Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | ACB122 | 1,523,480 | T→G | 22.2% | P46P (CCT→CCG) | lpxC → | UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB122 | 1,523,480 | 0 | T | G | 22.2% | 89.5 / 3.1 | 61 | P46P (CCT→CCG) | lpxC | UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase |
Reads supporting (aligned to +/- strand): ref base T (18/22); new base G (9/6); total (33/28) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.75e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCATGGCGGGTCTGGGCATCGA > ACB122/1523347‑1523608 | ttAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCtgt < 3:190007/136‑1 (MQ=255) cccTGAAGAATACCATCCGTGCCACATGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGAACTCGCCCCAGTGGGGGAGAtgc > 6:375511/1‑133 (MQ=255) agaaTACCATCCGTGCCCCAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTgcg < 2:23464/134‑1 (MQ=255) cATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGCCACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCCGAGGTCGAGCTCCCTGCACGTGCGGc > 6:62060/1‑135 (MQ=255) aTCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCTACTTCCCCCCCGCCGACCTCGACCCAGTGGTCCAGCTCCCAGCGCGGGCGcccc > 8:89274/1‑135 (MQ=255) aTCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGCCCTCGCCCCTGTGGTCGAGATCCCTGCGCGTGCGGCCa > 3:336820/1‑136 (MQ=255) aTCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGCCCCTGTGGTCGAGATCCCTGCGCGTGCGGCCa > 4:335031/1‑136 (MQ=255) aTCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCACGACCCGCTGGTCGAGATCCCTGCGCGGGCGGCCa > 7:188195/1‑136 (MQ=255) gTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTCCCTGAACCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCCCGCCGACCTCGCCCCGGTGGTCGCTATTCCGGCGCGTTCGGccccac > 2:132563/1‑131 (MQ=255) gTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGGCGCCCTCGCCCCGGGGGGGGGGAGCCCTGGGCGTGCGGcccccc > 1:367212/1‑131 (MQ=255) gTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGCCCTCCCCCCTGGGGTCGAGCTCCCGGGGCGTGCGGCCCACg > 5:176364/1‑135 (MQ=255) tGCCACAGGTGTCGGGCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGt < 6:237424/135‑1 (MQ=255) tgtCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGCCCTCGACCCTGTGGTCGAGCTCCCTGCGCGTGCGGCCAACGTCGGCGAGCCa > 4:285120/1‑136 (MQ=255) tgtCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGCCCACGCCCCGGTGGGCGAGCACCCTGCGCGTGCGGCCCACCTCGGCgggcc > 1:288350/1‑131 (MQ=255) gTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGCCCACGCCCCTGTGGTCGCGCTCCCTGCGCGTGCGGCCCACGTCGGCGAGGCACCCAt > 8:154719/1‑136 (MQ=255) cTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGt < 7:166016/136‑1 (MQ=255) cTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTcgacg < 8:121976/136‑1 (MQ=255) cTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTcgacg < 8:116573/136‑1 (MQ=255) cTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTcgacg < 8:244314/136‑1 (MQ=255) tCCGGGGAGAAGGTTTACCTGCCCCTCACCCCTGCACCTGTTGCCCCCGGCATCGTCGTCCGCCGCGCCGACCTCGACCCGGTGGGCGAGATCCCTGGGCGCGCGGCCAACGTCGGCGAGACAACCATGTcgacg < 6:363770/135‑1 (MQ=255) cGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCACCCCCCAGTGGGGGAGCTACCTGCGCGTGCGGCCCAACTCGGCGCGCCAACCCAGTAgacgac > 6:239810/1‑135 (MQ=255) ggAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGCCCCTGTGGTCGAGCTCCCGGCGCGTGCGGCCAACGTCGGCGAGACAACCCTGTCGACGACGCt > 2:269428/1‑135 (MQ=255) gaAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCCCGCCGACCAACACCCTGTGGTCGAGATCCCTGCGCGTGGGGCCAACCTCGGCGCGACAACCAAGTCGACGACGCAggg > 5:315493/1‑134 (MQ=255) gTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAAc < 6:207027/135‑1 (MQ=255) tACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGCCCGCGCCCCGGGGGTGGAGGTCCCTGCGCGTGGGGCCCACCTCGGCGCGACCACCAGTTCGACGCCGCGGGGCCACggg > 2:359698/1‑134 (MQ=255) tACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCAGCCGCCCCGCCCACGCCCCTGTTGTCGAGATACCGGCGCCTGCGGCCAACGTAGGCGAGCCAACCAAGTCGACGCCGCTGGTaaaagct > 6:144702/1‑131 (MQ=255) tGACCCCCACGCCGCCACGTGTGGACACCGGCTGCGTCTCCCGCCGCGCCTACCTCGACCCGGGGTGCGAGATCCCTGCGCGTGGGGCCAACGGCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACg < 5:62060/135‑1 (MQ=255) cccTCACGCCTGCACCTGTTGACACCGGCAGCGGCTGCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCaa < 3:261546/136‑1 (MQ=255) cTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGCCCCGGTGGTGGCGATCCCTGCGCGGGCGGCCCACCTCGGCGCGCCCACCCAGTCGACGACGCGGGTCCACGGGGGCGGCAAg > 5:295861/1‑135 (MQ=255) cTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCAGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAgg > 3:57213/1‑136 (MQ=255) aaGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCg < 7:340403/136‑1 (MQ=255) aaGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCg < 3:204079/136‑1 (MQ=255) aaGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGGGCGGCCAACGTCGGCGAGACAACCATGGCGACGACGCTGGTCAACGGTGACGTCAAGGTCg < 7:96219/136‑1 (MQ=255) aGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCg < 5:290661/135‑1 (MQ=255) cTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGCCCCGGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTc > 4:239844/1‑134 (MQ=255) cTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATACCTGCGCGGGCGGCCCACGTCGGCGCGACAACCATGTCGACGCCGCTGGTCAACGGTGACGTGAAGGTGGATACGGt > 1:1333/1‑133 (MQ=255) ttGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAg < 4:80331/134‑1 (MQ=255) tggcccccGGCAGCGGCTTCCCCCGCCCCGCCCACCCCCGGGGGGGCGTGATCCCGGCGCGGGCGGCCAACGGCGGCGAGACAACCATGCCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGc < 6:45277/129‑1 (MQ=255) acacCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCt > 7:374880/1‑136 (MQ=255) gCAGCGTGTCCCCCCGCGCGCACCGCGACCCGGTGGCCTAGATCCCTGCGCGTGCGGCAACCGCCGGCCCCCAAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCtctc < 8:155318/136‑1 (MQ=255) cagcGTCTGCCGCCGCGCCGACCTCGACCCTGTGGGCGAGATCCCTGCGCGTGCGGCCAACGGCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCtctc < 5:42858/132‑1 (MQ=255) cATCGTCTTCCGCCGCGCCGACCTCGCCCCAGTGGTCGGGATACCTGCGCGTGCGGCCAACGTCGGCGAGACAACCAAGTAGACGCCCCTGGGCAAAGGTGTCGTCAAGGTCGAAACGGTCGCGCCACTGCtctc > 2:95518/1‑135 (MQ=255) cTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCAt < 6:315493/135‑1 (MQ=255) ccgcgccgACCTCGCCCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACCTAGGCCAGCCAACCATGTCGACGCAGCTGGTCAACGGTGACGGCAAGGTAGATACGGTCGAGCACCTGCTCTCCCCCATGGCggg > 6:199219/1‑135 (MQ=255) ccgcgccgACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCg > 7:119662/1‑101 (MQ=255) ccgcgccgACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCg < 8:119662/101‑1 (MQ=255) cgcgccgACCTCGACCCGGTGGTCGAGATCCCTGCGCGGGCGGCCAACGTCGGCGAGACAACCATGGCGCCGACGCGGGGCCACGGGGACGTCAATGTCGCTACGGTCGCGCACCTGCTCTCCGCCAAGGCggggc > 8:3761/1‑134 (MQ=255) gcgccgACCTCGACCCTGTGGTCGAGATCCCGGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCATGGCGGGTCt < 8:48947/136‑1 (MQ=255) gccgACCTCGACCCTGGGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCATGGCGGGTCTgg < 3:30527/136‑1 (MQ=255) cgACCTCGCCCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCGGGTCAACGGTGACGTCAAGGTCGATACGGTCGCGCACCGGCTCTCCGCCATGGCGGGGCTGGGc > 7:41642/1‑136 (MQ=255) cgACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGCCGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCATGGCGGGGCTGGGc > 3:225524/1‑136 (MQ=255) cgACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCt < 1:323047/111‑1 (MQ=255) cgACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCt > 2:323047/1‑111 (MQ=255) cgACCTCGACCCTGTGGTCGAGATCCCGGCGCGTGCGGCCAACGACGGCGAGACAACCATGTCGACGACGCTGGGCGACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCAGGGCGGGGCTggg > 5:167659/1‑135 (MQ=255) cgACCTCGACCCGGTGGTCGCGCTCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCAAGTCCACGACGCAGGTCAACGGTTCCGTCAACGTCGATACCGTCGAGCACCTGCTCTCCGCCATGGCGGGTCttg > 2:284384/1‑132 (MQ=255) ccTCGACCCTGTGGTGGAGATCCCTGGGCGTGCGGCCAACGTCGGCGAGACAACCTTGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCATGGCGGGTCTGGGCATc < 7:181200/136‑1 (MQ=255) cTCGCCCCGGGGGGCGAGAGCCCTGCGGGTGCGGCCACCGTCGGCTAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCATGGCGGGTCTGGGCATc < 5:239810/135‑1 (MQ=255) cTCGAGCCGGTGGTCGAGATCCGTGTGCGTGCGGAGAAGGTGGGCGAGACAACCATGGCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATTCGGTCGAGCACCTGCTCTCCGCCATGGCGGGTCTGGGCATc < 1:284384/135‑1 (MQ=255) cTCGACCCTGTGGTCGAGATCCCTGCGCGTGGGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCATGGCGGGTCTGGGCATc < 1:16230/135‑1 (MQ=255) cTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCATGGCGGGTCTGGGCATc < 6:64755/135‑1 (MQ=255) tCGCCCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCATGGCGGGTCTGGGCATCGa > 3:115965/1‑136 (MQ=255) tCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCCATACGGTCGAGCACCAGCTCTCCGCCATGGCGGGTATGGGCCTCg > 4:64256/1‑135 (MQ=255) | TTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTTGACACCGGCATCGTCTTCCGCCGCGCCGACCTCGACCCTGTGGTCGAGATCCCTGCGCGTGCGGCCAACGTCGGCGAGACAACCATGTCGACGACGCTGGTCAACGGTGACGTCAAGGTCGATACGGTCGAGCACCTGCTCTCCGCCATGGCGGGTCTGGGCATCGA > ACB122/1523347‑1523608 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |