| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,440,348 | 0 | T | G | 54.2% | 1.4 / 12.6 | 28 | A19A (GCT→GCG) | CJ019_03266 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (9/2); new base G (8/5); total (21/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAA > ACB122/3440217‑3440473 | cAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTggcg > 7:435661/1‑136 (MQ=255) gtAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCGCGCgccgccgc > 5:400623/1‑133 (MQ=255) gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCgggggg > 7:177086/1‑134 (MQ=255) gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCCCCCCGGCAcccagcgg > 6:154799/1‑130 (MQ=255) gCAACGGTCTGGAGGATCGCAAGGCACTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCAGGCGGCGCCCGCGCCGCCCggcg > 1:279668/1‑133 (MQ=255) ccAACCGACTGGAGGATCGCAAGCCCCTCTACAAGCGAGCGCTCGAGCAGCTGCAGAGAGCGCATCCGCCACCAGCCTGGTCGCCGTGGCGCTGCTGCTGGACACCACCGCAGCCCGCGCCCCGGCCCCCTgaggg > 8:248292/2‑132 (MQ=255) ggTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACACGCCTTGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCAGGGGGCGCCCGGGCGACCCGGGGGTgggc > 8:324718/1‑134 (MQ=255) tGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGCCGGCGCCCCCGCACCCGGGGGGGTGCAGGc > 6:34529/1‑135 (MQ=255) cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGGGGCGCCCGGGCCGCCTGGGGGTGGGAGGCCCCCGccga > 3:117738/1‑132 (MQ=255) aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCCGCCGGGGGGTGGCGGGCCCACGCCGATaa > 8:136122/1‑136 (MQ=255) aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCAGGCCGCGCCCCCGCACCCTGGGTGTGGGAGGCCCCCGccga > 3:192430/1‑131 (MQ=255) ctacccgcgcgcgcTCGCGGTGCGGCAGTGAGCGCGGGGGCCCCCCGCCTGGGCGCCGTGGCGCTGCTGCGGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGc < 7:67463/130‑1 (MQ=255) agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGGCCGGGCCGCCGGGGGGTGGGGGGCCCACGCCTATAAGGAGCCGCTCGcgggt > 1:255267/1‑131 (MQ=255) agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGCCGGCCCACCCCGATAAGGCGCCgcgcgcggct > 8:338517/1‑131 (MQ=255) gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGGAGGCCCCCGCCGATAAAGCGCAGCACGCGGCTCac > 1:312275/1‑134 (MQ=255) gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCGGGGTGTGGCAGGCCAACGCCTAGAAGGCGCAGCTCGCTGATCAgg > 7:51453/1‑136 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCGGGCGGCGCCCCCGCCGCCCGGGGGTGGCGGGGCCACCCCAATAACGCGCCGCTCGCGGGTCAGGc > 1:68860/1‑135 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGGGGGGCGGGGCCACGCCGATAAGGCGCAGCGCGCGGCTCAGGc > 1:242162/1‑135 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCCGGCCGCCTGGGGGTGGGCCGCCCCCCCCTATAATGCGCCGCTCGCTGGTCAgggc > 8:178051/1‑134 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCGCGCGCGCGGCGCCCGGGCCGCCGGGGTGGGGCCGGCCCCCGCCGAGAATGCGCAGCGGGCGGATCAggg > 5:436981/1‑134 (MQ=255) gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGGCGCCGGGGTGTGGCAGGCCCACCCCGATAATGCGCCGCTCGCGGGTCAGGCCGCTGACGTAGCCAAGCAg > 2:354980/1‑135 (MQ=255) gCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGGAGGCCCACGCCGATAAGGCGCCGCGCGCGGATCAGGCCGAGGGCTTCGGCGCGCCGTTGGGGGAGaa > 5:173801/1‑133 (MQ=255) gggcggTGGGGCTGCGGCGGGCCGCGCGGGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 7:338517/131‑1 (MQ=255) gggggTGGCGCTGCGGCGGGCGGCCGGGGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 5:154799/131‑1 (MQ=255) cGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGTCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 1:284812/135‑1 (MQ=255) ccggCCGCCCGCGCGGCCGCCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCgg < 1:109035/133‑1 (MQ=255) ggCCGCGCGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGga < 1:394100/135‑1 (MQ=255) cgccAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa < 4:417784/135‑1 (MQ=255) | CAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAA > ACB122/3440217‑3440473 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |