| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 2,950,166 | 0 | C | G | 56.2% | 4.7 / 16.6 | 16 | intergenic (+64/‑132) | spuC_2/puuC_1 | Putrescine‑‑pyruvate aminotransferase/NADP/NAD‑dependent aldehyde dehydrogenase PuuC |
| Reads supporting (aligned to +/- strand): ref base C (2/5); new base G (0/9); total (2/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.80e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TAGCCGTAGACCGCACGGCGCGGCTGGTCGGCAAGCTGTAAGCCGCTGACAAGGGCAACGCGCTTCCCTGTGGGAGCGGGCACGCCCGCGAACACCGGCGAAGCCGGTGCCATCCACCGTGTCGCCTGATTCGCGGGCA‑TGCCCGCTCCCACAGAGGGAGTGCGGCGTGCTTGCGGACTGTGTGGTACCTGTTACTTGCGCAAGAAGGACCGCCACGACGGTCGTTTGGA > ACB122/2950062‑2950291 | tcgCCGTAGCCCGCACGGCGGGGCTGGGCGGCAAGCTGTACGCCGCTGACAAGGGCAACGCGCTTCCCTGGGGGAGCGGGCACGCCCGCGAACACCGGCGAAGCCGGTGCCATCCACCGTGTCGCCTGATTcgcg < 6:94654/133‑1 (MQ=37)tAGCCGTAGACCGCACGGCGCGGCTGGTCGGCAAGCTGTAAGCCGCTGACAAGGGCAACGCGCTTCCCTGTGGGAGCGGGCACGCCCGCGAACACCGGCGAAGCCGGTGCCATCCACCGTGTCGCCTGATTcgcg < 3:660/135‑1 (MQ=255) gCCGTAGACCGCAGGGGGGGGGGGGTCGGAAAGCTTTAAGCGGGTGACTTGGGAAACGCGCTTCCCTGTGGGTGGGGGCACGCCGGCGAACACGGGCGAAGCGGGTGCAATCCACCGTGTGGCCTGATTCGCgg < 4:43438/134‑1 (MQ=255) ggCACGCTGAACGCCGCGCACAAGGCCACCGCGCTGCCCTGGGGGAGGGGGCACCCCCGCCAACACCGGCGACGCGGGTGCCACCCCCCGTGCCGCCTGATGCGCGGGCA‑GGCCCGCCCCCACAGAGGGAGTgcg < 4:112818/135‑1 (MQ=255) gccccgCTGTCCGCCGCGCACACGGGCACCGCGCTCCCCGGGGGGCGCGGGCACCCCCGCGAACCCCGGCCACGCGGGTGCCACCCCCCGGGGCGCCGGATTCGCGGGCA‑TGCCCGCCCCCACAGAGGGAGTgcg < 2:44997/130‑1 (MQ=12) acgCCGCTGACTCGGGACACGGGCTGCGGTGTGGGTGCGGGCAGGCCCGCGTACACCGGCCACGCGGGTGCCTTCCACGTTGGCGCCT‑TTTTGTGTGTATTGCCCGTTCCCACAGAGGGAGTGCGGCGTGCtttg < 8:176602/133‑3 (MQ=255) aGCCGCTGACAAGGGCACCGCGCTTCCCTGTGGGTGCGGGCACGCCCGCGAACACCGGCTAAGCGGGTGCCATCCACGGTGTCGCCTGATTCGCGGGCA‑TGCCCGCCCCCACAGAGGGAGTGCGGCGTGCTTGc < 8:113664/134‑1 (MQ=37) cAAGGGCAACGCGGTGCCGTGGGGGAGGGGGCACGCCCGGTAACACCGGCGAAGCGGGTGCCATCCACCGGGTCGCCTGATTCGCGGGCA‑TGCCCGCTCCCACAGAGGGAGTGCGGCGTGCTTGCGGACtgtg < 6:329607/133‑1 (MQ=25) aCGCGCGCCCGTGGGGGCGCGGGCAGGCCCGCTAACACGGGCCACGCGGGTGCCATCCACCGGGCCGCCTGATGCGCGGGCA‑GGCCCGCTCCCACAGAGGGAGTGCGGCGTGCTTGCGGACTGTGTGGTACCTGt < 4:359804/135‑1 (MQ=255) cTTCCCGGGGGGCGGGGGCACGCCCGCGAACACCGGCGACGCCGGTGCCATCCACCGTGTCGCCTGATTCGCGGGCA‑TGCCCGCTCCCACAGAGGGAGTGCGGCGTGCTTGCGGACTGTGTGGTACCTGTTACtt < 1:27701/135‑1 (MQ=37) cTGTGGGAGCGGGCACGCCCGCGAACACCGGCGAAGCCGGTGCCATCCACCGTGTCGcc < 7:247391/59‑1 (MQ=255) cTGTGGGAGCGGGCACGCCCGCGAACACCGGCGAAGCCGGTGCCATCCACCGTGTCGcc > 8:247391/1‑59 (MQ=255) cGGCTAAGCGGGTGCCATCCACCGGGCCGCCTGATGCGCGGGCT‑GCCCCGCCCCCACAGAGGGAGTGCGGCGTGCTTGCGGACTGTGTGGTACCTGTTACTTGCGCAAGAAGGACCGCCACGACGGTCGTTTGGa < 3:182478/135‑1 (MQ=255) cGGCGACGCGGGTGCCACCCACGGTGTGGCCTTATTGGCGGCCT‑TGCCCGCGCCCACAGAGGGAGTGCGGCGTGCTTGCGGACTGTGTGGTACCTGTTACTTGCGCAAGAAGGACCGCCACGACGGTCGTTTGGa < 3:167601/135‑1 (MQ=255) cGAAGCCGGTGCCATCCACCGTGTCGCCTGATTCGCGGGCA‑TGCCCGCTCCCACAGAGGGAGTGCGGCGTGCTTGCGGACTGTGTGGTACCTGTTACTTGCGCAAGAAGGACCGCCACGACGGTCGt < 7:85270/127‑1 (MQ=255) cGAAGCCGGTGCCATCCACCGTGTCGCCTGATTCGCGGGCA‑TGCCCGCTCCCACAGAGGGAGTGCGGCGTGCTTGCGGACTGTGTGGTACCTGTTACTTGCGCAAGAAGGACCGCCACGACGGTCGt > 8:85270/1‑127 (MQ=255) | TAGCCGTAGACCGCACGGCGCGGCTGGTCGGCAAGCTGTAAGCCGCTGACAAGGGCAACGCGCTTCCCTGTGGGAGCGGGCACGCCCGCGAACACCGGCGAAGCCGGTGCCATCCACCGTGTCGCCTGATTCGCGGGCA‑TGCCCGCTCCCACAGAGGGAGTGCGGCGTGCTTGCGGACTGTGTGGTACCTGTTACTTGCGCAAGAAGGACCGCCACGACGGTCGTTTGGA > ACB122/2950062‑2950291 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |