Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 539,416 G→C 6.3% S65S (TCG→TCC)  rpsL → 30S ribosomal protein S12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122539,4160GC 6.3% 207.7 / 6.2 64S65S (TCG→TCC) rpsL30S ribosomal protein S12
Reads supporting (aligned to +/- strand):  ref base G (23/37);  new base C (2/2);  total (25/39)
Fisher's exact test for biased strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.99e-01

TTCCTGCGCTGCAGAACTGCCCGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGCGTTAAAGG  >  ACB122/539283‑539547
                                                                                                                                     |                                                                                                                                   
ttCCTGCGCTGCAGAACTGCCCGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTa                                                                                                                                   <  4:91264/136‑1 (MQ=255)
        cTGCAGAACTGCCCGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCggtg                                                                                                                           <  4:335299/136‑1 (MQ=255)
        cTGCAGAACTGCCCGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCggtg                                                                                                                           <  4:340926/136‑1 (MQ=255)
        cTGCAGAACTGCCCGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCggtg                                                                                                                           <  4:360730/136‑1 (MQ=255)
               aCTGCCCGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAgg                                                                                                                    <  7:280742/136‑1 (MQ=255)
                cTGCCCGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAgg                                                                                                                    <  2:314443/135‑1 (MQ=255)
                   cccGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCCCGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGGGAAGGCcac                                                                                                                >  7:312506/1‑136 (MQ=255)
                   cccGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCggtg                                                                                                                           >  8:227740/1‑125 (MQ=255)
                   cccGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCggtg                                                                                                                           <  7:227740/125‑1 (MQ=255)
                    ccGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGcc                                                                                                                  <  1:114690/133‑1 (MQ=255)
                      gCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACaa                                                                                                              >  6:392215/1‑135 (MQ=255)
                          cgtcgtGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTg                                                                                                          <  2:360512/135‑1 (MQ=255)
                          cgtcgtGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTg                                                                                                          <  1:97115/135‑1 (MQ=255)
                               tGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAaga                                                                                                     <  5:351571/135‑1 (MQ=255)
                               tGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAaga                                                                                                     <  5:392215/135‑1 (MQ=255)
                                ggCGTGTGCACCCGTGTGTACACCACCACGCCGCAAAAACCAAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGCCCAACGGTTTAGAGGTTTCCTCGTACATCGGTGGTGCAGGCCCCCACCTGCAagag                                                                                                    >  4:374922/1‑135 (MQ=255)
                                  cGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTACTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGca                                                                                                  >  8:68097/1‑135 (MQ=255)
                                   gtgtGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGcaca                                                                                                <  7:390272/136‑1 (MQ=255)
                                         ccccGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGGTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGcgtc                                                                                           >  1:13096/2‑135 (MQ=255)
                                         aCCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGcgtcg                                                                                          >  3:202670/1‑136 (MQ=255)
                                               gtgtACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTg                                                                                     <  1:98153/135‑1 (MQ=255)
                                               gtgtACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTg                                                                                     <  2:191166/135‑1 (MQ=255)
                                                 gtACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAgg                                                                                                                    >  6:81520/1‑102 (MQ=255)
                                                 gtACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAgg                                                                                                                    >  6:75696/1‑102 (MQ=255)
                                                 gtACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAgg                                                                                                                    <  5:81520/102‑1 (MQ=255)
                                                 gtACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAgg                                                                                                                    <  5:75696/102‑1 (MQ=255)
                                                       caccacGCCTAATAAAGCTATCTCGGCACTGCGTAAAGTTTGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCTGTGGTGAAGGCCACAACCTTCAAGAGCACAGCGTCGTCCTGATTCGTgg                                                                             <  6:131098/135‑1 (MQ=255)
                                                       caccacGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTgg                                                                             <  2:13096/135‑1 (MQ=255)
                                                        accacGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTggcg                                                                           <  7:56349/136‑1 (MQ=255)
                                                        accacGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTggc                                                                            >  1:60564/1‑135 (MQ=255)
                                                          cacTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGCCGTGTTCGTCTGACTAACGGTTTCGAAGTGACTTCCTACATCGGTGGTGAAGGTCACAACCTGCAgga                                                                                                     >  2:18966/1‑108 (MQ=255)
                                                          cacTCCTAAAAAACCGAACTCCGCGCTGCGTAAAGTATGCCGTGTTCGTCTGACTAACGGTTTCGAAGTGACTTCCTACATCGGTGGTGAAGGTCACAACCTGCAgga                                                                                                     <  1:18966/108‑1 (MQ=255)
                                                             gCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCgt                                                                      >  7:334628/1‑136 (MQ=255)
                                                             gCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCg                                                                       >  6:343266/1‑135 (MQ=255)
                                                               cGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTGGAGGTTTCCTCGTa                                                                                                                                   <  5:244761/73‑1 (MQ=255)
                                                               cGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTa                                                                                                                                   >  6:244761/1‑73 (MQ=255)
                                                               cGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCgt                                                                      >  2:240521/1‑134 (MQ=255)
                                                                  aaaaaCCGAACTCCGCGCTGCGTAAAGTATGCCGTGTTCGTCTGACTAACGGTTTCGAAGTGACTTCCTACATCGGTGGTGAAGGTCACAACCTGCAGGAGCACTCCGTGATCCTGATCCGTGGCGGTCgtgtt                                                                   >  6:9047/1‑133 (MQ=255)
                                                                   aaaaCCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTaaa                                                                 <  5:343266/135‑1 (MQ=255)
                                                                   aaaaCCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTaaa                                                                 <  5:269369/135‑1 (MQ=255)
                                                                    aaaCCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGAATCGTGGCGGCCGTGTaa                                                                  >  1:18906/1‑133 (MQ=255)
                                                                         tAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGACACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACtt                                                           <  5:80678/135‑1 (MQ=255)
                                                                               ggCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGgt                                                    <  3:235240/136‑1 (MQ=255)
                                                                               ggCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGgt                                                    <  3:262613/136‑1 (MQ=255)
                                                                                         aaaGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAgg                                                                                                                    <  4:230407/62‑1 (MQ=255)
                                                                                         aaaGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAgg                                                                                                                    >  3:230407/1‑62 (MQ=255)
                                                                                         aaaGAATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACTTCGTTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTTCTTGCGCGCGTGTAAAAGTCTTGCACGGTGttggggtc                                           >  1:168216/1‑129 (MQ=255)
                                                                                              aTGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACAcc                                     >  8:55613/1‑136 (MQ=255)
                                                                                                   gtgtTCGTCTGACTAACGGTTTCGAAGTGACTTCCTACATCGGTGGTGAAGGTCACAACCTGCAGGAGCACTCCGTGATCCTGATCCGTGGCGGTCGTGTTAAAGACCTCCCGGGTGTTCGTTACCACACCGtac                                 <  5:9047/135‑3 (MQ=255)
                                                                                                   gtgtGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACACCGTTc                                 <  1:240521/135‑1 (MQ=255)
                                                                                                        cGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTCCCACACCGTTCGCGGc                            >  6:272390/1‑135 (MQ=255)
                                                                                                        cGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACACCGTTCGCGGct                           >  4:348209/1‑136 (MQ=255)
                                                                                                         gTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTACTTATTCGTGGAGGCCGTGTAAAATACTTGCAAGGTGGTCGTTACCACACCGTTCGCGGc                            <  2:26476/134‑1 (MQ=255)
                                                                                                          tCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACACCGTTCGCGGctct                         >  3:385594/1‑136 (MQ=255)
                                                                                                                  aCGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATAc                 <  4:202670/136‑1 (MQ=255)
                                                                                                                      tttCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTgg                                                                             <  2:205223/72‑1 (MQ=255)
                                                                                                                      tttCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTgg                                                                             >  1:205223/1‑72 (MQ=255)
                                                                                                                        tCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCgg            <  8:96719/135‑1 (MQ=255)
                                                                                                                           aGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTTAAAGACTTGCCAGGTGTTCGTTAACACACCGTTAGCGGGTCTCTGTATACTTCgggggg        >  7:240396/1‑133 (MQ=255)
                                                                                                                           aGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGCGt        <  8:351326/136‑1 (MQ=255)
                                                                                                                                 ccTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGCGTTAAAg   <  4:38675/135‑1 (MQ=255)
                                                                                                                                 ccTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGCGTTAAAg   <  3:348209/135‑1 (MQ=255)
                                                                                                                                 ccTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGGGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGCGTTAAAg   <  4:54822/135‑1 (MQ=255)
                                                                                                                                  cTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGCGTTAAAgg  <  7:68097/135‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                   
TTCCTGCGCTGCAGAACTGCCCGCAGCGTCGTGGCGTGTGCACCCGTGTGTACACCACCACGCCGAAAAAACCTAACTCGGCACTGCGTAAAGTATGCCGTGTGCGTCTGACCAACGGTTTCGAGGTTTCCTCGTACATCGGTGGTGAAGGCCACAACCTGCAAGAGCACAGCGTCGTCCTGATTCGTGGCGGCCGTGTAAAAGACTTGCCAGGTGTTCGTTACCACACCGTTCGCGGCTCTCTGGATACTTCGGGCGTTAAAGG  >  ACB122/539283‑539547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑