Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,440,3630AC53.8% 6.5 / 21.6 27A24A (GCA→GCC) CJ019_03266hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (4/8);  new base C (14/0);  total (19/8)
Fisher's exact test for biased strand distribution p-value = 3.17e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCA  >  ACB122/3440234‑3440497
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gtAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCGCGCgccgccgc                                                                                                                                   >  5:400623/1‑133 (MQ=255)
   gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCgggggg                                                                                                                               >  7:177086/1‑134 (MQ=255)
   gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCCCCCCGGCAcccagcgg                                                                                                                                >  6:154799/1‑130 (MQ=255)
   gCAACGGTCTGGAGGATCGCAAGGCACTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCAGGCGGCGCCCGCGCCGCCCggcg                                                                                                                                >  1:279668/1‑133 (MQ=255)
   ccAACCGACTGGAGGATCGCAAGCCCCTCTACAAGCGAGCGCTCGAGCAGCTGCAGAGAGCGCATCCGCCACCAGCCTGGTCGCCGTGGCGCTGCTGCTGGACACCACCGCAGCCCGCGCCCCGGCCCCCTgaggg                                                                                                                               >  8:248292/2‑132 (MQ=255)
        ggTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACACGCCTTGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCAGGGGGCGCCCGGGCGACCCGGGGGTgggc                                                                                                                          >  8:324718/1‑134 (MQ=255)
            tGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGCCGGCGCCCCCGCACCCGGGGGGGTGCAGGc                                                                                                                       >  6:34529/1‑135 (MQ=255)
                      cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGGGGCGCCCGGGCCGCCTGGGGGTGGGAGGCCCCCGccga                                                                                                              >  3:117738/1‑132 (MQ=255)
                       aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCCGCCGGGGGGTGGCGGGCCCACGCCGATaa                                                                                                           >  8:136122/1‑136 (MQ=255)
                       aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCAGGCCGCGCCCCCGCACCCTGGGTGTGGGAGGCCCCCGccga                                                                                                              >  3:192430/1‑131 (MQ=255)
                               ctacccgcgcgcgcTCGCGGTGCGGCAGTGAGCGCGGGGGCCCCCCGCCTGGGCGCCGTGGCGCTGCTGCGGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGc                                                                                                   <  7:67463/130‑1 (MQ=255)
                                        agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGGCCGGGCCGCCGGGGGGTGGGGGGCCCACGCCTATAAGGAGCCGCTCGcgggt                                                                                           >  1:255267/1‑131 (MQ=255)
                                        agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGCCGGCCCACCCCGATAAGGCGCCgcgcgcggct                                                                                           >  8:338517/1‑131 (MQ=255)
                                           gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGGAGGCCCCCGCCGATAAAGCGCAGCACGCGGCTCac                                                                                        >  1:312275/1‑134 (MQ=255)
                                           gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCGGGGTGTGGCAGGCCAACGCCTAGAAGGCGCAGCTCGCTGATCAgg                                                                                       >  7:51453/1‑136 (MQ=255)
                                             tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCGGGCGGCGCCCCCGCCGCCCGGGGGTGGCGGGGCCACCCCAATAACGCGCCGCTCGCGGGTCAGGc                                                                                      >  1:68860/1‑135 (MQ=255)
                                             tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGGGGGGCGGGGCCACGCCGATAAGGCGCAGCGCGCGGCTCAGGc                                                                                      >  1:242162/1‑135 (MQ=255)
                                             tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCCGGCCGCCTGGGGGTGGGCCGCCCCCCCCTATAATGCGCCGCTCGCTGGTCAgggc                                                                                     >  8:178051/1‑134 (MQ=255)
                                             tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCGCGCGCGCGGCGCCCGGGCCGCCGGGGTGGGGCCGGCCCCCGCCGAGAATGCGCAGCGGGCGGATCAggg                                                                                      >  5:436981/1‑134 (MQ=255)
                                                                gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGGCGCCGGGGTGTGGCAGGCCCACCCCGATAATGCGCCGCTCGCGGGTCAGGCCGCTGACGTAGCCAAGCAg                                                                   >  2:354980/1‑135 (MQ=255)
                                                                             gCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGGAGGCCCACGCCGATAAGGCGCCGCGCGCGGATCAGGCCGAGGGCTTCGGCGCGCCGTTGGGGGAGaa                                                        >  5:173801/1‑133 (MQ=255)
                                                                                   gggcggTGGGGCTGCGGCGGGCCGCGCGGGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc                                               <  7:338517/131‑1 (MQ=255)
                                                                                    gggggTGGCGCTGCGGCGGGCGGCCGGGGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc                                               <  5:154799/131‑1 (MQ=255)
                                                                                    cGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGTCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc                                               <  1:284812/135‑1 (MQ=255)
                                                                                                    ccggCCGCCCGCGCGGCCGCCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCgg                               <  1:109035/133‑1 (MQ=255)
                                                                                                      ggCCGCGCGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGga                             <  1:394100/135‑1 (MQ=255)
                                                                                                         cgccAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa                          <  4:417784/135‑1 (MQ=255)
                                                                                                                 cggccggcgCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTgcagc                 <  7:136122/132‑1 (MQ=255)
                                                                                                                                 aGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGACGGCTGCAGCAGCAGCGCTCGATCa  >  5:286596/1‑135 (MQ=255)
                                                                                                                                 |                                                                                                                                      
GTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCA  >  ACB122/3440234‑3440497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑