Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,245,1450AC51.8% 7.1 / 19.5 29L178R (CTG→CGG) CJ019_03982Crotonyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (4/9);  new base C (7/7);  total (11/18)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.55e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGC  >  ACB122/4245016‑4245268
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ccGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCCGGCAACTGCTCGTCGGCGCAGACTTCGCTGCCCAACCCGGCGGCCCCGgcg                                                                                                                        >  5:355988/1‑135 (MQ=255)
ccGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCCACCCGGCGGCCCGGgcg                                                                                                                        >  6:307511/1‑135 (MQ=255)
 cggcgcgTATCAGCCGCTTGGGCTGCGCATAGGCGAAGGGCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGgcgc                                                                                                                       <  3:12958/132‑1 (MQ=255)
 cGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGGCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGgcgc                                                                                                                       <  1:11617/135‑1 (MQ=255)
    cgcgTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGGCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGTGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAt                                                                                                                    <  4:278710/135‑1 (MQ=255)
    cgcgTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGGCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAt                                                                                                                    <  2:216132/135‑1 (MQ=255)
                      tCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACtcgt                                                                                                  >  8:191897/1‑135 (MQ=255)
                        tGCGCATCGGCGATGGGGGGGCCTTGGCCCTGGCGCCCGGCATGTGCGGCGGCAAGGGCTGGCAAGTGCTGGTCGGCGCAGACTTCGCTGACAACCCCGGCGCCCTGGGCGGGATCGGCGCCCCACAACtcgtcg                                                                                                <  4:279379/135‑1 (MQ=255)
                               aGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                         <  7:191897/135‑1 (MQ=255)
                               aGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCGCGTCGGCGCAGACTGCGCTCACCACCCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAAca                                                                                         <  1:70185/135‑1 (MQ=255)
                                ggCGATGGGCGGGCCATTGGCCTGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGGCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAAca                                                                                         <  6:283066/134‑1 (MQ=255)
                                ggCGACGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTGCGGCGGCACGGCCCGGCAAGTGCTCGGCGGGGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                         <  2:219111/134‑1 (MQ=255)
                                     cgtgggggCAAGTGCCCAGGCCCCGGGCCAGTGCGGCGCCAACGTCTGGAAACTGCTCGTCGGCGCAGACTGCGGTGTCCACCCCGGGGGCCCGGGCGCTTTCGGCGCCCCACAACTCGTCGAGGAACAGCAGgc                                                                                   <  8:347507/130‑1 (MQ=255)
                                            gCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCCGCGCCCCACAACTCGTCGAGGAACCGCAGGCGCGTGGc                                                                            >  4:129006/1‑135 (MQ=255)
                                              cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGCGCGATCGGCGCCCCACAACACGGCGAGGGACAGCAAGCGCGTgggca                                                                          >  7:125098/1‑132 (MQ=255)
                                              cATTGGCCAGGCGCCCGGCCAGTTCGGCGGACACGGCAGGCAACTGCTCGTCGGCGCAGACTGCGCGGCCCCACCCGGAGGCCAGGGCGCGAGAGGCGCCCCCCCACGCGTCGAGGAACAGCCGGCGCGTGGccc                                                                          >  5:247134/1‑134 (MQ=255)
                                                   gCCAGGGGCCCGGCCAGTGCGGCGGCAACGGCAGGCACCTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCg                                                                     <  8:237963/135‑1 (MQ=255)
                                                        gcgcCCGGCCAGTTCGGCGGCACCGGCAGGCACCTGCGCGTCGGCGCAGAATTCGCCGACCCACCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                                <  6:247134/135‑1 (MQ=255)
                                                                ccAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATAGGCGCCCCCCCACTCGTCGAGGGACCGCCGGCCCGTGGCCTGCTCGCGGCCGATCAGcc                                                        >  7:44055/1‑135 (MQ=255)
                                                                ccAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCCCAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGcc                                                        >  7:309118/1‑135 (MQ=255)
                                                                                 gcaggcaACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCCCCACTCGTCGAGGCACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTg                                       >  4:267221/1‑135 (MQ=255)
                                                                                          tGCGCGGCGGCGCAGACTGCGCTCACCACCCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                              <  6:115789/135‑1 (MQ=255)
                                                                                          ggggggtcgGCGCATCATGCGCGGACCACCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                              <  6:341917/130‑1 (MQ=255)
                                                                                               ggcgGCGCAGACTGCGCTGCCCACCCCGGCGGCCCGGGCGCGAGCGGCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCggg                         <  7:34558/133‑1 (MQ=255)
                                                                                                    cgcAGACTGCGCTCACCACCCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt                    <  5:287448/135‑1 (MQ=255)
                                                                                                       aGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCCACTCGTCGCGGAACCGCAGGCGCGTGGCCTGCTCGCTGCCGCTCCGCCGGGGCCGGTGGCCGCTGGCGCCGGCCTCGGGGCGGTAGc                 >  8:34558/1‑135 (MQ=255)
                                                                                                         aCTTCGCTGACCACCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGccc               <  7:321169/135‑1 (MQ=255)
                                                                                                              cgtgcccACCCCGGCGGCCTGGGCGCTTGCGGCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCGCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGc           <  6:2370/129‑1 (MQ=255)
                                                                                                                      aCCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTTGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCCGGGCTGTAGCCCAAGCTGGTGTAGc  >  8:357999/1‑135 (MQ=255)
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CCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGC  >  ACB122/4245016‑4245268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑