Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,688,6370AC47.9% 1.3 / 17.5 48S236R (AGC→CGC) pyrC'Dihydroorotase‑like protein
Reads supporting (aligned to +/- strand):  ref base A (7/18);  new base C (5/18);  total (12/36)
Fisher's exact test for biased strand distribution p-value = 7.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAAC‑AGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACG  >  ACB122/5688504‑5688763
                                                                                                                                      |                                                                                                                              
ggCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAAC‑AGAGCGGCGTGGGGGCGCCCTTCAGCCCGAACACCaccc                                                                                                                             >  1:102418/1‑134 (MQ=255)
         aGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAAC‑AGAGCGGCGTGCGTGCGCCCTTCAGCCAGATCACCAGCGCCCGTGgcg                                                                                                                    >  7:153135/1‑137 (MQ=255)
               cATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAAC‑AGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGc                                                                                                              >  1:11726/1‑137 (MQ=255)
                 tGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAAC‑AGAGCGGCGTGCGTGCGCACTTCACCCAGATCACCAGCGCCCGTGGCGCGCGGCTg                                                                                                            >  7:252202/1‑137 (MQ=255)
                    ccAGCTTCCTCGGCCGGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAAC‑AGAGCGGCGGGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATc                                                                                                         <  1:38564/137‑1 (MQ=255)
                          gccTCGGCCTGCCGGGCAGCCCGGAGAGCGCCGAAACCGTGGCCCGGGCGCGCACCCTGCGGCGGGTGTAAC‑AGCGCGGCGTGCGGGCGAACTGCAGCAAGATCACCCGCGCCCGGGGCGCGCGGCTGATCgcccag                                                                                                   <  7:33808/136‑1 (MQ=255)
                                gCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAAC‑AGAGCGGCGTGCGTGCGCCCGTCAGCCAGATAACCCCCGCCCCTGGCGCCCGGCTGATAgccccggcccac                                                                                             >  6:2596/1‑136 (MQ=255)
                                gCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAAC‑AGAGCGGCGTGCGGGCGGACTTCAGCCAGCTCCCCCGCTCCCGGGGCGCGCGGGGGATAgcccaggccccg                                                                                             >  8:246936/1‑135 (MQ=255)
                                    gCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAAC‑AGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGCTCGCCccggcccaggccc                                                                                         >  8:219264/1‑136 (MQ=255)
                                      ggggCATCCCGGAGAGCGCGGAAACCGGGGCCCTGGCGCGCAACGTGCTGCTGGGGCAAC‑CGCGGGGCGTGCGTGCGCACTTCCGCCAGATCACCAGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTg                                                                                       <  6:249112/136‑1 (MQ=255)
                                      cGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAAC‑AGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCGTg                                                                                       >  5:267363/1‑137 (MQ=255)
                                       gggCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAAC‑AGAGCGGCGTGCGTGGGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGGGGATAGCCCAGGCCCAGGCCTTgg                                                                                      >  7:114361/1‑137 (MQ=255)
                                               cGGAGAGCGCCGAAACCGGGGCCCTGGCGCGCAACCTGCTGCTGGTGGAAC‑AGCGCGGCGTGCGTGCGCACTTCAGCAAGATCCCCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCg                                                                              <  4:201357/137‑1 (MQ=255)
                                                    ggcgcCGAAACGGTGGGCGTGGGGCGCAACGTGCTGGGGGGGGAAG‑AGCGGGGCGGGCGGGCGCACTTCAGCAATTTCCCCAGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                         <  2:292811/136‑1 (MQ=255)
                                                    cgcgcCTAAACGGTGGCCCTGGGGCGCAACCTGCTGCTGGTGGAAC‑AGAGGGGGGGGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                         <  5:2596/136‑1 (MQ=255)
                                                      cgcCGAAACCGGGGCCCGGGCGCGCAACCTGCTGCTGGGGGACC‑AGAGCGGCGTGCGGGCGCACTTCAGCCAGATCACCCGCGCCCGTGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccg                                                                       <  6:181598/137‑1 (MQ=255)
                                                        ccGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAAC‑AGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgcc                                                                     >  5:152028/1‑137 (MQ=255)
                                                         cGAACCGGTGGCCCTGGGGCGCAACCTGCTGCGGGTGCAAC‑AGCGCGGCGGGCGTGCGAACTTCAGCCAGAACACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                    <  3:260503/137‑1 (MQ=255)
                                                         cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAAC‑AGAGCGGCGTGGGGGGGGCCTTGAGCCCGGTAACCCGCCCCCCTGGGGCGCGGGGGATCGCCCCGGCCCCCGCCGTGGGGCGGGCGGGGAccgccg                                                                    >  2:275684/1‑137 (MQ=255)
                                                         cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAAC‑AGAGCGGCGTGCGTGCGCACATCAGCCAGATCACCAGCGCCCGTGGCGCCCGGCTGATCGCCCACGCCCACGCCGTGGGCCTGCCGGTAAccgccc                                                                    >  4:260503/1‑136 (MQ=255)
                                                         cGAAACCGGGGCCCTGGCGCGCAACCGGCTGCTGGGGGAAC‑AGCGCGGCGTGCGGGCGCACTTCACCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                    <  5:62906/137‑1 (MQ=255)
                                                             aCCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAAC‑AGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGt                                                                <  3:111629/137‑1 (MQ=255)
                                                              ccggggccggggCGCGCACCCGGCGGCT‑CTGGCACAAGTGCGGCGCGCGTGCGAAGTTCAGCCCGATCCCCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                               <  1:179537/137‑1 (MQ=255)
                                                              ccGTGGCCCGGGCGCGCAACCTGCTGCTGGTGGAAC‑AGCGCGGCGTGCGTGCGCACTTCAGCCAGAGCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                               <  6:67677/137‑1 (MQ=255)
                                                              ccGTGGCCCGGGCGCGCAACCTGCTGCTGGTGGAAC‑AGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                               <  4:93331/137‑1 (MQ=255)
                                                              ccGTGCCCCTGCCTCGCAACCTGCTGCTGGTGTAAC‑AGAGCGGCGGGCGTGCTCACTTAAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                               <  8:16684/137‑1 (MQ=255)
                                                              ccGGGGCCCTGGCGCGCAACCTGCTGCTGGTGGAAC‑AGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                               <  4:48997/137‑1 (MQ=255)
                                                                   gCCGTGGCGCGCAACCTGCTGCGGGTGTAAC‑AGAGCGGCGTGCGTGCGCACTTCAGCCAGAGCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCt                                                          <  6:157794/137‑1 (MQ=255)
                                                                          ggcgcAACCTGCTGCTGGTGCAAC‑AGAGCGGCGGGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAg                                                   <  2:102418/136‑1 (MQ=255)
                                                                            cgcAACCTGCTGCTGGTGGAAC‑AGAGCGGCGTGCGTGCGCACTTCCGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCt                                                 <  8:122843/137‑1 (MQ=255)
                                                                              aaaCCTGCTGCTGGTGGAAC‑AGAGGGGCGGGAGGGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGa                                               <  7:285621/136‑1 (MQ=255)
                                                                               aaCCTGCTGGTGGTGGAAC‑AGCGCGGGGGGGGTGCGAACTTCACCCAGACCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGAt                                              <  3:168654/137‑1 (MQ=255)
                                                                                 ccTGCTGCTGTTGTAAC‑ATAGCGGCTGGCGTGCGCAGTTCAGCAAGATCACCAGCGCCGTTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATcc                                            <  7:148001/137‑1 (MQ=255)
                                                                                  ctgctgctgGTGCAAC‑AGAGCGGCGGGGGTGCGCAGTTCAGCCACATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCt                                           <  5:184359/137‑1 (MQ=255)
                                                                                          ggtgtAAC‑AGAGCGGGGTGGGGGGGCACTGCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACg                                   <  2:269768/137‑1 (MQ=255)
                                                                                            gggAAC‑AGAGCGCCTGGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAg                                 <  7:235174/136‑1 (MQ=255)
                                                                                              gAAC‑AGAGCGGCGTGCGTGCGCCCTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCCGGCCTTGGGCCTGCCGGTAACCGCCGCTGTAGCGCAGTACCAGCTGATCCGCACGGCCGAGTc                               >  5:261345/1‑137 (MQ=255)
                                                                                                     cgcggcgTGCGGGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                         <  7:219264/136‑1 (MQ=255)
                                                                                                     cgcggcgTGCGGGCGCACTTCAGCCACAGCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                         <  6:88587/136‑1 (MQ=255)
                                                                                                      ggggggtgcgtgcGAACTTCTGCCTGATCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcgc                        <  5:89576/132‑1 (MQ=255)
                                                                                                          ggtgcgtgcTCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACAGACGAGTCGCTGCGCGAGt                    <  1:242020/136‑1 (MQ=255)
                                                                                                          ggtgcgtgcGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                    <  6:152028/136‑1 (MQ=255)
                                                                                                          ggtgcgtgcGAACTTAAGCCAGATCCCCAGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                    <  2:181657/136‑1 (MQ=255)
                                                                                                          ggtgcgggcGACCTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCGGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                    <  6:164694/136‑1 (MQ=255)
                                                                                                          ggggcgggcgCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGTACTAGTCGCTGCGCGAGt                    <  1:9631/134‑1 (MQ=255)
                                                                                                              ggtgcgCACTTCCGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTtctc                <  2:60369/136‑1 (MQ=255)
                                                                                                               gtgcgCACTTCACCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc               <  2:266670/137‑1 (MQ=255)
                                                                                                                 gcgcACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAg             <  8:252202/137‑1 (MQ=255)
                                                                                                                            ccAGATCACCCGCCCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACg  <  5:271119/137‑1 (MQ=255)
                                                                                                                                      |                                                                                                                              
GGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAAC‑AGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACG  >  ACB122/5688504‑5688763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑