Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,277,0430TG37.9% 21.5 / 11.9 29G278G (GGT→GGG) hbd3‑hydroxybutyryl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (4/14);  new base G (11/0);  total (15/14)
Fisher's exact test for biased strand distribution p-value = 5.00e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGGCCTGGACACCCTGCTGGCAATCATGGAGGCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGACCGGGCCATGGCATTGTTTGCCGAAAAGGGCTTTGGCCAGGTCAGCAT  >  ACB122/4276914‑4277171
                                                                                                                                 |                                                                                                                                
cGGCCTGCACCCCCTGCTGCAAACCAGGCCGGCCTTCCCCGAAGGCTCCAACTACCGCAAAACCCGCCCTCCTCCACGGCTCAAGGAAAGGGGCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTttctt                                                                                                                             <  1:1915/135‑1 (MQ=255)
 ggCCTGGACACCCTGCTGGCAATCATGGAGGCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGATCGGTCGCGGgttgtcc                                                                                                                            >  1:103905/1‑131 (MQ=255)
 ggCCTGGACACCCTGCGGGAAATCATGGAGGCCTTCCACGACGGCTTCAACGACAGAAAATCCCGCCCTGCTCCACTGCTCAAGGAAATGGGCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTttctt                                                                                                                             <  4:119027/134‑1 (MQ=255)
               gctgGCAATCATGGAGGCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTgaggag                                                                                                               >  4:61546/1‑134 (MQ=255)
                         aTGGAGGCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTGACCTACTGAGGCGGAAGCGcccag                                                                                                    >  2:342412/1‑132 (MQ=255)
                           ggAGGCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGGTGAACGACAGAGGCGCCAGCGCCCTGcc                                                                                                  >  1:203770/1‑135 (MQ=255)
                                    ccACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCGGGGGCGCAAGAGCGGTCGCGGTTTCTTAACCTACAGAGGAGCAAACCCCAAACCCACgggcc                                                                                          >  8:386759/1‑131 (MQ=255)
                                      aCGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGGTACCCTACTGAGGAGCCAGCCCCAAGCCCCCCGTCAATg                                                                                       >  4:21828/1‑135 (MQ=255)
                                              ttCAACTACAGAAAAAACCGCCCGGCCCCCCGGCTCAAGCAAATGGCCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATg                                                                               <  8:158461/135‑1 (MQ=255)
                                              ttCAACGACAACAAATACCGCCCTGCCCCCCTGCTCAAGTAAAGGGCCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATg                                                                               <  8:366854/135‑1 (MQ=255)
                                                   ccacacCAAATCCCGCCCTGCGCCACTGCCCCAGGAATGGGCCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCtg                                                                          <  2:203770/133‑1 (MQ=255)
                                                         cAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTGACCGACTGAGGCGCAAGCGCCAAGCCCCCCGGCAAAGCCGCCAAGCCCGGTGCCac                                                                    >  8:5204/1‑134 (MQ=255)
                                                         cAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTCGTAACCGACTGTGGACAAAGCCCCCTGCCCCCCGGCAAATCCGCCAAGCGCGGTTccca                                                                    >  8:381629/1‑133 (MQ=255)
                                                          aaaTACCGCCCTGCTCCACTGCTCAAGGAAATGGGCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAAc                                                                   <  6:149216/135‑1 (MQ=255)
                                                                     tGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTGACCGACTGAGGTGCAAGCCCCCTGCCCCCCGGCAATGACGCCAAGCGCTGTGCCCACCTTgaggctcg                                                        >  8:381819/1‑131 (MQ=255)
                                                                     tGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTGTTGAACAAAAGAGGAGCAATCCCCAAGCCCCCGCGCAAAGCCGCCATGCGCTGTGGCAACATTtaggctcg                                                        >  4:296375/1‑131 (MQ=255)
                                                                        tCCACTGCTCAAGG‑ATTGGGCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGt                                                    <  8:66140/135‑1 (MQ=255)
                                                                         ccACTGCTCAAGGAAATGGGCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGt                                                    <  7:326366/135‑1 (MQ=255)
                                                                          cACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTCTTGACCGACTGAGGAGCCAGCGCCATGCCCCCGGTCAATTCCGCAATGCGCGGTGCCAACGTTGAGGAGCGACGTg                                                   >  8:61460/1‑135 (MQ=255)
                                                                          cACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTCTTCACAGAAAGAGGAGCACGCGCCCAGCCCCCGGTGAATTCCGCCAAGCCCGGTGCCCACTTTGAGGAGCGCCggg                                                   >  4:182699/1‑133 (MQ=255)
                                                                             tGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGGTGTTCACCTAGAGGGGGCCCACCGCCCCGCCCCCGGGGAAATCCCCAATGCGCGGGGCCAACATTTAGGGGCGCCggggcc                                                >  5:287573/1‑130 (MQ=255)
                                                                               cTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGGTTATTGACCTACTGAGGAGCCAGCCCCAATCCCCCCGTCAATGCCGCAAAGCGCTGTGGCAACATTTAGTGGCGACCTGACCgg                                              >  8:115112/1‑135 (MQ=255)
                                                                                            tCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTGTTCACCTACTGAGGAGCAAGCGCCATGCCCCCCGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGACCGGGCCATGGCATTGt                                 >  7:104184/1‑135 (MQ=255)
                                                                                            tCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTGTTAACCTACTGAGGAGCAAGCGCCATGCCCCCCGGCAAAGCCGCAATGCGCTGTGCCCACTTTGAGGAGCGACGTGGCCCGGCCCTGGCATTGt                                 >  7:127139/1‑135 (MQ=255)
                                                                                                   cggcTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGACCGGGCCATGGCATTGTTTGCCGa                          <  3:222300/135‑1 (MQ=255)
                                                                                                   cggcTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGACCGGGCCATGGCATTGTTTGCCGa                          <  2:159980/135‑1 (MQ=255)
                                                                                                          ctggGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGACCGGGCCATGGCATTGTTTGCCGAAAAgg                     <  1:25608/133‑1 (MQ=255)
                                                                                                             gggCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGACCGGGCCATGGCATTGTTTGCCGAAAAGGGCttt                <  3:61546/135‑1 (MQ=255)
                                                                                                                  cAAGTGCGGTCGCGGTTTCTTCACATACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGATCGACGTGACCGGGCGATGGCATTGTTTGCCGAAATGGGCTTTGGCCa           <  7:386759/135‑1 (MQ=255)
                                                                                                                           tCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGACCGGGCCATGGCATTGTTTGCCGAAAAGGGCTTTGGCCAGGTCAGCAt  <  8:248055/135‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                
CGGCCTGGACACCCTGCTGGCAATCATGGAGGCCTTCCACGAAGGCTTCAACGACAGCAAATACCGCCCTGCTCCACTGCTCAAGGAAATGGTCGCGGCCGGCTGGCTGGGGCGCAAGAGCGGTCGCGGTTTCTTCACCTACTGAGGAGCAAGCGCCATGCCCCCGGTCAATGCCGCAATGCGCTGTGCCAACTTTGAGGAGCGACGTGACCGGGCCATGGCATTGTTTGCCGAAAAGGGCTTTGGCCAGGTCAGCAT  >  ACB122/4276914‑4277171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑