| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,686,856 | 0 | T | G | 53.3% | 4.8 / 11.5 | 15 | L90R (CTG→CGG) | CJ019_01584 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (6/1); new base G (1/7); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.01e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCTGGGTCGCGGGCATTCTCGCCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATGGGGTGGACACAGGCGATGCGCCTTATCTGGGCCCGG > ACB122/1686745‑1686984 | ccgggggCGCGGGCATTCCCGCCCATGGGGGCCAGCTGGTGCCGGCCATCGACCTTCCCGCCTTGAGCTGTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCGGGGGCGGGTGCACTACCGAGGCGGCCTGc < 8:115332/132‑1 (MQ=255) tgGGTCGCGGGCATTCTCGCCCATCGTGGCCAGCTGGTTCCGGTCATCGACTTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAg > 4:104486/1‑135 (MQ=255) tgGGTCGCGGGCATTCTCGCCCATCGTGGCCAGCTGGTTCCGGTAATCGCCCTTGCCGCCGTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCCCCCGCCTGGTGCGGGTGCACGACCCAGGCGGCCTGCAg > 4:376406/1‑135 (MQ=255) tgGGTCGCGGGCATTCTCGCCCATCGTGGCCAGCGGGTTCCGGGCATCGCCCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCCCCCGCCTGGTGCTGGTGCACTACCGCGGCGGCcagcag > 7:138793/1‑135 (MQ=255) gcgcgGGACTGCTCGCCCTTGGGGGCCCGGGGGTGCCGGTCATCGACCTGGCCGCCTTGTGGTTTGGCCCGCGGGGGGTCAAACGCACCAGCCCCCGCGTGGGGGGGGGGCACTACCGAGGCGGCCTGCAGCTTg < 6:204362/134‑1 (MQ=255) gCATTCTCGCCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTTGGCAGCCGGCGGTGCCACGCACCAGCCCCCGCCAGGTGCTGGTGCACAACCGCGGCGGCCTGCAGCTTGGGCTg > 7:186236/1‑134 (MQ=255) ggggCCCGCGGGTCCCGGCCAGCGACCTGCCCGCATTGCGCTTGGCCCCGCCGGCGGTCCAACCCACCAGCACCCGCCGGGGGCGGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCa < 4:44988/133‑1 (MQ=255) ggCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCTGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGCGGCGGCCAGCAGCTTGGCCTGATCCTGGAGCAGGCCAcc > 5:136274/1‑135 (MQ=255) gTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCACCGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGACACCCTg > 8:298493/1‑135 (MQ=255) gccgccTGTTGCTGTGGCCAGCCGGCGGGGAAACGCACCTCCACCCGGGTGGGGTGGGCGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAg < 5:175681/135‑1 (MQ=255) gccgccGTGCGCTTGGCCCCCCCGGCGTTCCACCCCACCCCCCCCCGGCTGGTGGGGGGGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAg < 6:314156/135‑1 (MQ=255) cgccTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGCTCCTGGAGCAGGCCACCGAAACCCTGCGGTGCCAACCGGACGAGtt > 1:270290/1‑135 (MQ=255) cgccTGGAGCTTTGGCCCGCCGGCGGGGCAACGCACCTGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGtt < 4:303518/135‑1 (MQ=255) gctggggCCCGCGGGCGGGCCACCCCACCAGCCCCGGCGGGGGGCGGGGCCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCt < 2:270290/130‑1 (MQ=255) ggggCCCGCGGGCGGGGAACCCCCCCGGCACCCGCGGGGTGCGGGGCCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATg < 4:7032/133‑1 (MQ=255) tggtgctggtgcACTACCGAGGCGGCCTGCAGCTTGGCCTGCGCCTGGAGCAGGCCACCGAAACCCAGCGTTGCCAACCGGACGAGTTCCAGCCCTATGGGGTGGACACAGGCGATGCGCCTGATCTGCGCCCgg > 7:202088/1‑135 (MQ=255) tggtgctggtgcACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATGGGGTGGACACAGGCGATGCGCCTTATCTGGGCCCgg > 6:184067/1‑135 (MQ=255) | CCTGGGTCGCGGGCATTCTCGCCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATGGGGTGGACACAGGCGATGCGCCTTATCTGGGCCCGG > ACB122/1686745‑1686984 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |