| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,491,337 | 0 | G | C | 56.0% | 20.4 / 34.4 | 25 | intergenic (‑699/+22) | CJ019_03306/CJ019_03307 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (3/8); new base C (14/0); total (17/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.53e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.46e-06 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCCGTTCATCGTT > ACB122/3491202‑3491463 | cTACATCGACCGGTGGTGTCGCCAGGTGGTAGGCGCTGGTTAACCCGCGAATAGGCCGGACCTGCCGGCCAATATCCCTGGGCATTCACCGTCACACCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCg < 8:446404/136‑1 (MQ=21) tACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCCGGCCGCCGGCCCGCGAACACCg > 6:81992/1‑135 (MQ=255) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTATCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACCGCCGCCACCGCATTCCCGGGCAGCCGGCCCGCCCACACCCGCGCAGcccg > 6:91523/1‑133 (MQ=21) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCCGCCGGCCCGCGCACACCCgcgccccccg > 2:382716/1‑129 (MQ=21) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCCGGCCGCCCGCCCCCCCACACCCGCgccgcccg > 5:423963/1‑133 (MQ=18) ccACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCCGCCGGCCCGCGCACCCCCGCGCCCCCGGGTTCATCCaa > 7:201780/1‑135 (MQ=18) cgggTTTACCCGCTAATAGGCCGGACCTGCCGGCCACAATCCCTGGTCATTCACCGACACCGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGa < 7:323047/134‑1 (MQ=37) ccccGCTAATTGGCCGCACCTGCCGCCAACTACCCGTGGCCATTCCCCGCCACCGCCGCCCCCCCATGCGGGGGCCGCCGGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTAc < 7:74246/135‑1 (MQ=255) aCCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCCCGGGCACCCTGCCCCCGCACAACCGCGCAGCCGGGTTGAGACAACTCAACGCCCCTCGcccaggcc > 7:121321/1‑131 (MQ=18) gcgAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCCGCCGGCCCGCGAACAACCGCGCCGCCGGGTTCATCCAACTCAACGCCCAACCCCCTGTACGc > 4:186083/1‑134 (MQ=21) tAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCCCGGGGACCCGCGCCGCGAACACCCGCCCACCCGCTGTCATCCAACAGCACGACCAAGGACCGGTGCGCAAAccg > 4:230603/1‑133 (MQ=12) gCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCCGCCTGCCCGCCAACACCCGCCCCGCCGGGGTCATCCAACTCAACGACCATCGACCAGTAAGCAAAAGTCTCTGCCCTCGaattc > 6:407556/1‑131 (MQ=25) cTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCCGGCCGCCTGCCCGCGAACACCCGCGCAGCCGGTTTGATCCAACACAACCACCCTCGCCCGGGACGCCAACGTCTCTGCCAACGATTTCCATGCATggg > 2:82994/1‑134 (MQ=255) aTTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCCGCGCCGCCGGGTTGATCCAACACAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGAATGCCTTGCATGGGCAAtag > 4:46207/1‑133 (MQ=255) tCACCGTCACAGCCGCCACCGCATTCGCCGGCACCCTGACCGCGCAAAACCCCGCAGACCGTGTCAGACAACAAAAAGACCACCCACCGGTTCCACAACCTCTCTGCGAAACCTTGCCTTGGAAGGCCCATTGta > 6:151356/1‑134 (MQ=255) aCCGTCACAGCCGCCACCGCATTCGCGGGCCGCCGGCCCGCGAACACCCGCGCAGCCCGTTTGATACAACTCAACGCCCATCGACCAGTTCGCAAACCTCTCTGCCATCGATTGGCGTGGATGGCCAATTGTCgg > 2:47731/1‑134 (MQ=255) aCCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCCGCGCCGCCGGGTTCATCCAACACAACGCCCCTCGCCCTGTACGCCAACGTCTATGCCATCGATTGGCTTGCATGGCCAATTGTGGt > 4:126171/1‑135 (MQ=255) ccGTCACAGCCGCCACCGCATTCGCGGGCAGCATGCCCCCGAAAACCGGCGCAGCCCGGTTCATACAACACAACGCCCATAGACCAGTACGCCAACGTAGCTGCCATCGCTTGCCTTGCAGGGACAATTTTCGTTg > 8:96564/1‑136 (MQ=255) gcccccACGGCATGCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAAt < 3:203123/135‑1 (MQ=255) cccgccCCCGCTTCGGCGGGCCGCCGGCCCGCCAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAAt < 3:20283/134‑1 (MQ=255) aCCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCg < 4:72273/135‑1 (MQ=255) aCCGCATTCGCGGGCAGCCGGCCCGCGAACACCCGCGCCGCCGGGTTGATCCAACTCAACGCCCCTCGCCCGGTCCGCCCAAGGCTCGGCCCTCGCTTTCCTTGCCTGGGCCATTTTGGTTGATGTTATGATCCg > 2:159119/1‑135 (MQ=255) ccccgccccGCAAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACAccc < 5:39350/129‑1 (MQ=255) ccggcgAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCCGTTCAt < 1:382716/132‑1 (MQ=255) ccGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGCTCCGGCTTGAAGCCCACCCGTTCATc > 2:189164/1‑135 (MQ=255) ccGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCCGTTCa < 3:126171/133‑1 (MQ=255) gcgAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCCGTTCATCGtt > 8:51112/1‑136 (MQ=255) | CTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCCGTTCATCGTT > ACB122/3491202‑3491463 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |