| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 4,954,912 | 0 | T | G | 54.5% | 6.1 / 11.2 | 11 | intergenic (‑129/+135) | fliE/atoC_3 | Flagellar hook‑basal body complex protein FliE/Regulatory protein AtoC |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (0/6); total (0/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTCATGACCCTTCTCTCTCCTGCGGCCGCATTTTTTTGACAGTCATGCGGCGAATGACCTACCTAAAGCAACAAAGGTGCCAGCCTTCGCAACTCATTTCCCAGAGATCACACAATTCCTGCGGGAGCGGCCTTGCGTCGCGATAGGGCCGCAGAGCGGCCCCGGCGATCTTTGCGGCGGAGCTGAAGACCTGGGGCCGCTTCGCGCCCCATTCGCGACACAAGGCCGTTCCCACAGAGGTCCGCGCCAGTTGCAGATGAT > ACB122/4954779‑4955039 | cTCATGACCCTTCTCTCTCCTGCGGCCGCATTTTTTTGACAGTCATGCGGCGAATGACCTACCTAAAGCAACAAAGGTGCCAGCCTTCGCAACTCATTTCCCAGAGATCACACAATTCCTGCGGGAGCGGCCTTg < 4:223077/135‑1 (MQ=255)cTCATGACCCTTCTCTCTCCTGCGGCCGCATTTTTTTGACAGTCATGCGGCGAATGACCTACCTAAAGCAACAAAGGTGCCAGCCTTCGCAACTCATTTCCCAGAGATCACACAATTCCTGCGGGAGCGGCCTTg < 8:295872/135‑1 (MQ=255) gaaccTTCTCTCTCCTGCGGCCGCATTTTTTAGACAGTCATGCGGCGAATGACCTACCTAAAGCAACAAAGGTGCCACCCTTCTCAACTCATTTCCCAGAGATCACACAATTCCTGCGGGAGCGGCCTTGCGTcg < 7:298736/132‑1 (MQ=255) cccTTCTCTCTCCTGCGGCCGCATTTTTTTGACAGTCATGCGGCGAATGACCTACCTAAAGCAACAAAGGTGCCAGCCTTCGCAACTCATTTCCCAGAGATCACACAATTCCTGCGGGAGCGGCCTTGCGTcgcg < 6:163654/135‑1 (MQ=255) ccTTCTCTCTCCTGCGGCCGCATTTTTTTGACAGTCATGCGGCGAATGACCTACCTAAAGCAACAAAGGTGCCAGCCTTCGCAACTCATTTCCCAGAGATCACACAATTCCTGCGGGAGCGGCCTTGCGTCGCGa < 3:248240/135‑1 (MQ=255) gccaggcggcaAAGCACCAACCAAACCCAACACCGGGGCCCGCCTTCGCAACTCATACCCCCCAGACCACACAATGCCTGCGGGCGGGGCCTGGCGCCGCGATCGGGCCGCAGCGCGCCCCCGGCGATCTTTgcg < 4:151210/130‑1 (MQ=255) ctaccaaACCCAACAACGGGCCCACCCTTCGCAACTAATTACCCACACAACACACAATTCCTGCGGGCGCGGCCTGGCGGCGCTATCGGGCCGCAGCGCGCCCCCGGCGATCTTTGCGGCGGAGCTGAAGACCTg < 6:324339/135‑1 (MQ=255) cccTACGAAACTCTTTACCCTCATATCACACAATCCGGGCGGGCGGGGCGTGGCGGGGCTATGGGGCGGCTGCGCGGCCCCGGCGATCTTTGCGGCGGAGCTGAAGACCTGGGGCCGCTTCGCGCCCCATTcgcg < 8:39738/134‑1 (MQ=255) ccAACACATTCCCCCCAGAACCCACAATCCCGGCGGCCGCGGCCTGGCGGCGCGATAGGGCCCCAGCGCGCCCCCGGCGATCTTTGCGGCGGAGCTGAAGACCTGGGGCCGCTTCGCGCCCCATTCGCGACACaa < 4:177609/134‑1 (MQ=25) cccACAGAACACAAAATCCGGGCGGGCGCGGCCGGGCGCCCCTATTGCCCCGCCGCGCGCCCCCGGCGATCTTTGCGGCGGAGCTGAAGACCTGGGGCCGCTTCGCGCCCCATTCGCGACACAAGGCCGTTCCca < 6:151229/135‑1 (MQ=16) ggCCTGGCGTCGCTATCGGGCGCCAGAGCGGCCCCGGCGATCTTTGCGGCGGAGCGGAAGACCTGGGGCCGCTTCGCGCCCCATTCGCGACACAAGGCCGTTCCCACAGAGGTCCGCGCCAGTTGCAgatgat < 2:294696/133‑1 (MQ=37) | CTCATGACCCTTCTCTCTCCTGCGGCCGCATTTTTTTGACAGTCATGCGGCGAATGACCTACCTAAAGCAACAAAGGTGCCAGCCTTCGCAACTCATTTCCCAGAGATCACACAATTCCTGCGGGAGCGGCCTTGCGTCGCGATAGGGCCGCAGAGCGGCCCCGGCGATCTTTGCGGCGGAGCTGAAGACCTGGGGCCGCTTCGCGCCCCATTCGCGACACAAGGCCGTTCCCACAGAGGTCCGCGCCAGTTGCAGATGAT > ACB122/4954779‑4955039 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |