Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,524,7280TG43.6% 22.1 / 12.7 39P68P (CCA→CCC) CJ019_01439hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (11/11);  new base G (0/17);  total (11/28)
Fisher's exact test for biased strand distribution p-value = 6.84e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGACGCCACATGGCAGTGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCT  >  ACB122/1524596‑1524857
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ggACGCCACATGGCAGTGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTgg                                                                                                                                 >  1:191020/1‑135 (MQ=255)
  aCGCCACATGGCAGGGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGGTGAAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCTGCAGGGGGGAAGGTGGCt                                                                                                                               <  6:337972/135‑1 (MQ=255)
   cGCCACATGGCAGTGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTg                                                                                                                              >  7:316985/1‑135 (MQ=255)
         aTGGCCGGGCGCGCGGGCAGCCGGTGGCAACTGGCTTGCCAGCAGGCGCGGCATGGGCTCCAGGCGCGCGGCGTGGTTCAGCAGCAGGCGCCGCGGGCGCACGTGGCGCAGCCGGGCGGAAGGGGgcggggcggg                                                                                                                        <  2:370320/135‑1 (MQ=255)
            gctgggCTCGCGGGCAGCCGGTTGCAACTGGATTTCCAGCAGGCGCTGAAAGTGATCCAGGCGGGGGGCGTGGTTCAGCAGCAGGCGCAGGGGGCGCACCTGGCGCAGCTGGGCGTAAGGTGGCTGGGCggggg                                                                                                                      <  6:89734/129‑1 (MQ=255)
                 gcgcgcgGGCAGCGGGTTGCAACTGGCTTTCCAGCAGGCGCTGAATGTGCGCCCGGCGCTCGGCCTGGTGCAGCAGCCGGCGCCGCGGGCGCACCGGGGGCAGCTGGGCGGAAGGGGGCTGGGCGGGGGAGGGt                                                                                                                 <  4:305195/131‑1 (MQ=255)
                 gCTCGGGGGCAGCCGGTTGAAACTGGCTTGCAAGCAGGCGCTGCAAGGGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCACACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGgggtgggt                                                                                                                 <  8:85550/134‑1 (MQ=255)
                  cTCGCGGGCAGCGGGTTGCAAGTGGATTTCCAGCTGGGGGTGAATGTGCGCCGGGCGCTCGGCGTGGTTCAGCAGCAGGCGAAGGGGGCGAACCTGGCGCAGCTGGGGGTAAGGTGGGTGGGGGGGGGCGGGttt                                                                                                               <  8:316985/135‑1 (MQ=255)
                   tCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGttt                                                                                                               >  7:216771/1‑134 (MQ=255)
                   tCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGttt                                                                                                               >  7:74050/1‑134 (MQ=255)
                   tCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGttt                                                                                                               >  2:332783/1‑134 (MQ=255)
                     gcgGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCCGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTa                                                                                                            >  5:113932/1‑135 (MQ=255)
                        ggCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAgg                                                                                                          >  7:144640/1‑134 (MQ=255)
                                         ggCTTTCCAGCTGGCGCTGCATGGGCGCCCGGCGCGCGGCCTGGTTCCGCAGCCGGCGCTGGGGGCGCACCTGGCGCAGCCGGGGGTAAGGTGGCGGGGGGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGTGa                                                                                        <  3:312162/135‑1 (MQ=255)
                                           cTTTCCCGCCGGCGCTGCATGGCCTCCAGGGGCGCGGCCTGGTGCAGCGGCCGGCGCCGGGGGCGCCCCTGGCGCCGCCGGGGGGATGGGGGCGGGGCGGGGGAGGGTTTGTAGGCCAGGGGGGTACGCGTGa                                                                                        <  5:66088/133‑1 (MQ=255)
                                             ttCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTg                                                                                    >  3:329583/1‑135 (MQ=255)
                                                 cccgggCGGTGAAAGTGCTCCTGGGGGTCGGTCTGGTTCCGCTGCGGGGTCTGGGGGCGCACCTGGCGCTCAAGGGCGGATGGTGGGGGGGGGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGt                                                                                <  6:366556/131‑1 (MQ=255)
                                                   ctggCGCTGCAAGGGCGCCCGGGGGGGGGCCGGGTTCAGCCGCCGGCGCCGGGGGCGCACCTGGCGCAGCAGGGCGGAAGGGGGCGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg                                                                              <  2:323414/133‑1 (MQ=255)
                                                   ccggggcTGCATGTGCTCCCGGCGCTGGGCGTGGTTCAGCAGCAGGCGCAGGGGGCGCACCTGGCGAAGCAGGGCGTAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg                                                                              <  3:215573/130‑1 (MQ=255)
                                                   ccggCGGTGCAAGGGCGCCCGGCGCTCGGCCGGGTTCACCCGCCGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGGGGCGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg                                                                              <  4:140809/133‑1 (MQ=255)
                                                   ccggCGCTGCAAGTGCTCCAGGCGCGCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGGGGCGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg                                                                              <  3:146398/133‑1 (MQ=255)
                                                   cagGCGGGGAAAGGGCGCCCGGCGCGCGGCCTGGTTCAGCAGCCGGCGCAGCGGGCGCACCGGGCGCAGCTGGGGGCATGGGGGCGGGGGGGGGGTGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg                                                                              <  2:81868/135‑1 (MQ=255)
                                                   cagGCGCTGCAAGTGCGCCCGGCGCTCGGCCGGGTTCAGCAGCCGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGTAAGGGGGCGGGGGGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg                                                                              <  3:83730/135‑1 (MQ=255)
                                                   cagGCGCGGCAAGTCGGCCTGGGGGGCGGCCGGGGTCAGCAGCAGGCGCTGCGGGCGCACCGGGCGCAGCTGGGCGTAAGGTGGGGGGGCGGGGGAGGGGTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg                                                                              <  2:181520/135‑1 (MQ=255)
                                                                       ggCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGATGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGt                                                          >  1:366881/1‑135 (MQ=255)
                                                                       ggCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGt                                                          >  7:86160/1‑135 (MQ=255)
                                                                             cGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCCCCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGACATGGGGGTACGCGTGAAGTGACGTGGGCAGAGTCTTACATCTCCGTGTCCtg                                                    >  6:201695/1‑135 (MQ=255)
                                                                                 gtGGGTCCGCAGCAGGCGCAGGGGGCGCACGTGGCGCAGCAGGGCGGAAGGGGGCGGGGCGGGGGAGGGGTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGcc                                                <  5:81510/134‑1 (MQ=255)
                                                                                         gccgccggCGCTGCGGGGGCACCGGGCGCCGCCGGGCGTAAGGGGGCTGGGCGGGGGCGGGGTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCctct                                         <  3:295115/128‑1 (MQ=255)
                                                                                                  gcAGCGGGCGCACCTGGCGCAGCTGGGCGGATGGGGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGa                               <  5:192809/135‑1 (MQ=255)
                                                                                                      cGGGCGCACGTGGCGCTCCTGGGCGGATGGTGGCGGGGGGGGGGGGGGTTTGTCTGCCAGGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAAccc                           <  6:39981/135‑1 (MQ=255)
                                                                                                            cagcgggCGCTGCTGGGCGGAAGGGGGGGGGGGGGGGGTGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCt                     <  4:278276/130‑1 (MQ=38)
                                                                                                               cgggCGCCGCTGGGCGGTGGGGGGGGGGGCGGGGGCGGGTTTGCCGGCCAGGGGGGCACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGcc                  <  4:329583/133‑1 (MQ=25)
                                                                                                               cTGGCGCGGCCGGGGGGACGGGGGCGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGcc                  <  8:8846/135‑1 (MQ=38)
                                                                                                                    gcggggggggggAAGGTGGGTGGGCGGGCGCGGGTGGGTCGGCCAGGGGGGTCCGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGt             <  8:216771/129‑1 (MQ=37)
                                                                                                                    gcagctGGGCGTAAGGGGGCGGGGGGGGGGTGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGt             <  8:308090/135‑1 (MQ=38)
                                                                                                                       gccggggggAAGGGGGCGGGGGGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGGGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTAt          <  2:68969/132‑1 (MQ=38)
                                                                                                                         agGGCGGAAGGGGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCg        <  8:239693/135‑1 (MQ=255)
                                                                                                                               gAAGGTGGGTGGGCGGGGGAGGGGTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCt  <  8:42638/135‑1 (MQ=255)
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GGACGCCACATGGCAGTGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCT  >  ACB122/1524596‑1524857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑