Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,488,1280GT32.4% 54.1 / 14.3 38T124N (ACC→AAC) decR_2DNA‑binding transcriptional activator DecR
Reads supporting (aligned to +/- strand):  ref base G (19/6);  new base T (0/12);  total (20/18)
Fisher's exact test for biased strand distribution p-value = 1.00e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTTGCTCGGAAAGATCCGTCTCAGATATGTTTCTCCGACACCGGTATCACCCGTCTTTCCTTCACCGCTTTGTACGAAAAACTCGAATAGATCTCCTTCACCCCCGGCAACCGCTGCAGCACTTCCCGGGTAAATTCGCCAAACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTCATAGCGGCCGGAGATGTTGTGGCAGGCGACGATCTCGGGGATTTCCATCAGGCGCTGTTCAAACGCCAGCGCCATGTCCTTG  >  ACB122/1487999‑1488262
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gtTGCTCGGAAAGATCCGTCTCAGATATGTTTCTCCGACACCGGTATCACCCGTCTTTCCTTCACCGCTTTGTACGAAAAACACGAATAGATCTCCTTCACCCCCGGCAACAGCTGCAGCACTTCCCGGCTAAATTc                                                                                                                                 >  6:179438/1‑137 (MQ=255)
   gCTCGGAAAGATCCGTCTAATATATGTTTCTCCGACACCGTTATCACCGGTCTTTCCTTCACCGCTTTGTACTAAAAACTCTATTAGATCTCGTTCACCCCCGGCAACCGCTGCAGCACTTCCGGGTTAATTTCGcc                                                                                                                              <  1:63451/137‑1 (MQ=255)
            gATCCGTCTCAGATATGTTTCTCCGACACCGGTATCACCCGTCTTTCCTTCACCGCTTTGTACGAAAAACTCGAATAGATCTCCTTCACCCCCGGCAACCGCTGCAGCACTTCCCGGGTAAATTCGCCAAACGACTc                                                                                                                     >  3:36954/1‑137 (MQ=255)
                         tatTTTTCTCCGACACCGGAATCACCCGTCTTTCCTTCACCGCTTTTTACTAAAAACTCTAATAGATATCATTCACCCCCGGCAACCGCTGCAGCATTTCTTTTTTATTTTCTAAAAACGACTCCAGGTCCCGTggc                                                                                                        <  4:227892/137‑3 (MQ=255)
                         tatTTTTATCCGACCCCGGTATCACCCGTCTTTCCTTCCCCGATTTGTACTAAAAACTCTAATAGATCTCATTCACCCCCGGCACCCGCGGCAGCTGTTCGTTTTTATTTTCTAAAAACGACTCCAGGTCCCGTggc                                                                                                        <  4:231598/137‑3 (MQ=255)
                               tctcCGACACCGGTATCACCCGTCTTTCCTTCACCGCTTTGTACGAAAAACTCGAATAGATCTCCTTCACCCCCGGCAACCGCTGCAGCACTTCCCGGGTAAATTCGCCAAACGACTCCAGGTCCCGTGCCAGGAtc                                                                                                  >  3:269137/1‑137 (MQ=255)
                               tctcCGACACCGGTATCACCCGTCTTTCCTTCACCGCTTTGTACGAAAAACTCGAATAGATCTCCTTCACCCCCGGCAACCGCTGCAGCACTTCCCGGGTAAATTCGCCAAACGACTCCAGGTCCCGTGCCAGGAtc                                                                                                  >  4:156551/1‑137 (MQ=255)
                               tctcCGACACCGGTATCACCCGTCTTTCCTTCACCGCTTTGTACGAAAAACTCGAATAGATCTCCTTCACCCCCGGCAACCGCTGCAGCACTTCCCGGGTAAATTCGCCAAACGACTCCAGGTCCCGTGCCAGGAta                                                                                                  >  1:218542/1‑136 (MQ=255)
                                   cGACACCGGTATCACCCGTCTTTCCTTCCCCGCTTTGTACGAAAAACTCTAATAGATCTCCTTCACCCCCGGAACCCGCTGCAGCACTTCCCGGGTAAATTCGCCACACGACTCCAGGTCCCGTGCCAGGATCTCca                                                                                              <  6:54918/137‑1 (MQ=255)
                                     acacCGGTATCACCCGTCTTTCCTTCACCGCTTTGTACGAAAAACTCGAATAGATCTCCTTCACCCCCGGCAACCGCTGCAGCACTTCCCGGGTAAATTCGCCAAACGACTCCAGGTCCCGTGCCAGGATCTCcagc                                                                                            >  7:42946/1‑137 (MQ=255)
                                            tATCACCGGGATTTCCTTCACCTTTTTTAACTAAAAACTCTAATAGATCTCCTTCCCCCCCGCCAACGGCTGCAGCACTCCCCGGTTAAATTCGCCAAACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGt                                                                                     <  4:36954/137‑1 (MQ=255)
                                             aacACCCGTCTTTCCTTCACGGTTTTTTACAAAAAAATCTAATACATCTCCTTCCCCCCCGGCACCCGCTGCAGCACTTCCCGGTTAAATTCCCCAAACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTc                                                                                    <  6:75760/135‑1 (MQ=255)
                                             aTCCCCCGTATTGCATTCCCGGTTTTTTACTAAAAACTCTAATAGAGAACCTACCCCCCCGCCAACCGCTGCACCACTACCGGGTTAAATTCGCAAAACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTc                                                                                    <  4:196437/137‑1 (MQ=255)
                                              acACCGGTCTTTCCTTCCCCGATTTGTACTAAAAACTCTAATAGATATCCTTACCCCCCGGCAACGGCTGCAGCACTTCCGGGTTAAATCCGCCACACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTCa                                                                                   <  4:35790/136‑1 (MQ=255)
                                                    gTCTTTCCTTCACCGCTTTGTACGAAAAACTCGAATAGATCTCCTTCACCCCCGGCAACCGCTGCAGCACTTCCCGGGTAAATTCGCCAAACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTCATAGCgg                                                                             >  3:35790/1‑137 (MQ=255)
                                                        ttCCTTCACCGCTTTGTACGAAAAACTCGAATAGATCTCCTTCACCCCCGGCAACCGCTGCAGCACTTCCCGGGTAAATTCGCCAAACGACTCCAGGTTCCGTGCCAGGATCTCCAGCAGAAAGTCATAGCGGCCgg                                                                         >  1:205518/1‑137 (MQ=255)
                                                            ttCACCGCTTTGTACGAAAAACTCGAATAGATCTCCTTCACCCCCGGCAACCGCTGCAGCACTTCCCGGGTAAATTCGCCAAACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTCATAGCGGCCGGAGAt                                                                     >  1:264544/1‑137 (MQ=255)
                                                               aCCGCTTTGTACGAAAAACTCGAATAGATCTCCTTCACCCCCGGCAACCGCTGCAGCACTTCCCGGGTAAATTCGCCAAACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTCATAGCGGCCGGAGAtgtt                                                                  >  8:128730/1‑137 (MQ=255)
                                                                          cGAAAAACTCGAATAGATCTCCTTCACCCCCGGCAACCGCTGCAGCACTTCCCGGGTAAATTCGCCAAACGACTCCAGGTCCCGTGACAGGATCTCCAGCAGAAAGTCATAGCGGCCGGAGATGTTGTGGCAGGcga                                                       >  2:264708/1‑137 (MQ=255)
                                                                               aaCTCTAATAGATCTCCTTCACCCCCGGCAACCGCTGCAGCACTTCCCGGTTAAATTCCCCAAACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTCATAGCGGCCGGAGATGTTGTGGCAGGCGACGAtc                                                  <  8:96144/137‑1 (MQ=255)
                                                                                aCTCGAATAGATCTCCTTCACCCCCGGCAACCGCTGCAGCACTTCCCGGGTAAATTCGCCAAACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTCATAGCGGCCGGAGATGTTGTGGCGGGCGACGAtct                                                 >  5:117709/1‑137 (MQ=255)
                                                                                aCTCGAATAGATCTCCTTCACCCCCGGCAACCGCTGCAGCACTTCCCGGGTAAATTCGCCAAACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTCATAGCGGCCGGAGATGTTGTGGCAGGCGACGAtct                                                 >  6:197795/1‑137 (MQ=255)
                                                                                        aGATCTCCTTCACCCCCGGCAACCGCTGCAGCACTTCCCGGGTAAATTCGCCAAACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTCATAGCGGCCGGAGATGTTGTGGCAGGCGACGATCTCGGGGAtt                                         >  1:143753/1‑137 (MQ=255)
                                                                                               ctccACCCCCGGCAACCGCTGTAGCACTTCCCGGGTAAATTCGCCAAACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTCATAGCGGCCGGAGATGTTGTGGCAGGCGACGATCTCGGGGATTTCCATCa                                  >  8:58651/4‑137 (MQ=255)
                                                                                               cTTCACCCCCGGCAACCGCTGCAGCACTTCCCGGGTAAATTCGCCAAACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTCATAGCGGCCGGAGATGTTGTGTCAGGCGACGATCTAGGGGATTTCCAtaa                                  >  8:99971/1‑135 (MQ=255)
                                                                                                ttCCCCCCCGCCAACCGCTCCAGCACTTCCCGGTTAAATGCGCCACACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTCATAGCGGCCGGAGATGTTGTGGCAGGCGACGATCTCGGGGATTTCCATCAg                                 <  4:269137/137‑1 (MQ=255)
                                                                                                tacACCCCCGCCACCCGCTGCACCACTTCCCGGGTAAATTCCCCACACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTCATAGCGGCCGGAGATGTTGTGGCAGGCGACGATCTCGGGGATTTCCATCAg                                 <  8:273370/135‑1 (MQ=255)
                                                                                                  cACCCCCGGCAACCGCTGCAGCACTTCCCGGGTAAATTCGCCAAACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTCATAGCGGCCGGAGATGTTGTGGCAGGCGACGATCTCGGGGATTTCCATCAGgc                               >  2:107464/1‑137 (MQ=255)
                                                                                                       cgcccAACCGCTGCACCACTTCCGTGGTAAATTCCCCAAACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTCATAGCGGCCGGAGATGTTGTGGCAGGCGACGATCTCGGGGATTTCCATCAGGCGCTGt                          <  3:27772/133‑1 (MQ=255)
                                                                                                       ccGGCAACCGCGGCACCATTTCCCGGGTAAATTCCCAACACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTCATAGCGGCCGGAGATGTTGTGGCAGGCGACGATCTCGGGGATTTCCATCAGGCGCTGt                          <  3:69964/137‑1 (MQ=255)
                                                                                                            aaCCGCTGCAGCACTTCCCGGGTAAATTCGCCAAACGACTCCAGGTCCCGTGCGAGGATATCCAGCAGAAAGTCTTAGCGGCCGGAGATGTTGTGGCAGGCGACGATCTCGGGGATTTCCATCTGGCGCTGTTCaaa                     >  2:294202/1‑137 (MQ=255)
                                                                                                                  ggcagcaCTTCCCGGGTAATTTCGCCACACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTCATAGCGGCCGGAGATGTTGTGGCAGGCGACGATCTCGGGGATTTCCATCAGGCGCTGTTCAAACGCCAg               <  8:204932/136‑1 (MQ=255)
                                                                                                                   gcagcaCTGCCCGGTTAATTGCGCCAAACGACTCCAGGTCCCGTGCCAGGAGCTCCAGCAGAAAGTCATAGCGGCCGGAGATGTTGTGGCAGGCGACGATCTCGGGGATTTCCATCAGGCGCTGTTCAAACGCCAgc              <  4:83706/137‑1 (MQ=255)
                                                                                                                    cagcaCTTCCCGGGTAAATTCGCCAAACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTCATAGCGGCCGGAGATGTTGTGGCAGGCGACGATCTCGGGGATTTCCATCAGGCGCTGTTCAAACGCCAgcg             >  4:100088/1‑137 (MQ=255)
                                                                                                                    cagcaCTTCCCGGGTAAATTCGCCAAACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTCATAGCGGCCGGAGATGTTGTGGCAGGCGACGATCTCGGGGATTTCCATCAGGCGCTGTTCAAACGCCAgcg             <  5:197795/137‑1 (MQ=255)
                                                                                                                    cagcaCTTCCCGGGTAAATTCGCCAAACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTCATAGCGGCCGGAGATGTTGTGGCAGGCGACGATCTCGGGGATTTCCATCAGGCGCTGTTCAAACGCCAgcg             >  8:188214/1‑137 (MQ=255)
                                                                                                                            cccGGTTAAATTCGCCAAACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTCATAGCGGCCGGAGATGTTGTGGCAGGCGACGATCTCGGGGATTTCCATCAGGCGCTGTTCAAACGCCAGCGCCATGTcc     <  3:64076/137‑1 (MQ=255)
                                                                                                                             ccGGTTAAATGCGCCAAACGACTCCAGGTACCGTGACAGGATCTCCATCAGAAAGTCATAGCGGCCGGAGATGTTGTGGCAGGCGACGATCTCGGGGATTTCCATCAGGCGCTGTTCAAACGCCAGCGCCATGTCCt    <  2:100033/137‑1 (MQ=255)
                                                                                                                               gggTAAATTCGCCAAACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTCATAGCGGCCGGAGATGTTGTGGCAGGCGACGATCTCGGGGATTTCCATCAGGCGCTGTTCAAACGCCAGCGCCATGTCCTTg  >  4:245817/1‑137 (MQ=255)
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GTTGCTCGGAAAGATCCGTCTCAGATATGTTTCTCCGACACCGGTATCACCCGTCTTTCCTTCACCGCTTTGTACGAAAAACTCGAATAGATCTCCTTCACCCCCGGCAACCGCTGCAGCACTTCCCGGGTAAATTCGCCAAACGACTCCAGGTCCCGTGCCAGGATCTCCAGCAGAAAGTCATAGCGGCCGGAGATGTTGTGGCAGGCGACGATCTCGGGGATTTCCATCAGGCGCTGTTCAAACGCCAGCGCCATGTCCTTG  >  ACB122/1487999‑1488262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑