Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,229,2020AC52.0% 1.1 / 11.9 25T414P (ACC→CCC) garD_1Galactarate dehydratase (L‑threo‑forming)
Reads supporting (aligned to +/- strand):  ref base A (12/0);  new base C (13/0);  total (25/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.50e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AAATCCCTGGGCGCAGTGGCCAAGGCAGGCTCCAGCAACCTGGTCGATGTGTATCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGCAACCAACAACCGGGTGTTCGAGCACCAGCAAGAGGACATG  >  ACB122/3229076‑3229336
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aaaTCCCTGGGCGCAGTGGCCAAGGCAGGCTCCAGCAACCTGGTCGATGTGTATCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCCCCGGTCAg                                                                                                                                >  1:406754/1‑135 (MQ=255)
aaaTCCCTGGGCGCAGTGGCCAAGGCAGGCTCCAGCAACCTGGTCGATGTGTATCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAgg                                                                                                                               >  7:289326/1‑136 (MQ=255)
            gcAGTGGCCAAGGCAGGCTCCAGCAACCTGGTCGATGTGTATCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCCCCGGTCAGGTGGCCGGCggg                                                                                                                    >  1:313744/1‑135 (MQ=255)
                  gCCAAGGCAGGCTCCAGCAACCTGGTCGATGTGTATCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGCCCCGGTCTCGGCCCCCGGTCAGGTGGCCGGCGGGGCCAAc                                                                                                              >  6:335463/1‑135 (MQ=255)
                       ggcaggcTCCAGCAACCTGGTCGATGTGTATCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCCCCGGTCAGGTGGCCGGCGGGGCCCCCCTGAt                                                                                                         >  3:72294/1‑135 (MQ=255)
                               ccAGCAACCTGGTCGATGTGTATCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCCCCGGTCAGGTGGCCGGCGGGGCCAACCTGCTCGCCTTcc                                                                                                 >  3:41167/1‑134 (MQ=255)
                               ccAGCAACCTGGTCGATGTGTATCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCCCCCGTCAGGTGGCCCGCGGGGCCCCCCTGATCGCCGTca                                                                                                 >  3:414483/1‑135 (MQ=255)
                               ccAGCAACCTGGTCGATGTGTATCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGCTCGCCTTcac                                                                                                >  7:107729/1‑136 (MQ=255)
                                                      cAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCCCCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCCCCGGCCGCGGCTCGGCCGACg                                                                         >  7:247680/1‑136 (MQ=255)
                                                       aGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCCCCGGTCAGGTGGCCGGCGGGGCCCACCTGATCGCCGTCCCCCCCCGCCCCGGCGCCGCCGAccg                                                                        >  8:301241/1‑134 (MQ=255)
                                                       aGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCCCCCGTCAGGTGGCCGGCGGGGCCACCCGGATCGCCTTCACCACCCGCCCCGGCTCGGCCGAcc                                                                         >  2:287746/1‑134 (MQ=255)
                                                       aGTACGCGCAAGCCGTGCGTGCCCAGGGCCAGGTGTTCATGGACACCACCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCGTCACCACCGGCCGCGGCGCGGccgtcg                                                                         >  6:3150/1‑131 (MQ=255)
                                                            gcgcAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGGCCCCGGTCCGGTGGCCGGCGGGGGCCACCTGCTCGCCTTCACCCCCGGCCGCGGCGCGGCCCGCGGcggg                                                                    >  2:102242/1‑132 (MQ=255)
                                                                aaGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCACCCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGcccc                                                               >  8:237190/1‑136 (MQ=255)
                                                                aaGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCCGTCAGGTGGCCCGCGGGGCCCCCCGGTTCGCCCTCACCCCCCGCCGCGGGCCGGCCCACGGCTGGGcac                                                                >  6:419387/1‑133 (MQ=255)
                                                                         gtgcCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCGTCCCCACCGGCCGCGGCTCGGCCTTCGGCGGTGCCCCGGCGCCag                                                       >  4:146314/1‑134 (MQ=255)
                                                                                   ccTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCCCCGGTCAGGTGGCCGGCGGGGCCACCCTGATCGCCTTCACCCCCGGCCGCGGCGCGGGCGACGGCGGGGCCCCCGCGCCCTCGAGCAAGcgg                                            >  7:87235/1‑134 (MQ=255)
                                                                                   ccTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCCCCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCGCGGCCGACGGCGGTGCCCCGGCGCCCTCGCTCCAGCt                                             >  4:147333/1‑135 (MQ=255)
                                                                                              tGGACACCCCCGGCTACGACCCGGTCTCGGCCACCCGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCCGCCCCGGCACGGCCAAACGCGGTGCCCCCGCCCCATCGCACAACCAGCcaacccacca                                 >  8:65499/1‑134 (MQ=255)
                                                                                               ggACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCCACCTGATCGTCTTCACCCCCGGCCGCGGCTCGGCCGACGGCGGTGCCCCCGCGCCCTCGCTCAAGCGGGCCACCaacaac                                >  8:294452/1‑136 (MQ=255)
                                                                                                       ccGGCTACGACCCGGTCTCGGCCCCCGGTCAGGTGGCCGGCGGGGCCCACCTGATCGCCGTCACCCCCGGCCGCGGCGCGGGCGGCCGCTGTGGCCCGGCGCCCGCGCTCCAGCGGGGACCCCACCACCCGgtgt                         >  4:147271/1‑135 (MQ=255)
                                                                                                       ccGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCGGTGCCCCGGCGCCCTCGCTCAAGCTGGCAACCAACAACCGGGGGtt                        >  7:223589/1‑136 (MQ=255)
                                                                                                            tACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCGACGGCTGGGCCCCGGCGCCCTCGATCCAGCTGGCAACCCACCACCGGGGGGTCgcg                    >  1:278689/1‑133 (MQ=255)
                                                                                                                        tcGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTGCGCCTACGGCTGGGGCCCGGCGCCCTCGATCAAGCTGGGCACCCCCACCCCGGTGTTCGCGCTCCAGCAACAgg       >  7:332472/1‑136 (MQ=255)
                                                                                                                        tcGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCGTCACCCCCGGCCGCGGCTCGGCCGACGGCGGTGGCCCGGCGCCCTCGCTCCAGCGGGCCACCAACAACCGGGTGGTGGCGCCCCCGCAAgggg       >  7:248940/1‑136 (MQ=255)
                                                                                                                              aCCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCCCGGCTCGGCCGACGGCTGTTCCCCGGCCCCATCGATCCACCGGGCAACCCAACACCCGGTGTTAGCGCACCACCAAGACGCCATg  >  2:80472/1‑135 (MQ=255)
                                                                                                                              |                                                                                                                                      
AAATCCCTGGGCGCAGTGGCCAAGGCAGGCTCCAGCAACCTGGTCGATGTGTATCAGTACGCGCAAGCCGTGCGTGCCCAGGGCCTGGTGTTCATGGACACCCCCGGCTACGACCCGGTCTCGGCCACCGGTCAGGTGGCCGGCGGGGCCAACCTGATCGCCTTCACCACCGGCCGCGGCTCGGCCTACGGCTGTGCCCCGGCGCCATCGATCAAGCTGGCAACCAACAACCGGGTGTTCGAGCACCAGCAAGAGGACATG  >  ACB122/3229076‑3229336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: