| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,288,041 | 0 | T | G | 52.4% | 8.2 / 17.6 | 21 | intergenic (+4/+66) | slyB/nhaA_1 | Outer membrane lipoprotein SlyB/Na( )/H( ) antiporter NhaA |
| Reads supporting (aligned to +/- strand): ref base T (5/5); new base G (11/0); total (16/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.24e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCAGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACG > ACB122/1287936‑1288174 | aTGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGGTTCGCGCGGGCCGGTGTGGAGTAATAAAAAAACCCGCCAGGgc > 6:206097/1‑135 (MQ=255) gcgcgcCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGGACGGGCCGGAGTGGGGTAATTAAAAAAACCGACAAGgctg > 1:382232/1‑135 (MQ=255) gcgcCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGTCCGGACTGGAGTAAAAAAAAAAACCCACAAGgctgct > 7:90052/1‑135 (MQ=255) gCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGGTTAGCACTCCCCGGACTGGAGTAAAAAAAAAACCCCACAAGGCGGGGGCCACTCggg > 3:381003/1‑135 (MQ=255) cAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTGGCCCTGACCGGAGTGCAGTAATAAAAAACCCCCCCAAGGCTGCTGCCAGTCGGGGGGTTTTGAt > 3:92254/1‑135 (MQ=255) aGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGGTTCGCACTGACCGGAGTGCAGTAATAAAAAAACCCGCCAAGGCTGCGGCCAGTCGGGGGTTTTTGAtt > 5:158867/1‑134 (MQ=255) aGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGGTTCGCACTGACCGGAGTGCAGTAATAAAAAAACCCCCCAAGGCGGCTGGCCCTCGGGGGTTTTTTAtt > 6:291221/1‑134 (MQ=255) ggAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCCCTGCCCGGAGTGCAGGAATAAAAAACCCCCACATGGCTGCTGCCAGGCGGGGGTTTTTAATgg > 6:83622/1‑134 (MQ=255) gAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTTTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCAGTCGGGGTTTTTTCATGCCTCAGcc < 2:115159/135‑1 (MQ=255) gAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCAATGAACGTAGTGGAGTAAAAAAAAAACCCGCCATTGCCGCTGCCACTAGGGGTTTTTTAAATCCTCAga > 7:72301/1‑133 (MQ=255) tCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGGAGTGGAGTAATAAAAAAACCCGACAATGCTGCTGCCAGTCGGGGTTTTTTCATTCCGCAGCCAAGTCAg > 4:252746/1‑135 (MQ=255) tCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGGTTGGCACTGGCCGGAGTGGAGTAATAAAAAAACCCCCCCAGGCTGCGGCCAGGGGGGGGGTTTTTATTGCTCAGCCaacatag > 4:203343/1‑130 (MQ=255) cTGTCGACGGTACCCGGCGCGTGTCGCACTGACCGTAGTGCATTAAAAAAAAACCCCGACATGGCTGCTGCCAGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCg < 1:349076/135‑1 (MQ=255) aCGGTACCAGTCGCGGTTAGCACTGCCCGTAGTGCAGTAATAAAAAAACCCGACAAGGCTGCTGCCAGTCGGGGTTTTTTGATTCCTCACCCAAGTCAGGCAATGCTGGGTTTGCGGGTGGCCATTGCCGTTAcc > 8:209221/1‑134 (MQ=255) ggTACCAGTCGCGTTTCGCACTGACCGGAGTGGAGTAATAAAAAACCCCGCCATGGCTGCTGCCAGTCGGGGGTTTTTCATGCCTCACCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCCTTGCGGTggcggc > 7:319245/1‑135 (MQ=255) gcgTTTAGCACGGACCGTAGTGCAGTAATAAAAAACCCCGACAAGGCTGCTGGCAGTCGGGGGTTTTTAATGCCTCAGCCAAGTCAGGCAATTCTGGTTTTTCGGCTGGCCCATTCCGTGACCGCGGTGCCGCTc > 2:10577/1‑135 (MQ=255) cgTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCGGCCAGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCa < 8:65020/135‑1 (MQ=255) cggTAGGGCAGTAATAAAAAACCCCGACATGGCTGCTGCCAGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAga < 8:72301/132‑1 (MQ=255) ccGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCAGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAga < 6:307158/134‑1 (MQ=255) gTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCAGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAgaac > 8:38397/1‑134 (MQ=255) gTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCAGTCGGGGTTTTTTACTGGCTCAGCCAAGTCAGGTAATGCTGGTTTTGCGGCTGGCCATTGCGGTGCCGGGGGTGCCGGTCGCCGCAGGCAAgcacg > 3:64799/1‑135 (MQ=255) | ATGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCAGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACG > ACB122/1287936‑1288174 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |