Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122434,3760G.25.0% 49.7 / 14.2 20coding (207/1173 nt)bioF8‑amino‑7‑oxononanoate synthase
Reads supporting (aligned to +/- strand):  ref base G (8/7);  new base . (5/0);  total (13/7)
Fisher's exact test for biased strand distribution p-value = 1.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCCAT  >  ACB122/434243‑434510
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aacccccGCACGGGCCGGAAGGGGGGGGCGCCGGCCAGCGGTGGCTGGCCTTCTGCACCAATGACAACCTGGGCCTGGCCAACCACCCCGCGGTGATCGCCGCCTGGCAGGCCGGCGCCGCGCGCgggggggggggg                                                                                                                                     <  2:54346/133‑1 (MQ=255)
   gcacGCAAGGGCCGGAAGTGGTGGTCGACGGCCACCCGTTTCTTGGCTTCTGCACCCATTACTAACTGGGCCAGGCCCACCCCCCCGTGGTGATAGCCGCCATGCCCGCCGGCGGCCCCCGCGGGGGGGTggggggg                                                                                                                                  >  8:135541/4‑137 (MQ=255)
       gCAAGGGCCGGAAGTGGTGGTCGACGGCCCGCCGTTGCGGGCCGTCTGCCGCAATGACAAACGGGGCCGGGCCAACCACCCCGAGGTGATAGCCGCCTGGGAGGCCGGCGCCGAGCGCGGGGGTGTggggggggggg                                                                                                                              >  2:76794/1‑137 (MQ=255)
          tgggCCGGAAGTGGGGGGCGCCGGCCCGCGGTTGCTGGCATTCAGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGCGGTGCGCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTggggggggggcctc                                                                                                                           <  2:146445/136‑5 (MQ=255)
            ggCCGGAAGTGGTGGTCGACGGCCCCCGGTTTGTGGGCTGGTGGAGGAATTACAACCGGGGGCAGGCCAAACCCCCCCCGGGGATAGCCCCCGGGGCGGCCGGCGCCCCGCGCGGGGGGGTggggggggggggcgcg                                                                                                                         >  6:1231/1‑132 (MQ=255)
            ggCCGGAAGTGGTGGTCGACGGCCAGCGGTTGGTGGGCGTCTGGAGCAATGACGACCTGGGCCTGGCCAACCACCCCGAGGTGATAGCCGCCTGGCCGGCCGGCGCCGCGCGCGGGGGTGTggggggggggggcgcg                                                                                                                         >  6:259351/1‑132 (MQ=255)
                           gggccGGCCAGGGGGTGCTGGCCTTCTGCACCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGC‑GGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGcc                                                                                                         <  8:57879/133‑1 (MQ=255)
                                  cccggggTTGGGGGCCGTCTGCACCAATCCCTACCTGGGCCTGGCCAACCCCCCCGAGGGGATCCCCGCCGGGCCGGCCGGCGCCGCGCGCGGGGGGGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCa                                                                                                   <  2:93305/132‑1 (MQ=255)
                                         ttggcggCCGTCTGCACCAATGACACCCTGGGCCCGCCCAACCCCCCCGCGGTGACCGCCGCCGGGCCGGCCGGCGCCGCGCGGGGGGGGGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCa                                                                                            <  3:181655/132‑1 (MQ=255)
                                                       cagcaATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCAACAGGTGGAaga                                                                             >  1:284765/1‑137 (MQ=255)
                                                               aCTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCGCGCCCCTGGAAGTGGCCCACACCCCCCCCCCCCAGCAGGTTGAAGAGGCAcgg                                                                      >  8:134629/1‑135 (MQ=255)
                                                                  aCCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCt                                                                   >  4:267761/1‑137 (MQ=255)
                                                                               cAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCCCCTGGTAGTTGGCCACCGCCCCCCCCCCCATCAGGTTGAAGAGGCACGGGCGGAACACACCGgcc                                                      >  4:110452/1‑137 (MQ=255)
                                                                                    accCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCGGCGCCCGGGGTAGTTGGCCCCCCCCCCCCGCCCCAGCCGGTGGAAGGGGCCGGGGCGGAACGCACCGGccgcccc                                                 >  1:189451/1‑136 (MQ=255)
                                                                                    accCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGccgcccgc                                                >  3:94550/1‑137 (MQ=255)
                                                                                     ccccGGGGGGATCGCCGCCTGGCCGGCCGGGGCCGCGCGGGGGGGGGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGccgcccgc                                                <  2:122846/137‑1 (MQ=255)
                                                                                     ccccGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAAGTCACCGGccgcccgc                                                >  3:140593/1‑137 (MQ=255)
                                                                                     ccccGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCCCCcgcg                                               >  5:253653/1‑137 (MQ=255)
                                                                                          aGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCCCAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCCCGCGcc                                           >  4:210467/1‑137 (MQ=255)
                                                                                                  gccgccTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCCCCTGGTAGTTGGCCACAGCACCCCGCACCAGCAGGTTGAAGCGGCCGGGGCTGAACTCACCGGCCGCCCGCGCGCCCggtgttt                                   >  8:22344/1‑133 (MQ=255)
                                                                                                   ccgccTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCGGGTATTTGGCCACAGCACCCCGCCCCATCAGGGTGAAGAGGCCCTGGCTGAACTCCCCGGCCGCCCGCGCGCCCTgttgttg                                  >  8:103109/1‑136 (MQ=255)
                                                                                                      ccTGGCAGGCCGGGGCCGAGGGGGGGGGTGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTcc                               <  5:89126/137‑1 (MQ=255)
                                                                                                         ggCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCg                           >  5:77099/1‑137 (MQ=255)
                                                                                                           ccggCCGGCGCCGCGCGGGGGGGGGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCgg                          <  3:229397/135‑1 (MQ=255)
                                                                                                           cAGGCCGGGGCCGCCGGGGGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCgg                          <  5:278831/137‑1 (MQ=255)
                                                                                                                           gcTGGGGTGT‑GGGGGGGGGGCCGAGCACCGGGTAGTTTGCCCCAGCCCCCCGCCCCATCAGGTTGTAGATGCACTGGCGGAACGTACCGGCCGCCCCCGCGCCCCGTTGTTATCCACCGGCTATATGGGCAAtaggg         >  6:194815/1‑133 (MQ=255)
                                                                                                                                ggggggggggggggggCCTCGCACCTGGTAGTTTGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGgcgc     <  8:243413/132‑1 (MQ=255)
                                                                                                                                   gggggggggggggCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCCAt  <  6:283860/135‑1 (MQ=255)
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AAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCCAT  >  ACB122/434243‑434510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑