Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,134,5670TG52.2% 0.2 / 11.9 23L143V (TTG→GTG) CJ019_02952hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (11/0);  new base G (12/0);  total (23/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGA  >  ACB122/3134444‑3134685
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aGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCCCGCGGTGGCCGAACAg                                                                                                             >  7:288772/1‑135 (MQ=255)
  cGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGGTGGCCGAACAGCt                                                                                                           >  4:258616/1‑135 (MQ=255)
   gTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGGTGGCCGAACAGCTg                                                                                                          >  4:281582/1‑135 (MQ=255)
    tGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACAACCTCGGACAACGTCGCGGCCGGCCGCGGGTTGGCCGCACAGCggg                                                                                                         >  8:128239/1‑135 (MQ=255)
      aTCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCCGCCCGCCGCCCGGTGGCCGCACCGCGGGTg                                                                                                       >  7:187265/1‑135 (MQ=255)
                  cTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCCGCCGGCCGGGCGGTGGCCGCACAGCTGGGGGGCGCCCCcgcc                                                                                           >  5:201438/1‑133 (MQ=255)
                         gcATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGGCCGCCGCGCGGTGGGCGCCCAGCAGGGGCGCCCCCCCcgcggccacg                                                                                    >  1:270185/1‑131 (MQ=255)
                               aGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGGCGGCCGCGGGTGGGCCGCCCCGCTGGTGCGCGCCACCGGCGCCCAGCGcccgg                                                                              >  3:109584/1‑132 (MQ=255)
                               aGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGGTGGCCGAACACCTGGTGCGCGACACCCGCCGCAAGGGCCGcc                                                                              >  6:5781/1‑134 (MQ=255)
                                    gcagGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGGTGGGCGGACCGCTGGGGGGCGCCCCCCGCGGCCAGGGgcgccaccgg                                                                         >  3:118207/1‑129 (MQ=255)
                                    gcagGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACCTCCCCGCCCGCCGCGCCTTGGGCGCACAGCTGGTGCGCGACCCCCGCGGCAACGGCCGCaaacag                                                                         >  6:88618/1‑130 (MQ=255)
                                                                        cAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGGGCGCGCCACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCtgtg                                       >  4:303707/1‑133 (MQ=255)
                                                                                   caacaaCACCACCTCGGACAACGTCGCGGCCGGCCGCGGGTTGGCCGCACAGCTGGTGCGCGTCAACGGCGGCAAGGGCCGCATCCTGGTGTTGAACGGCTTCTACGGGGGGGCGGTCTGTGGGATGCGcgacga                          >  6:192064/1‑135 (MQ=255)
                                                                                   caacaaCACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGCCACCGGCGGCCAGGGCCGCATCCTGGTGTTAAACGGCGTCTACGGGGTGCCGGTCTGTGCGCTCCGcgacga                          >  4:25857/1‑135 (MQ=255)
                                                                                   caacaaCACCACCTCGGACAACGTCGCGGCCCGCCCCGCGGTGGGCGCACCGCTTGTGCGCGCCACCCGCCGCCAGGGCCGCCTACTGGGGTTTAAAGGCGTCGACGGGGGGCCGGTCTGTGGGGTCCGcgaaga                          >  2:11143/1‑130 (MQ=255)
                                                                                   caacaaCACCACCTCGGACAACGTCGCGGCCCGCCCCGCGGTGGGCCGACAGCGGGGGGGCGCCACCCGCGGCCAGGGCCGCCTCCGGGGGGTGAACGGCGTCGACCGGGTGGCGGGCGGTGGGCTCCGCGacgc                          >  2:4167/1‑134 (MQ=255)
                                                                                     acaacaCCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGCTCCGCGacgc                          >  7:70560/1‑132 (MQ=255)
                                                                                        acacCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACCCCGGCGGCAAGGGCCGCATCCTGGTGTTCAACCGCGTCTACGGGGTGCCGGTCTGTGCGCTCCGCTACGACGAGt                     >  6:97043/1‑135 (MQ=255)
                                                                                            caccTCGGACAACGTCGCGGCCGGCCGCGCGGTGGCCGAACAGCTGGTGCGCGACCCCGGCGGCAAGGGCCGCCTCCGGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGtt                    >  6:81670/1‑132 (MQ=255)
                                                                                                 cGGACAACGTCGCGGCCGGCCGCCCGGTGGCCCAACAGCTGGTGCGCGCCACCGGCGGCCAGGGGCGCAGCCGGGGGTTGAACCGCGTGTACGGGGGGCCGGGCTGTGCGCTGCGCTGCGCCGCGGTgaagaacg            >  6:301919/1‑133 (MQ=255)
                                                                                                    aCAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGGGCGCGCCACCCGCGGCCAGGGGCGCCTCCAGGTGTTGAACCGCGTGTAAGGGGTGCCGGTGTGTGGGGTACGCGACCCCCAGTTGAATAAAGTAc         >  4:201519/1‑135 (MQ=255)
                                                                                                           cgcgGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGCCACCGGCGGCAAGGGCCGCCTCCTGGTGTTCAACGGCGTCTACGGGGGGCCGGTCTGTGCGCTCCGCGACGGCGAGTTGAAGAAGGTACAGGCCgc  >  5:104625/1‑134 (MQ=255)
                                                                                                           cgcgGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCGTCGACGGGGTGCCGGTCTGTGCGGTCCGCTACGCCGAGTTGAAGAAGGTACTGGGCGa  >  7:382699/1‑135 (MQ=255)
                                                                                                                           |                                                                                                                      
AGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGA  >  ACB122/3134444‑3134685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑