Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,440,3630AC54.5% 5.6 / 16.7 23A24A (GCA→GCC) CJ019_03266hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (5/5);  new base C (12/0);  total (18/5)
Fisher's exact test for biased strand distribution p-value = 9.57e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.97e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCAGC  >  ACB122/3440229‑3440499
                                                                                                                                      |                                                                                                                                        
tggtggTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGc                                                                                                                                        >  3:111686/1‑137 (MQ=255)
      tAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGgcgcccggggcgcccgggg                                                                                                                                  >  2:145024/1‑136 (MQ=255)
       aGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGgtg                                                                                                                                 >  3:278565/1‑137 (MQ=255)
        gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCCCGGGCGGCGCCCCGGCCGCCggggggg                                                                                                                                >  1:207579/1‑134 (MQ=255)
                           cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCTGGGGGTGGCAGGCCAACCCCGATaa                                                                                                             >  5:192920/1‑137 (MQ=255)
                            aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCTGGGTGTGGCAGGCCAACGCCTATaaa                                                                                                            >  5:78567/1‑136 (MQ=255)
                                    ctACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCAGCCAGCGCTGGCGGCGCCCTGCCAGCCTGGGTTTGGCCGGCCAAACCCAATAACTCCCAGCt                                                                                                    >  8:40885/1‑137 (MQ=255)
                                             agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGGCGGCCCACCCCGAAAACGCGCAGCGAGCTGAACa                                                                                           >  8:65110/1‑137 (MQ=255)
                                             agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCGGGGTGTGGCAGGCCACAGCCGATAAGGCGCCGCGCGCGGATCa                                                                                           >  2:24637/1‑137 (MQ=255)
                                             agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCACCCCGGGCGGCGCCCGGGCCGCCTGGGGGTGGCACGCCACCGCCGATAAGGCGCACCGCGCGGATCa                                                                                           >  8:157114/1‑137 (MQ=255)
                                                gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCCCCACCGCTGGCGGCGCCCCGGCCGCCAGGGTGTGGCAGGCCCACCCCGATAATGCGCAGCGCGCTGATCAGGc                                                                                        >  6:52412/1‑137 (MQ=255)
                                                gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCTGGGTGTGGCAGGCCAACCCCTATAAGGCGCAGCTCGCTGATCAGGc                                                                                        >  4:109801/1‑137 (MQ=255)
                                                gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGCGGCGGCCGGGCGGCCGGGGGGGGGGCGGGCAACCCCGATAAAGCGCCGCTCGCGGCTCAggg                                                                                        >  2:150497/1‑136 (MQ=255)
                                                gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCACCGCTGGCGGCGCCCGGGCAGCCTGGGGGTGGCTGGCCCACCCCGAAAAGGAGCAGCGCGCTGGTCAGGc                                                                                        >  5:31763/1‑137 (MQ=255)
                                                gcTCGAGGTGCTGCAGTGAGCGCTAGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCCCAGGCGGCCCCCGGGCCGCCAGGGTGGGGCAGGCCAACGCCCATAAGGCGCAGCGCGCTGaaaccgc                                                                                        >  6:209371/1‑131 (MQ=255)
                                                  tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCCCGCCGGCGGCGCCCCGGCCCCCGGGGGGGGGGAGGCCCCCGCCTATAAAGCGCAGCGAGCGGAGCAGGccc                                                                                      >  5:5603/1‑136 (MQ=255)
                                                  tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCCACGCCTATAAGGCGCAGCGCGCGGATCAGGccc                                                                                      >  8:133305/1‑136 (MQ=255)
                                                  tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGCCGGCCCACGCCGATAAGGCGCCGCGCGCTGGTCCGGCCg                                                                                      >  6:2947/1‑137 (MQ=255)
                                                     tggggctgcCGCGCGCGCGGGGGCCCCCCGCCTGGGCGCGGGGGCGCGGGTGCGGGCCGCCCGGGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATg                                                                                   <  8:29579/133‑1 (MQ=255)
                                                                     gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCTGGGGGTGGCAGGCCCACGCCGATAAGGCGCCGCTCGCTGATCAGGCCGCTGACTTCGGGACCCAGtt                                                                   >  4:275772/1‑137 (MQ=255)
                                                                                       ggggcggTGGGGCGGCTGCGGGCCGGGAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc                                                 <  7:186585/131‑1 (MQ=255)
                                                                                                 ggctgcggcgGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTg                                       <  3:275772/136‑1 (MQ=255)
                                                                                                                                    gCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCa    <  7:187408/137‑1 (MQ=255)
                                                                                                                                     cAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCAg   <  7:14616/137‑1 (MQ=255)
                                                                                                                                      aGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCAgc  <  6:192920/137‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                        
TGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCAGC  >  ACB122/3440229‑3440499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑