| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 5,165,184 | 0 | T | G | 56.2% | 3.0 / 14.2 | 20 | intergenic (+137/+46) | puuB_6/fadD_1 | Gamma‑glutamylputrescine oxidoreductase/Long‑chain‑fatty‑acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base T (0/7); new base G (9/0); total (13/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTACAGGGACTCACAATGTCTGAAGACGGCGCCGCATCTTATTGTGGGGCCTTGTGTCGCGAAAGGGGCGCAAAGCG‑CCCCCAGCCATTGATGCATCGCCGCCGAAATCCCAGGGCCGCGTCGCGGCCCTTTCGCGACACGAGGCCGCCCCCACACTGAGCGTGCAGGCTTGTGGTCAGGCGATCTTCTTCAAGCCTTGCTTCTTGAGCTCTTCATCACGCAGTTCGCGGCGCAGGATCTTGCCCACGTTGGT > ACB122/5165055‑5165307 | cTACAGGGACTCACAATGTCTGAAGACGGCGCCGCATCTTATTGTGGGGCCTTGTGTCGCGAAAGGGGCGCAAAGCG‑CCCCCAGCCATTGATGCATCGCCGCCGAAATCCCAGGGCCGCGTCGCGGCCCGTTcgc > 2:302921/1‑135 (MQ=255) cacaATGTCTGAAGACGGCGCCGCATCTTATTGTGGGGCCTTGTGTCGCGAAAGGGGCGCAAAGCG‑CCCCCAGCCATTGATGCATCGCCGCCGAAATCCCAGGGCCGCGTCGCGGCCCGTTGGCGGCACCACgcc > 5:164895/1‑135 (MQ=39) cacaATGTCTGAAGACGGCGCCGCATCTTATTGTGGGGCCTTCTGACGCGAAAGGGGCGCAAGGCG‑CCCCCAGCCATTGATGCATCGCCGCCGAAAACCCAGGTCCGCGTCCCGGCCCATTCGAGACACCCGgcc > 4:78139/1‑135 (MQ=255) aTGTCTGAAGACGGCGCCGCATCTTATTGTGGGGCCTTGTGTCGCGAAAGGGGCGCAAAGCG‑CCCCCAGCCATTGATGCATCGCCGCCGAAATCCCAGGGCCGCGTCGCGGCCCGTTCGCGGAACCAGGCCGccc > 4:185895/1‑135 (MQ=255) gAAGACGGCGCCGCATCTTATTGTGGGGCCTTGTGTCGCGAAAGGGGCGCAAAGCG‑CCCCCAGCCATTGATGCATCGCCGCCGCAATCCCCGGGCCGCGTCGCGGCCCGTTTGGGCCCCGCGGCCGccccccccc > 6:87951/1‑131 (MQ=37) gAAGACGGCGCCGCATCTTATTGTGGGGCCTTGTGTCGCGAAAGGGGCGCAAAGCG‑CCCCCAGCCATTGATGCATCGCCGCCGAAATCCCAGGGCCGCGGCGCGGCCCCGTGGCGCCCCGCGGcccccccccccc > 5:430351/1‑131 (MQ=37) gccgcATCTTATTGTGGGGCCTTGTGTCGCGAAAGGGGCGCCAAGCG‑CCCCCAGCCATTGATGCATCGCCGCCGAAATCCCAGGGCCGCGTCGCGGGCCCTTGGGGCCCCGCGGGCCCCCCCCCCCAGAGCGGGc > 6:125118/1‑135 (MQ=25) gccgcATCTTATTGTGGGGCCTTGTGTCGCGAAAGGGGCGCAAAGCG‑CCCCCAGCCATTGATGCATCGCCGCCGAAATCCCAGGGCCGCGTCGCGGCCCGTTACCGACCCCAGGCCGCCCCCCCACAGAGCCTggc > 7:56428/1‑134 (MQ=38) gccgcATCTTATTGTGGGGCCTTGTGTCGCGAAAGGGGCGCAAAGCG‑CCCCCAGCCATTGATGCATCGCCGCCGAAATCCCAGGGCCGCGTCCCGGCCCCTTCCCGCCCCGAGGCCCCCCCCCCACAGAGCGGGc > 4:242047/1‑135 (MQ=37) tgtgGGGCCTTGTGTCGCGAAAGGGGCGCAAAGCG‑CCCCCAGCCATTGATGCATCGCCGCCGAAATCCCAGGGCCGCGTCGCGGCCCGTTGGCGACCCGCGGCCGCCCCCCCCCCGAGCGTGGCGGCTTGTggggc > 8:414823/1‑133 (MQ=37) caccgggCCCAAACCGCCCCCCAGCCATTCAGGCAGCCCCGCCAAAACCCCCGGCCGGCGCGGCGGCCCTTTCGCGACACGAGGCCGCCCCCACACTGAGCGTGCAGGCTTGTGGTCAGGCGATCTTCTTCAAGc < 4:329274/131‑1 (MQ=255) aaGGGGCGCAAAGCG‑CCCCCAGCCATTGATGCATCGCCGCCGAAATCCCAGGGCCGCGTCGCGGCCCGTTCGCGCCCCGCGGCCGCCCCCCCCCGGAGCGTGCAGGCGTGTGGGGAGGCGGTGTTCTTCAAGCCt > 2:7233/1‑135 (MQ=255) aaaGCG‑CCCCCAGCCATTGATGCATCGCCGCCGAAATCCCAGGGCCGCGTCGCGGGCCGTTTGCGGCCCGCCGCCCCCCCCCCACGTCGCCTGCCGGGGTGTGGTGAGGGGATCTTCTTAAAGCCGTgggtga > 2:18884/1‑128 (MQ=255) aacgcccccccAGCCATTGAAGCCAGGCCGCCGAAACCCCCGGGCCGCGCCGCGGCCGTTTCGCGACACGAGGCCGCCCCCACACTGAGCGTGCAGGCTTGTGGTCAGGCGATCTTCTTCAAGCCTTGCTTCTTGa < 8:125076/130‑1 (MQ=255) aGCCATTGAGGCAGCGCCGCCTAAAGCCCGGGGCCGCGGCGGGCCCCTTACGCGACACGAGGCCGCCCCCACACTGAGCGTGCAGGCTTGTGGTCAGGCGATCTTCTTCAAGCCTTGCTTCTTGAGCTCTtcatc < 4:123374/135‑1 (MQ=255) cgcccgcgccgcGCAAACCCCGGGGCCGCGGGGGGGCCCTTTCGGGACACGAGGCCGCCCCCACACTGAGCGTGCAGGCTTGTGGTCAGGCGATCTTCTTCAAGCCTTGCTTCTTGAGCTCTTCATCACGCAGt < 2:302353/128‑1 (MQ=255) cgccgcCGAAATCCCAGGGCCGCGTCGCGGCCCGTTAGCGGCACGAGGCCGCCCCCACACTGACCGTGCACGCTTGTGGTCAGGCGATCGTCTTCAAGCCTTGCTTCTTGAGCTCTTCAACACGCCTTTcgccgc > 6:203646/1‑135 (MQ=255) gAAACCCCGGGGCGGCGCGGCGGCCCTTGCGCGACACGAGGCCGCCCCCACACTGAGCGTGCAGGCTTGTGGTCAGGCGATCTTCTTCAAGCCTTGCTTCTTGAGCTCTTCATCACGCAGTTCGCGGCGCAGGAt < 2:84114/135‑1 (MQ=255) acccccGGCCCGGGGCGCGGCCCTGACGCGACAGGAGCCCGCCCCCACACTGAGCGTGCAGGCTTGTGGTCAGGCGATCTTCTTCAAGCCTTGCTTCTTGAGCTCTTCATCACGCAGTTCGCGGCGCAGGATCtt < 4:99123/133‑1 (MQ=255) ggccggggCCTTTTCGCGACAGGTGGCCGCCCCCACACTGAGCGTGCAGGCTTGTGGTCAGGCGATCTTCTTCAAGCCTTGCTTCTTGAGCTCTTCATCACGCAGTTCGCGGCGCAGGATCTTGCCCTCGTtggt < 2:142607/129‑1 (MQ=255) | CTACAGGGACTCACAATGTCTGAAGACGGCGCCGCATCTTATTGTGGGGCCTTGTGTCGCGAAAGGGGCGCAAAGCG‑CCCCCAGCCATTGATGCATCGCCGCCGAAATCCCAGGGCCGCGTCGCGGCCCTTTCGCGACACGAGGCCGCCCCCACACTGAGCGTGCAGGCTTGTGGTCAGGCGATCTTCTTCAAGCCTTGCTTCTTGAGCTCTTCATCACGCAGTTCGCGGCGCAGGATCTTGCCCACGTTGGT > ACB122/5165055‑5165307 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |