Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 4,271,001 T→C 6.1% L196P (CTG→CCG)  gabR_1 → HTH‑type transcriptional regulatory proteinGabR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,271,0010TC 6.1% 207.6 / 3.2 65L196P (CTG→CCG) gabR_1HTH‑type transcriptional regulatory proteinGabR
Reads supporting (aligned to +/- strand):  ref base T (29/32);  new base C (2/2);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

GCCAATTCCCGGCCCAGGGCTTGCCGGAACTGCGCGAAGCCATTGCCCACCACATCGCCTACTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGTGCACGCCTGCAATCGGTGCCGGTGGATGATGAGGGCC  >  ACB122/4270872‑4271129
                                                                                                                                 |                                                                                                                                
gcCAATTCCCGGCCCAGGGCTTGCTGGAACTGCGCGAAGCCATTGCCCACCACATCGCCTACTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCCGATCGc                                                                                                                            >  7:183231/1‑136 (MQ=255)
gcCAATTCCCGGCCCAGGGCTTGCCGGAACTGCGCGAAGCCATTGCTCACCACATCGCCTACTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCg                                                                                                                             <  6:199478/135‑1 (MQ=255)
gcCAATTCCCGGCCCAGGGCTTGCCGGAACTGCGCGAAGCCATTGCCCACCACATCGCCTACTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGc                                                                                                                            >  3:343363/1‑136 (MQ=255)
 ccAATTCCCGGCCCAGGGCTTGCCGGAACTGCGCGAAGCCATTGCCCACCACATCGCCTACTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGcc                                                                                                                           >  3:97344/1‑136 (MQ=255)
   aaTTCCCGGCCCAGGGCTTGCCGGAACTGCGCGAAGCCATTGCCCACCACATCGCCTACTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCg                                                                                                                         >  4:27871/1‑136 (MQ=255)
     ttCCCGGCCCAGGGCTTGCCGGAACTCCGCGAAGCCATTGCCCACCACATCGCCTACTCGCGCGGGGGGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCgg                                                                                                                        <  1:156590/135‑1 (MQ=255)
          ggCCCAGGGCTGGCCGGAACTGCGCGAAGCCATTGCCCACCACATCGCCTACTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCt                                                                                                                   <  6:287360/135‑1 (MQ=255)
             ccAGGGCTTGCCGGAACTGCGCGAAGCCATTGCCCACCACATCGCCTACTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGt                                                                                                                >  2:293277/1‑135 (MQ=255)
             ccAGGGCTTGCCGGAACTGCGCGAAGCCATTGCCCACCACATCGCCTACTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTc                                                                                                               >  3:338711/1‑136 (MQ=255)
              cAGGGCTTGCCGGAACTGCGCGATGCCATTGCCCACCAGATCGGCTCCTCGCTGGGGGTGCAGTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTTGACCTGATCGCCCGGGTGCTGGTc                                                                                                               <  5:285908/135‑1 (MQ=255)
              cAGGGCTTGCCGGAACTGCGCGAAGCCATTGCCCACCACATCGCCTACTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTc                                                                                                               <  5:71010/135‑1 (MQ=255)
                         ggAACTGCGCGAAGCCATTGCCCACCACATCGCCTACTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGc                                                                                                   >  8:387474/1‑136 (MQ=255)
                         ggAACTGCGCGAAGCCATTGCCCACCACATCGCCTACTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGc                                                                                                   >  7:209783/1‑136 (MQ=255)
                          gAACTGCGCGAAGCCATTGCCCACCACATCGCCTACTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGc                                                                                                   <  1:162687/135‑1 (MQ=255)
                                   gAAGCCATTGCCCACCACATCGCCTACTCGCGCGGGGTGCAGTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTg                                                                                             <  5:195624/132‑1 (MQ=255)
                                   gAAGCCATTGCCCACCACATCGCCTACTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCACCGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGcc                                                                                          >  5:339401/1‑135 (MQ=255)
                                   gAAGCCATTGCCCACCACATCGCCTACTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTg                                                                                             >  6:195624/1‑132 (MQ=255)
                                   gAAGCCATTGCCCACCACATCGCCTACTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGcc                                                                                          >  5:138237/1‑135 (MQ=255)
                                   gAAGCCATTGCCCACCACATCGCCTACTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCa                                                                                         >  3:62762/1‑136 (MQ=255)
                                   gAAGCCATTGCCCACCACATCGCCTACTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCa                                                                                         >  4:84651/1‑136 (MQ=255)
                                     aGCCATTGCCCACCACATCGCCTACTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCAt                                                                                        <  1:319649/135‑1 (MQ=255)
                                             cccACCACATCGCCTACTCGCGCGGGGTGCACTGCAGTGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATcc                                                                               >  7:305463/1‑136 (MQ=255)
                                                cccacCTCGCCTCCTCGCGCGGGGTGCACTGCAGCGCCGCCGACCTGCGGGTGTGCAACGGCGCCCAGCAGGGGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTgg                                                                            <  4:278634/135‑1 (MQ=255)
                                                          cTACTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACccgccg                                                                  >  7:113240/1‑136 (MQ=255)
                                                            aCTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCcgg                                                                 >  8:182097/1‑135 (MQ=255)
                                                            aCTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCcgg                                                                 >  2:352657/1‑135 (MQ=255)
                                                             cTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCcgg                                                                 <  3:79377/134‑1 (MQ=255)
                                                               cgcgcgGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCcggcacg                                                             >  3:284600/1‑136 (MQ=255)
                                                                      ggTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTg                                                      >  3:43776/1‑136 (MQ=255)
                                                                            cTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTg                                                <  8:369904/136‑1 (MQ=255)
                                                                                   gcagcCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTgg                                         <  3:192333/136‑1 (MQ=255)
                                                                                   gcagcCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTg                                          >  6:21290/1‑135 (MQ=255)
                                                                                   gcagcCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTg                                          >  6:109376/1‑135 (MQ=255)
                                                                                    cagcCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGt                                                     >  7:223671/1‑123 (MQ=255)
                                                                                    cagcCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGt                                                     <  8:223671/123‑1 (MQ=255)
                                                                                     agcCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTgg                                                                            >  7:164719/1‑99 (MQ=255)
                                                                                     agcCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTgg                                                                            <  8:164719/99‑1 (MQ=255)
                                                                                     agcCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGt                                       <  3:27871/136‑1 (MQ=255)
                                                                                     agcCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCCGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGt                                       >  3:300512/1‑136 (MQ=255)
                                                                                      gcCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGt                                       <  6:138237/135‑1 (MQ=255)
                                                                                      gcCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCCGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGTg                                      <  8:183231/136‑1 (MQ=255)
                                                                                       ccGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTgg                                         <  1:18938/132‑1 (MQ=255)
                                                                                       ccGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTgg                                         >  2:18938/1‑132 (MQ=255)
                                                                                        cGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGTGc                                     >  6:142348/1‑135 (MQ=255)
                                                                                            ctgctgGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGTGCACGc                                 <  2:102287/135‑1 (MQ=255)
                                                                                               ctgGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGTGCACGCCTGc                             <  7:182097/136‑1 (MQ=255)
                                                                                                         aCGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGTGCACGCCTGCAATCGGTGc                    <  1:352657/135‑1 (MQ=255)
                                                                                                          cGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCCCGGCAATTGTTCCTGGCCCTGGGTGCACGCCTGCAATCGGTGCCg                  >  3:328009/1‑136 (MQ=255)
                                                                                                          cGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGTGCACGCCTGCAATCGGTGca                   >  5:333571/1‑134 (MQ=255)
                                                                                                          cGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGTGCACGCCTGCAATCGGTGCCg                  <  8:382319/136‑1 (MQ=255)
                                                                                                            gcgcCCAGCAGGCGCTGGACCTGATCGGCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGTGCACGCCTGCAATCGGTGCCGGt                <  7:77713/136‑1 (MQ=255)
                                                                                                            gcgcCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGTGCACGCCTGCAATCGGTGCCGGt                <  7:192292/136‑1 (MQ=255)
                                                                                                            gcgcCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGTGCACGCCTGCAATCGGTGCCGGt                <  4:338711/136‑1 (MQ=255)
                                                                                                            gcgcCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGTGCACGCCTGCAATCGGTGCCGGt                >  4:359583/1‑136 (MQ=255)
                                                                                                            gcgcCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAAGTGTTCCTGGCCCTGGGTGCACGCCTGCAATCGGTGCCgg                 >  6:344882/1‑135 (MQ=255)
                                                                                                            gcgcCCAGCAGGCGCTGGACCCGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGTGCACGCCTGCAATCGGTGCCGGt                <  4:300512/136‑1 (MQ=255)
                                                                                                            gcccccAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGACCTGGGTGCACGCCTGCAATCGGTGCCGGt                <  3:103695/133‑1 (MQ=255)
                                                                                                             cgcCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGTGCACGCCTGCAATCGGTGCCGGt                <  1:284565/135‑1 (MQ=255)
                                                                                                             cgcCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGTGCACGCCTGCAATCGGTGCCGGt                <  1:268505/135‑1 (MQ=255)
                                                                                                             cgcCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGTGCACGCCTGCAATCGGTGCCGGt                <  2:170708/135‑1 (MQ=255)
                                                                                                             cgcCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGTGCACGCCGGCAATCGGTGCCGGt                <  6:376334/135‑1 (MQ=255)
                                                                                                                     agGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGTGCACGCCTGCAATCGGTGCCGGTGGAtgatga       <  4:284600/136‑1 (MQ=255)
                                                                                                                       gcgcTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGTGCACGCCTGCAATCGGTGCCGGTGGATGATGAg      <  2:76002/135‑1 (MQ=255)
                                                                                                                         gcTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGTGCACGCCTGCAATCGGTGCCGGTGGATGATGAggg    <  6:188471/135‑1 (MQ=255)
                                                                                                                          cTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGTGCACGCCTGCAATCGGTGCCGGTGGATGATGAGGGcc  >  7:7442/1‑136 (MQ=255)
                                                                                                                                 |                                                                                                                                
GCCAATTCCCGGCCCAGGGCTTGCCGGAACTGCGCGAAGCCATTGCCCACCACATCGCCTACTCGCGCGGGGTGCACTGCAGCGCAGCCGACCTGCTGGTGTGCAACGGCGCCCAGCAGGCGCTGGACCTGATCGCCCGGGTGCTGGTCGAACCGGGCTGCCAGGTGGCCATGGAAGATCCTGGCTACCCGCCGGCACGGCAATTGTTCCTGGCCCTGGGTGCACGCCTGCAATCGGTGCCGGTGGATGATGAGGGCC  >  ACB122/4270872‑4271129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: