Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,874,7090AC53.1% 4.6 / 18.1 32intergenic (+13/‑174)CJ019_04568/uacT_1hypothetical protein/Uric acid transporter UacT
Reads supporting (aligned to +/- strand):  ref base A (7/8);  new base C (10/7);  total (17/15)
Fisher's exact test for biased strand distribution p-value = 7.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.22e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCT  >  ACB122/4874578‑4874840
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gcATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGCTCGCCAAAGGCGCCCAGGTCAACTGACAGCCCCACGCCCGGc                                                                                                                                  >  3:290054/1‑135 (MQ=255)
 cATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCa                                                                                                                                 <  2:239971/135‑1 (MQ=255)
  aTTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCaa                                                                                                                                >  6:202743/1‑135 (MQ=255)
          gcAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGCTCGCCCAAGGCGCCCACGGCCACTGACAGCCCCTCGCCCGGCACCCCGcccc                                                                                                                        >  1:103474/1‑135 (MQ=255)
                      cagcccgATAATCCCGCTGGGAAACATAACCCTGATGACCACCGATTACCCCACGCGCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGACCCCCAAGGGAATc                                                                                                             <  1:27053/134‑1 (MQ=255)
                             aTCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCACGGGAATCACCttgc                                                                                                      >  2:318470/1‑132 (MQ=255)
                                     gCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCCCCCCCCCAGGGAATCACCTTCCCCGGCAGGa                                                                                             >  2:260216/1‑135 (MQ=255)
                                         ggCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGCCAGCACCTCGCCACGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCgg                                                                                         >  2:92742/1‑135 (MQ=255)
                                                 cACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAACGGAATCACCTTCCCCTGCAGGCGCGGGCGTGccc                                                                                 >  2:257317/1‑135 (MQ=255)
                                                      aGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACAGGATCGCCAAACGCGCCCACGTCAACTGACAGCACCTCGCCCGGCCACCCGCCCCCCCACGGCATCACCTTCCCCAGCCGGCGCGGGCGTGCCCCCGaa                                                                            >  1:137867/1‑135 (MQ=255)
                                                                cGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCACGCCCGGCAACCCGCCCCCCCAGGGAATCCCCTTCCCCGGCAGGAGCGGGCGTGCCCGCGCAGAGGCCGGTa                                                                  >  5:173563/1‑135 (MQ=255)
                                                                cGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACATCCCCACGAAACCCGCCCCCCAAGGGCATCACAGTCCCCTGCAGGAGCGGGCTTGCCCCCGAAGATGCCGATa                                                                  >  2:378091/1‑135 (MQ=255)
                                                                   agagCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCAAGGGAATCACCGTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGCTAGcc                                                               >  2:71441/1‑135 (MQ=255)
                                                                   accccccAAGCTCGGCGGCGACTGGAGCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCACGGGAACCACCTGCCCCGGCAGGAGCGGGCTTGCCCGCGGAGAGGCCGATAGcc                                                               <  4:275722/129‑1 (MQ=255)
                                                                     agCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCCCCTCGCCACGCCAACCGCCCCCCCACGGAATGACCTTCCCCTGCCGGAGCGGGCGTGCCCGCGAAGAGGCCGATAGcccc                                                             >  7:173700/1‑135 (MQ=255)
                                                                       cgcACGCGCGGCGGCGACGGGATCGCCAACGGCGCCCAGGTCAACTGACAGCACCTCCCCCGGCAACCCGCCCCCCACGGGAAGCACCTTCCCCTGCAGGAGCGGGCTGGCCCGCGAAGAGGCCGATAGCCCCAg                                                           <  8:84690/135‑1 (MQ=255)
                                                                          aaGCTCGTCGGCGACTGGGTAGCCAAAGGCGCCCAGGGGAAATGACAGCCCCTAGCCCGGCAACCCGCCCCCCCAGGGCAGCACCTTCCCCTGGCGGAGCGGGCGTGGCCGCGCAGAGGCCGCTAGcccccccc                                                         >  2:94528/1‑130 (MQ=255)
                                                                          aaGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGCTAGcccccccct                                                        >  8:386909/1‑130 (MQ=255)
                                                                               gggcgGCCAGTGGAGCGCAACCGGCGCCCCGGAACACTGACACCCCCCCGCCCCCCACCCCCCCCCCCAGGGTAATCTCCTCCCCCGGCGGGCGGGGGGGTCCCCGCCACGAGGCCGATAGCCCCAGCCTCAAGc                                                   <  2:373312/132‑1 (MQ=255)
                                                                                    gCGACTGGCTCGCCAAAGGCGCCCAGGTCAACTGACAGCACCACGCCCGGCCACCCGCCCCCCCAGGGCATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGCAGAGGCCGATAGCCCCAGCCGCAAGCTccct                                              >  7:84690/1‑133 (MQ=255)
                                                                                            agcGCCAACGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACg                                      <  7:14544/133‑1 (MQ=255)
                                                                                                          ccAGGTCAACTCACACCACCTCGCCCGGCACCCCGCCCCCCAAGGGAAGCCCCTTCCCCTGCCGGAGCGGGCTGGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCAt                        <  3:69286/135‑1 (MQ=255)
                                                                                                           cAGGTCAACTGACCCCACCCCCCCCGGCACCCCCCCCCCCACGGGAATCACCTCCCCCTGCCGGCGCGGGCTGGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATc                       <  5:202743/135‑1 (MQ=255)
                                                                                                               tCAACTGACAGCCCCTCGCCAGGCACCCCCCGACCCACGTGAATCACATTCCCCTGCTGGAGCGTGGTTGCCCGCGATGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc                   <  8:92241/135‑1 (MQ=255)
                                                                                                               tCAACTGACAGCCCCGCGCCAGGCACCCCGCCCCCCACGGGAATCACCTCCCCCTGCCGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc                   <  7:333437/135‑1 (MQ=255)
                                                                                                               tCAACTCACAGCACCTCGCCCGGCACCCCGCCCCCCACGGGAATCACCTCCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc                   <  6:173563/135‑1 (MQ=39)
                                                                                                               tCAACTCACAGCACCTCGCCACGCAACCCCCCCCCCATGGGAATCCCCTTCCCCTGCAGGCGCGGGCTGCCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc                   <  2:291643/135‑1 (MQ=255)
                                                                                                                 acctcacacCACCTCGCCCGCCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCCGGCGCGGGCTTCCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgcgc                 <  7:323890/130‑1 (MQ=255)
                                                                                                                 aaCTGACAGCACCTCGACAGGCACCCCGCCCCCCAAGGGATTCACGTTCCGCTGCTGGAGCGGGCTTGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgcgc                 <  4:118352/135‑1 (MQ=255)
                                                                                                                            ccTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCATGCAGGAGCGCGCTTGCCCGCGAAGAGGCCGATCGCCCCAGCCTCAAGCTCCAGGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGaa      >  7:330324/1‑135 (MQ=255)
                                                                                                                              gcGCCCGGCACCCCGCCCCCCACGGGAACCACCTGCCCCTGCCGGCGCGGGCTGGCCCGCGACGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGc    <  1:77428/134‑1 (MQ=255)
                                                                                                                                gCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGTGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCt  >  4:121393/1‑135 (MQ=255)
                                                                                                                                   |                                                                                                                                   
GCATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCATGAAGCCT  >  ACB122/4874578‑4874840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑