| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 2,856,865 | 0 | T | G | 52.9% | 5.2 / 14.1 | 17 | W290G (TGG→GGG) | hdfR_4 | HTH‑type transcriptional regulator HdfR |
| Reads supporting (aligned to +/- strand): ref base T (6/2); new base G (0/9); total (6/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.07e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCACGACTATGCCAGCGACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGC > ACB122/2856741‑2856984 | tCACGACTATGCCAGCGACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCcg > 7:107130/1‑136 (MQ=255) ccAGCGACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCggggggggg > 5:250903/1‑129 (MQ=255) ccAGCGACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCggggggggg > 3:310262/1‑129 (MQ=255) ccgCGACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTGGCCGCGGGCCGGCTc < 5:195760/133‑1 (MQ=255) aCCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGGCGGCGCGcccc > 2:132809/1‑133 (MQ=255) cTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACCCTCACCTGACGCGCTAGGTCGTGCACCCGGCGCGCTGGGTGTTGCCGCGGGCCGGGCCGCCGcccc > 8:437648/1‑136 (MQ=255) gggCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGGGGGGGCGGCGGccccggc > 3:330392/1‑131 (MQ=255) aTGGTGGGCCTGGCCCGGCGGGGGCACCCGCTGTTCCACACTCACCTCACGCTCACGGGCGTGCACCCGGCGCGCGGGGTGTGGCCGCGGGCCGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTcgccgc < 4:310262/134‑1 (MQ=255) cggggggggCACCGGGTGTTCCACTCACACCTGCCGCTCACGGGCGTGCCCCGGGCGGGGGGGGTGTGGCCGCGGGCGGGCGCGCGGGCCGGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAgcg < 2:159764/131‑1 (MQ=255) cGGGGGCCCCCGCTGCTCCACACTCCCCGCCCGCTCCCGGGCGTGCACCGGGCGCGGGGGGGGGGCCGGCGGGCCGGCGCGCCGCCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGt < 7:399471/136‑1 (MQ=255) tGCACACTCACCTGCCGCTCACGGGCGTGCACCCGGCGCGCTGGGTGTTGCCGCGGGCCGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGaaa < 3:183714/135‑1 (MQ=255) cgCACACTCACCGCACGCACACGGGGGGGCCGCCGGCGCGCGGGGGGTGGCCGGGGGCCGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGaaa < 3:35544/134‑1 (MQ=255) ccacacaCCCCTGCCGCTCACGGGCGTGCACCCGGCGCGGGGGGTGTGGCCGCGGGCGGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGaaa < 3:112473/133‑1 (MQ=255) cacgggcgggcCCCCGGGGCGGGGGGGGGTGCCGCGGGCGGGCGCGCCGGCCCCTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTAt < 2:369891/132‑1 (MQ=255) cgggCGTGCCCCCGGGGGGGGGGGTGTGCCCGCGGGCGGGCGGGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATcc < 5:144787/134‑1 (MQ=255) ggCGTGCCCCCGGGGCGGGGGGGGGGGCGGCGGGCGGGCCCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGt < 3:39856/135‑1 (MQ=255) gTGCACCCGGCGCGCGGGGGGTGCCCGGGGGCCGGCTCGCCGGCCCGGCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGc < 4:94087/135‑1 (MQ=255) | TCACGACTATGCCAGCGACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGC > ACB122/2856741‑2856984 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |