Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB122	44,301	(T)9→8	intergenic (+132/‑359)	oprD_1 → / → czcR_2	Porin D/Transcriptional activator protein CzcR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	44,293	0	T	.	89.7%	138.5 / 14.8	40	intergenic (+124/‑367)	oprD_1/czcR_2	Porin D/Transcriptional activator protein CzcR
Reads supporting (aligned to +/- strand):  ref base T (1/3);  new base . (16/19);  total (18/22)
Fisher's exact test for biased strand distribution p-value = 6.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGATTCTGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCGGAA  >  ACB122/44157‑44414                                                                                                                                         |                                                                                                                          ttCGATTCTGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCt                                                                                                                           >  5:78755/1‑137 (MQ=255)        cTGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAACACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCACATTGGGCGCCtttttttt                                                                                                                    <  8:49272/137‑1 (MQ=255)            aaTTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCttttttttgcct                                                                                                                >  2:269875/1‑133 (MQ=255)             aTTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTa                                                                                                              >  8:238213/1‑137 (MQ=255)                 gCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAAt                                                                                                          >  8:29554/1‑137 (MQ=255)                      cTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAg                                                                                                     <  5:186672/137‑1 (MQ=255)                      cTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGAGATCCTTTGGTAAAATGAGGATATTTGTCGACCATTTGCCGCC‑GTTTTTTTGCCTAAAAATCaat                                                                                                     >  8:177873/1‑136 (MQ=255)                         ggCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAg                                                                                                  <  2:240264/137‑1 (MQ=255)                                            ggTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcgcatc                                                                               <  5:216003/137‑1 (MQ=255)                                            ggTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcgcatc                                                                               <  4:66186/137‑1 (MQ=255)                                               ccTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcgcatcgca                                                                            >  1:156301/1‑137 (MQ=255)                                                 tGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATATGGCGCCCATTGGGCGCCTATTTTTTT‑CCTTAAAATAAAATATGCGTTAGAATGTAagcatcccatt                                                                          >  1:301128/1‑136 (MQ=255)                                                    tAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                       <  7:29554/137‑1 (MQ=255)                                                    tAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                       <  6:254602/137‑1 (MQ=255)                                                    tAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCG‑CTTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                       <  6:142354/137‑1 (MQ=255)                                                          aaCGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGACGGTGAAg                                                                 >  6:276013/1‑137 (MQ=255)                                                           aCGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCCTCGCATGCCGGTGAAGt                                                                >  1:284853/1‑137 (MQ=255)                                                                cATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATg                                                           >  2:142983/1‑137 (MQ=255)                                                                cATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGAGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATg                                                           >  6:127240/1‑137 (MQ=255)                                                                  ttACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGa                                                          <  3:159137/137‑1 (MQ=255)                                                                           cACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAAt                                                <  8:156627/137‑1 (MQ=255)                                                                             ccTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGa                                              >  3:245613/1‑137 (MQ=255)                                                                              cTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAc                                             <  4:140531/137‑1 (MQ=255)                                                                              cTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAc                                             <  6:39309/137‑1 (MQ=255)                                                                              cTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAc                                             >  3:233302/1‑137 (MQ=255)                                                                               ttGAGTGGCTTAAGTGCTCCTTTGGTTAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAcc                                            <  2:110050/137‑1 (MQ=255)                                                                               ttGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAcc                                            <  6:92592/137‑1 (MQ=255)                                                                                tGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCa                                           <  1:38046/137‑1 (MQ=255)                                                                                tGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCa                                           >  8:117135/1‑137 (MQ=255)                                                                                tGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCa                                           <  1:269875/137‑1 (MQ=255)                                                                                    tGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGtct                                       <  4:245613/137‑1 (MQ=255)                                                                                    tGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGtct                                       <  4:233302/137‑1 (MQ=255)                                                                                       cTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTGAAGTAGGCGTTCGATTGTACGCATCGCATGACGGTGAAGTTAATGACTACACCAATGACCAGTCTCtt                                    >  1:219453/1‑137 (MQ=255)                                                                                         tAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATTACTACACCAATGACCAGTCTCTTCa                                  >  8:142738/1‑137 (MQ=255)                                                                                          aaGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCaa                                 >  5:127341/1‑137 (MQ=255)                                                                                          aaGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCaa                                 >  8:2423/1‑137 (MQ=255)                                                                                             ttgtacTTTGGTATAAGTTGGTTATTTGGAGACCATTGTGCTCC‑TTTTTTTTGCCTTATATTCATATACGCTTTCGATTGTACTCATCGCATGCCGGTGAAGTTCATGACTTCAGCAATGACCAGTCTCTTGAAtgt                              <  2:280839/132‑1 (MQ=255)                                                                                                  cTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCg                         <  7:236388/137‑1 (MQ=255)                                                                                                    ttGGTAAAAGGTGGATATTTGGCGCCCATTGGGCG‑CGTTTTTTTTGCCTAAAATTAAAGTAGGCGTTAGATTGTACGCATCGCCGTCCGGTGAAGTTCATTTCTACACAAAAGACCAGTCTCTTGAATGTTAGCggc                       >  4:276515/1‑135 (MQ=255)                                                                                                                  atatTTGGCGCCCATTGGGCGCCTTTTTTTTT‑CCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTg         <  6:78755/137‑1 (MQ=255)                                                                                                                  atatTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTg         <  4:166064/137‑1 (MQ=255)                                                                                                                       tGGCGGCCATTGGGCTCC‑TTTTTTTTGCCTAAAATTGAAGTAGGAGTTCGATTGTACTCATCGCATGCCGGTGAAGTTCATGACTACACCTATGACCAGTGTCTTGTATGTGAGCGACCTCGACGTCATCTGAGCgg    <  5:198384/137‑1 (MQ=255)                                                                                                                         gcgcCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCGGaa  >  2:45823/1‑137 (MQ=255)                                                                                                                                         |                                                                                                                          TTCGATTCTGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCGGAA  >  ACB122/44157‑44414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑