Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,135,6070AC50.0% 8.7 / 10.9 39Q32P (CAG→CCG) tgnC(Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (9/10);  new base C (17/2);  total (26/13)
Fisher's exact test for biased strand distribution p-value = 1.28e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGCGT  >  ACB122/4135482‑4135734
                                                                                                                             |                                                                                                                               
cccTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGgc                                                                                                                        >  6:88676/1‑135 (MQ=255)
 ccTCACCGAATCCGCCAGGGGGATTGTTGAATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCAATTGGCGCGAGGCCAAGAGCGGCAAGACATTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGgcg                                                                                                                       <  6:45688/135‑1 (MQ=255)
 ccTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCGGGgcg                                                                                                                       >  1:140853/1‑135 (MQ=255)
 ccTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGgcg                                                                                                                       <  3:87140/135‑1 (MQ=255)
  cTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCCCCGCCCCCGCCGGGgcgc                                                                                                                      >  5:163358/1‑135 (MQ=255)
      ccGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCAGGCCTGGgcgccgcg                                                                                                                  >  1:359242/1‑131 (MQ=255)
      ccGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCACCt                                                                                                                  >  2:285206/1‑135 (MQ=255)
      ccGAATCCGCCAAGGGGATTGTTGCAGGACCCTCGTTGGTTTCCAGTTGTGCAGCGCGGGCCAAGGGGGCGAGGCCCAGCGCGGCAAGACCTTCGACAGCCACAACCCGCCCCCCCCCCAGGCCTGGGCGCAACt                                                                                                                  <  6:163358/135‑1 (MQ=255)
       cGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCAGGgcgccccag                                                                                                                 >  4:170358/1‑130 (MQ=255)
              gCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCCACTGCCCGACg                                                                                                          >  4:52331/1‑135 (MQ=255)
              gCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCACCAGCCCGACg                                                                                                          >  1:94701/1‑135 (MQ=255)
              gCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGACCAGAGCGGCAAGACCTTCGCCAGCCTCAACCCGGCCACCGCCCCGGCCGGGGCGCAACGGCccgccg                                                                                                          >  7:280777/1‑135 (MQ=255)
                          gttgCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCCGGCCTGGGCGCCACGGCCCGCCGCCGATGCAGCCg                                                                                              >  1:125626/1‑135 (MQ=255)
                                        gttcgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCTGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCAGGGCGCCAATGCCCCCCGCCGATGCAGCCGCTGGGGCGCTGGcc                                                                                >  2:379159/1‑135 (MQ=255)
                                        gttcgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCGGGGCGCCACTGCCCCCCGCCCAGTCAGCCGATGTGGAGGTgggc                                                                                >  2:154931/1‑133 (MQ=255)
                                          tcgttTCCAGATGTGCATCGACGGCCAATGGAGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACAGCCCGCCGCCTATTACGACGATGTTGAGCTTGCCGt                                                                              >  7:188894/1‑135 (MQ=255)
                                                ccAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCAGCGGGCCCCTGCCCCCCCCCCACCAACCCGCAGGGGAGCGGGGCGTTCAgtg                                                                        >  7:73198/1‑133 (MQ=255)
                                                          aTCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGCCGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGt                                                              >  6:274514/1‑135 (MQ=255)
                                                            cGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCAACTGCCCGCCCCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGc                                                            >  3:62189/1‑135 (MQ=255)
                                                              aCGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCGGGGCGCCACTGCCCCCCGCCGCTGACGCCGCTGTGGCGCTGGCCGGTCCGGCCGCCCCGCGGGccg                                                          >  8:268140/1‑134 (MQ=255)
                                                                   cAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCCACTGCCCGACGCCGCTGAAGCCGATGTGGAGCTGGGCGTTCAGGCCGCCCCGCGTGCCGTCgcc                                                     >  2:121690/1‑133 (MQ=255)
                                                                         cgcgAGGCCCAGAGCGGAAAGCCCTTCGACAGCCGCACCCCGGACACCGCCCAGGCCTGGGCGCAACTGCCCTACGACGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAg                                               <  4:230658/135‑1 (MQ=255)
                                                                              ggCCCAGAGCGGCAAGCCCTGCGACAGCCTCACCCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTg                                          <  8:106818/134‑1 (MQ=255)
                                                                              ggCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTg                                          <  4:123212/134‑1 (MQ=255)
                                                                                      gCGGCAAGACATTCGAAAGCCTCAACCGCGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCgcagca                                  <  8:316951/135‑1 (MQ=255)
                                                                                            aGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAAATGCCCGACGCCGATGAATCCGATGTGGCGCTGGCCGTTAAGGCCGCCCAGCGTGCCGTCGACAGCAAGGCGTGGCGCAGCATCACCg                            >  2:255786/1‑135 (MQ=255)
                                                                                                 ttCGACCGCCTCACCCCGCCCCCCCCCCAGGCGGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAcc                       <  3:52331/135‑1 (MQ=255)
                                                                                                    gACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAccgc                     <  6:64432/134‑1 (MQ=255)
                                                                                                     aCAGCCTCAACCCGGCCCCGGCCCTGGCGGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCTTGTCGCAGCATCACCGCTAccgc                     <  5:176054/133‑1 (MQ=255)
                                                                                                        gCCTCACCCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCGGGCCGTGCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGTCGCAGCATCACCGCTACCGCCcgcg                <  8:183420/135‑1 (MQ=255)
                                                                                                        cccTCACCCCGGCCCCCGCCCCGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg                <  5:214162/134‑1 (MQ=255)
                                                                                                            cAACCCGGCCACCGCCCCGGCCTGGGCGCAACAGCCCGACCCCGCTGAAGCCGCTGTGGAGCAGGCCGTTTAGGCCGCCCCCCGTGCCGTCGCCAGCAACGCCTGGCGCCGCCAAACCGCTCACGCCcgccgcca            >  2:86931/1‑133 (MQ=255)
                                                                                                            cAACCCGGCCACCGCCCCCGCCAGGGCGCACCTGCCCGCCGCCGCTGAAGCCGATGTGGAGCTGGCCGTTAAGGCCGCCCCGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCGACCGCCCCCGGcca            >  8:133209/1‑133 (MQ=255)
                                                                                                            cAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCGTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCCCGGcca            >  8:380878/1‑133 (MQ=255)
                                                                                                                ccGGCCACCGCCCCGGCCTGGGCGCAACTGCCCGCCGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCCGCGTGCCTTCGACCGCAAGGCGTGGCGCCGCATCACCGCTACCGCCCGCGGCAAgctg        >  8:106983/1‑135 (MQ=255)
                                                                                                                  ggCCTCCGCCCCGGGGGGGCCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCTCTACCGCCCGCGGCAAgctgct      <  3:86357/135‑1 (MQ=255)
                                                                                                                      aCCGCCCCGGCCTGGGCGCCACTGCCCGCCGCCGCTGAAGCCGCTGTGGTGCTGGCCGTTCAGGCCGCCCAGCGTGCCGTCGCCAGCCAGGCGTGGCGCCGCATAACCGCTACCCCCCCCGGCAAGCGGCTGcgt  >  6:125229/1‑135 (MQ=255)
                                                                                                                      aCCGCCCCGGCCTGGGCGCCACTGCCCGCCGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCGTCGCCCGCAAGGCGTGTCGCCGCCTCACCGCGACCCCCCCCGGCCAGCTGCTGcgt  >  8:59010/1‑135 (MQ=255)
                                                                                                                      aCCGCCCAGGCCTGGGCGCAACTGCCCGCCGCCGCTGAAGCCGATGTGGCGCTGGCCGTTGAGGCCGCCCAGCGAGCCTTCGCCAGCAAGGCGTGGCGCCGCATAACCGCTACCGCCCGCGGCAAGCAGCGGcgt  >  6:295061/1‑135 (MQ=255)
                                                                                                                             |                                                                                                                               
CCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGCGT  >  ACB122/4135482‑4135734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑