Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,586,4040AG37.0% 26.9 / 10.3 32D128G (GAT→GGT) CJ019_03399hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (8/9);  new base G (10/0);  total (21/11)
Fisher's exact test for biased strand distribution p-value = 8.83e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGTTGGTGGTGGCCGAACAGTTTGGCACCCTCAATACATTGCACCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGATCCTCGGTTCCAGCCTGTTCG  >  ACB122/3586272‑3586523
                                                                                                                                    |                                                                                                                       
cGTTGGTGGTGGCCGAACAGTTTGGCACCCTCAATACATTGCACCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCCCGTCCGGGCCGACCGGTCGGGCATTGCGCGGCGCCGCGCCCGCGCGGGCtt                                                                                                                       >  8:112493/1‑135 (MQ=255)
cGTTGGTGGTGGCCGAACAGTTTGGCACCCTCAATACATTGCACCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCCGAGCGGGGtt                                                                                                                       >  2:203418/1‑135 (MQ=255)
  ttGGTGGTGGCCGAACAGTTTGGCACCCTCAATACATTCCACCCAGGTCGTTTCGACCGGGGCCTGGGCGGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTc                                                                                                                     <  2:228021/135‑1 (MQ=255)
         gggCCGACCTGTTCGGCACCCGCAATACATGGCCCCCAGGCCCAAGCGCCCTGGGCCTGGGCCGTCCGCCAGGCAGGGCCGGGCCGCCCGGGCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTCGGCCAgg                                                                                                              <  8:240981/134‑1 (MQ=255)
                  aGTTTGGCACCCTCAATACATTGCACCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGGGCGGCGCCCCCCCCCCGCGGGGTTTCGGCCCCGCCAACGCCg                                                                                                     >  5:90746/1‑135 (MQ=255)
                                 aTACATTGCACCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGTCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGGCACAGCGGGATTTAGGCCATGACATATCCGAGCTTAGTGGTTaac                                                                                      >  2:257730/1‑133 (MQ=255)
                                 aTACATTGCACCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCCCGCCCGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGGGAGTa                                                                                        >  2:110149/1‑133 (MQ=255)
                                          aCCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCCGCGCGGGGTTTAGGCCCGGGCCTAGCCGCGCGGCGGGGTTACCTGGGCggcc                                                                             >  3:47311/1‑132 (MQ=255)
                                          aCCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCCCGGCCGAGCGGGTTTTAGGGCAAGACAAAGCCGCGCAGCGTGATTTACTgggccacc                                                                             >  4:308677/1‑129 (MQ=255)
                                                                 ctgggccGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGGGCGGCCCCCCGCCCGGGCGGGGTTTAGGCCCGGCCCTATCCGAGCGGCGTGTTTAACATGGCGCCCACCGCAAACCCCCGGTGGgcggg                                                      >  2:218922/1‑134 (MQ=255)
                                                                   gggccGTGCGCCAGGCACGTCCGGGCCGCCCGTTCGGGCATTGGGCGGCGCCGCGGCCCGGCGGGCATTAGGCCCGGGCATCCGCCCGCGGCGGGATTTCCTGGGCGCCCACCGCAAACGCCcggggcggggggg                                                    >  8:27875/1‑132 (MQ=255)
                                                                      ccGTGCGCCAGGCACGTCCGGGCCCACCGTTCGGGCATTGCGCGGCGCCGCGGCACGGCCGGAATTAAGCCACGACAACGCCGAGCTGCGTGATTAACAGGGCTACCAAAGCAAACCCCCGGTGCgccgcggaac                                                 >  8:391089/1‑131 (MQ=255)
                                                                       cGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCCGCGCGGGGTTTGGGGCCGGGCCTCGCCGCCCGGGGTGGTTTACAGGGCGCCCACGGCAAACCCCCGGGGCgcggcgggccg                                                >  8:16273/1‑130 (MQ=255)
                                                                       cGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCCCGCCCGCGCGGGGTTTAGGGCCCGACATCGCCGCGCGGCGGGGGTTCCAGGGCGCCCACCGCCAACCCCGGgggcggggcggtccc                                                >  1:39755/1‑130 (MQ=255)
                                                                           cgcCAGGCACGTCCGGGCCGACCGTTCGGGCATTGGGCGGCGCCGCGCCAGAGCGGGATTTAGGCCAGGACATCGCCGAGCTGCGGGATTACCTGGCCGCCAACCGCAAACGCCGGGTGCGCGGCGTACCggggg                                            >  4:179500/1‑133 (MQ=255)
                                                                            gcCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGCGCGGGGTTTAGGCCAGGCCCTCGCCGCGCTGCGTGATTACCTTGCCGCCAACCGCAAACGCCGGGTGCGCGGCGTACCGGGTgc                                           >  2:272277/1‑135 (MQ=255)
                                                                            gcCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGGTTTAGGGCCGGCCATCGCCGCGCGGCGTGGTTTCCTGGCCGCCAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTgc                                           >  1:6635/1‑135 (MQ=255)
                                                                            gcCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCACAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTgc                                           >  7:321708/1‑135 (MQ=255)
                                                                            gcCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCCCCCGCGCGGGATTTCGGCCAGGACCTCGCCGCGCTGCGGGATTACCTGGCCGACAACGGCCAACGCCGGGTGGGCGGCGTTCCGGGGgc                                           >  8:132548/1‑135 (MQ=255)
                                                                            gcCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCCCGCCAGCGCGGGGTTTCGGCCAGGACCTCGCCGCGCGGCGGGGTTACCTGGGCGGCCACCGCCAACGCCCGGTGGGCGGGGTTCCGGGGgc                                           >  4:279314/1‑135 (MQ=255)
                                                                                    gggccGGCCCGCCGGTGCGGGCTTGGCGGGGCGCCGCGCCAGAGCGGGATTTCGGCCAGGACAGCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGAtg                                   <  8:185714/132‑1 (MQ=255)
                                                                                                 ggTTCGGGATTGGCGGGGCGCGGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGAt                      <  6:90746/134‑1 (MQ=255)
                                                                                                  gTTCGGGCATTGCGCGGCGCCCCGGCCGGGCGGGGTTTCGGGCACGCCATACCCCAGCAGGGTGTTTACCTTGCCGACAACGGCAAACGCCCGGTGCGCGGCCTTCCGGGTGCGCCCGCTGTGGCGGTGTGGAt                      >  6:74455/1‑134 (MQ=255)
                                                                                                      gggCTTGGCGCGGCGCCGCGCCAGAGCGGGATGTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGGGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGATCCTCg                 <  4:198226/135‑1 (MQ=255)
                                                                                                             gcgcGGCCCCGCGCCAGAGCGGGATTGCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGATCCTCGGTTcc            <  3:279314/133‑1 (MQ=255)
                                                                                                               gcggcgCCGCGCCTGCGCGGGCTTGCGGCCAGGACAGCGCCGAGCGGCGTGAGCACCGGGCCGACAACGGCAAACGCCGGGGGTGCGGCGTACCGGGGGCGCACGATGTGCCGGTGTGGATCCTCGGTTCCAGcc        <  4:294879/135‑1 (MQ=255)
                                                                                                               gcggcgCCGCGCCAGAGCGGGATTTAGGCCAGGACATAGCCGAGCTGCGTGTTTTACTGGGCGACAACGGCAAACGCCGGGTGCGCGGCGTACCCGGTGCGCCCGATGTGCCGGGGTGGATCCACGGTTACCGcc        >  6:384665/1‑135 (MQ=255)
                                                                                                                gggcgCCGCGCAAGTGCGGTTTTTCGGCCTGTACATCGCCGAGCGGCGTGATTAGCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGATCCTCGGGTCCAGCCt       <  1:249784/134‑1 (MQ=255)
                                                                                                                cggcgCCGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGATCCTCGGTTCCAGCCt       <  7:37575/135‑1 (MQ=255)
                                                                                                                cggcgCCGCGCCAGAGCGGGATTGCGGCCAGGACATCGCCGCGCTGCGTGATTCCCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGATCCTCGGTTCCAGCCt       <  1:82182/135‑1 (MQ=255)
                                                                                                                cggccCCGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGATCCTCGGTTCCAGCCt       <  4:195711/135‑1 (MQ=255)
                                                                                                                      cgccccACAGCGGGAATTAGGCCAGGACATAGCCCACCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTAACCGGTTCGCACCAATTGCCGGTGTGGAAACAAGGTTACAGccctgtct  >  6:114558/1‑128 (MQ=255)
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CGTTGGTGGTGGCCGAACAGTTTGGCACCCTCAATACATTGCACCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGATCCTCGGTTCCAGCCTGTTCG  >  ACB122/3586272‑3586523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑