCGTATGTTGGACGCCAc                                                                                        <  5:116889/133‑1 (MQ=255)
                           gCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCC‑GTCAAAAGCCCGGGCGCCCACGCCAGGGCG‑GGTTTGTTTTCCA‑GGTTTG‑TAAAAAGAATAGCCCCGATGTTTGGCACCACGcg                                                                                     >  2:352525/1‑134 (MQ=255)
                            cccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGGTCAAACCCCCGGGCGCCCGCCCCTGGGCG‑GTTTTGTATCCGA‑GGTTTT‑AAAAATGGATGGCCCGTTTGTTGGACCCCAccc                                                                                      >  2:355448/1‑132 (MQ=255)
                            cccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCC‑GTCAAACGCCCGGGCGCCCTCGCCGGGGCG‑GTTTTGTTTACGA‑CGTTTT‑AAAAAAGAAAAGCCCGGATTGTGGACGccccccgt                                                                                    >  7:152478/1‑129 (MQ=255)
                            cccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCC‑GTCAAACGCCCGGGCGCCCACGCCGGGGCG‑TTTTTTTTTACGA‑GGTTTT‑AAAAATGTATAGCCCCGTTGTTGGGCGCCCCGcg                                                                                     >  1:144626/1‑133 (MQ=255)
                            cccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCC‑GTCAAACGCCCGGGCGACCTCGCCTGGGCG‑TTTTTGTTTCCGA‑GTTTTG‑AAAAAGGTAACGCCCCTAATTTGGGCCCCAAGcg                                                                                     >  4:40842/1‑133 (MQ=255)
                            cccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCC‑GTCAAACGCCCCGGCGCCCGCCCCAGGGCG‑TTTTTTTATAGGA‑GGTTTGTAAAAAGGAA‑AGCCCCGATGTTGGACGCCCCGcc                                                                                     >  5:152201/1‑133 (MQ=255)
                            cccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCC‑GTCAAACGCCCCGGCGCCCACGCCGGGGCG‑TTTTTTTTTCCGC‑GTTTTG‑AAAAAGGCAACCCCCCGATGTTGGGCGCCCCGcg                                                                                     >  5:171251/1‑133 (MQ=255)
                            cccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCC‑GTCAAACCCCCCGGCGCCCACGCCGGGGCG‑TGTTTTTTTACGAGGTTTTT‑AAAAAGG‑ATCGCCCCGTTGTTTGTCGCCCCGcg                                                                                     >  5:107399/1‑133 (MQ=255)
                            cccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCAC‑GTCAAAAGCCCGGGCGACCTCGCCTGGGCG‑TTTTTTTTTGCGA‑GTTTTT‑AAAAAAGGAAAGGCCGTATGTTGTCAGCCACGcgt                                                                                    >  7:300815/1‑133 (MQ=255)
                             ccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCC‑GTCAAACGCCCGGGCGCCCTCGCCTGGGCG‑GTTTTGTATGCGA‑GTTTTG‑AAAAAGGAAACGCCCGGATGTTGGACGCCACGCtt                                                                                    >  4:301090/1‑134 (MQ=255)
                             ccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCC‑GTCAAACGCCCCGGCGACCACGCCTGGGCC‑TTTTTGTATACCA‑GTTTTG‑AAAAAGGTATCGCCCGGTTGGTTGACGcccaccgt                                                                                    >  2:283433/1‑128 (MQ=255)
                             ccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCC‑GTCAAAACCCCGGGCGACCTAGGCAGGTCGTTTTTTTTTTCCTG‑GTTTTG‑‑AAAATGAATAGCCCGGATTTTTGACGGCaacgct                                                                                    >  3:63016/1‑129 (MQ=255)
                                    gcgggcggTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCC‑GTCAAACGCCCGGGCGACCTCGCCAGGGCG‑TTTTTTTTTCCTA‑GTTTTT‑AAAAAGGAATCGCCCGTATGTTGGACCCCCCGCGTTAAtagg                                                                             >  6:295509/1‑131 (MQ=255)
                                          ggTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCC‑GTCAAACGCCCCGGCGCCCTCGCCTGGGCG‑GTTTTGTTTCCGA‑GTTTTG‑GAAAAGGGAAAGCCCCGTTGTTGGGCGCCCCGCGTTAATCGCCACAGa                                                                       >  2:57712/1‑134 (MQ=255)
                                                     gccAAAGCTGAAGTACCTACCGCCCC‑GTCAAACGCCCGGGCGACCTCGCCTGGGCG‑TTTTTTTTTCCGC‑GTTTTG‑AAAAACGAATGGCCCGTATGTTGGGCGCCCCGCGTAAATGGCAACTGAAAACCAACtt                                                             >  1:316600/1‑133 (MQ=255)
                                                                      taccGCCCC‑GTCAAACGCCCGGGCGACCTCGCCTGGGCG‑TTTTTGTATCCGA‑GTTTTG‑AAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCg                                          <  4:218815/135‑1 (MQ=255)
                                                                         cGCCCC‑GTCAAACGCCCGGGCGACCTCGCCTGGGCG‑TTTTTGTATCCGA‑GTTTTG‑AAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATc                                       <  2:144626/135‑1 (MQ=255)
                                                                           cgcc‑GTAAACCGCCCCGCCGACCTCGCGTGGGGG‑TTTTTTTTTCGGT‑GTTTTT‑AAAACGCACTCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCga                                     <  2:218745/133‑1 (MQ=255)
                                                                                  cAAACGCCCGGGCGCCCTCGCCTGGGCG‑TTTTTGTTTCCGA‑GTTTTG‑AAAAAGGGATAGCCCGGATGTTGGGCGCCCCCCGTAAATCGCAACGGAAAACCTACTTGGAGCGGGTGCCCCAGCCGATCGAGATCGt                               >  2:59826/1‑135 (MQ=255)
                                                                                    aaCGCCCGGGCGACCTCGCGGGGGCT‑TTTTTGTAACCGA‑GTTTTG‑AAAAAGCAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTg                             <  6:354326/135‑1 (MQ=255)
                                                                                              cGACCTCGCCTGGGCG‑TTTTTGTATGCGA‑GGTTTG‑AAAAAGGAAACGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAATACCTACTTGGAGCGGGTCAACCAGCCGATCGAGATCGTTGGTTCCCTcga                   >  3:424561/1‑135 (MQ=255)
                                                                                                    cGCCTGGGCG‑TTTTTGTATCCGA‑GTTTTG‑AAAAGGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGCCGgc            >  7:70629/1‑136 (MQ=255)
                                                                                                    cGCCTGGGCG‑TTTTTGTATCCGA‑GTTTTG‑AAAAAGGAAACGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGCAAACCAACTTGGATCCGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCgacgaccg             >  5:174063/1‑133 (MQ=255)
                                                                                                               tttttGTATCCGA‑GTTTTG‑AAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGGCGCGAAGTccc  <  8:152478/136‑1 (MQ=255)
                                                                                                                      |                                                                                                                                  
GACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCC‑GTCAAACGCCCGGGCGACCTCGCCTGGGCG‑TTTTTGTATCCGA‑GTTTTG‑AAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGGCGCGAAGTCCC  >  ACB122/2780590‑2780834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑