Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,974,0620TG57.1% 1.0 / 12.2 28L684R (CTG→CGG) sasA_19Adaptive‑response sensory‑kinase SasA
Reads supporting (aligned to +/- strand):  ref base T (3/6);  new base G (4/8);  total (14/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.02e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AAGCCTTGATTGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCTGGC‑CATGCGCCAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAA  >  ACB122/5973930‑5974192
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aaGCCTTGATTGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCTgg                                                                                                                                   <  8:28215/135‑1 (MQ=255)
       gATTGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCTGGC‑CAAgcg                                                                                                                           >  4:90999/1‑135 (MQ=255)
       gATTGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCAGGC‑CATgcg                                                                                                                           >  8:61248/1‑135 (MQ=255)
         ttGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCGGGG‑CCTTCGcc                                                                                                                         >  8:250831/1‑135 (MQ=255)
         ttGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCGGGC‑CCAGCGcc                                                                                                                         >  8:370080/1‑135 (MQ=255)
                     tCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACACATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCTGGC‑CCTGCGCCCGGCGTGGcccg                                                                                                             >  1:101400/1‑133 (MQ=255)
                              cGGGCCAGAAGGGCTTGGGCTTCACTCATGACCGGGGGAGGGAGGCCGCTTAGGCACTCACGCCCGTGCACCCGCGCGCCTCGCGGCAGGCC‑CGTATCGCCCTGGC‑CATGCGCCCGGCTTGGCCCGGGCGAACGc                                                                                                    <  8:98856/135‑1 (MQ=255)
                                              gggCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCAGGCGCAT‑CGCCAGGCTTTGCCAGGGCGGACGCACGAGGGGTTTTTGGa                                                                                    >  1:38624/1‑135 (MQ=255)
                                              gggCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCAGGC‑CCTGCCCCCCCCATGGCCAGGGCGCACGCCCCAGGGGTTGTTCGa                                                                                    >  8:267051/1‑135 (MQ=255)
                                                     aCTCATGACGGGGGGGGGGCGGCGGCTTCGCCCCCCCCGCCCGGGCCCCCGCGCGCCGCGCGCCCTGCC‑CGACTCGCCCGGCC‑CAGCCGCCCGGGTGGCCCCGGGCGAACGCACGAGGTGTTGTTCGAGCTCgcc                                                                             <  2:116384/135‑1 (MQ=255)
                                                      cacATCACGGGGTGAGGGGGGCCGTTTATGCACTCACGCCGGTGCACCCGCGGGCCTCGCTGCATGCA‑GGTATCCCCCGGCC‑CAGGCGCCTGGGTGGGCCTGGGCGAACGCACGAGGTGTTGTTCGAGCTCgcc                                                                             <  2:38624/132‑1 (MQ=255)
                                                       tCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCAGGC‑CAAGCGCCCGGCGTGGGCCGGGCGCACGCACGAGGGGGTGTTGGAGCTCgcccc                                                                           >  2:277423/1‑133 (MQ=255)
                                                        cATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGTGCCTCGCTGCATGCA‑CGTATCGCCCAGGC‑CCAGCGCCAGGCTTTGCCAGGGGGCCCGCCCGCGGGGTTGTTAGCGCGAgccagc                                                                          >  4:151695/1‑135 (MQ=255)
                                                         ttccccGGGTGAGGGCGGCGGCTTAGGCACCCCCGCCCGTGCACCAGCGCGCCGCGCTGCAGGCA‑CGTAGCGCCCGGCC‑CAGGCGCCTGGCTGGGCCTGGGCGAACGCACGAGGTGTTGTTCGAGCTCgccagcc                                                                         <  6:63962/131‑1 (MQ=255)
                                                            aCCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCATGC‑CATGCGCCACGCAAGGCCCCGGCGCCCCCACCCCCGGTTGTTGGAGCACGCcagccagc                                                                      >  1:145735/1‑135 (MQ=255)
                                                             cgggggGAGGGCGGCGGCTCATCCACACCCGCCCGGCCACCCGCGCGCCTCGCGGCAGGCC‑CGTAGCGCCCGGCC‑CAGGCGCCCGGCGTGGCCCGGGCGAACGCACGAGGTGTTGGTCGAGCTCGCCAGCCAGCt                                                                     <  3:81472/133‑1 (MQ=255)
                                                                    aTGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCCGGC‑CCAGCGCCCCGCGTGGGCAGGGCGCACGCCCGCGGTGGTTTTCGAGCACGCCCCCCCCCTACAAccc                                                              >  8:352828/1‑133 (MQ=255)
                                                                              cTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCTGGC‑CCTGCGCCAGGCGTGGCCCGGGCGGACGCCCGCGGTGTTGGTCGAGCTCGCCAGCCCGCGACAACGCGCCCGccccg                                                    >  2:232161/1‑134 (MQ=255)
                                                                                    cacgcccGCCGGTCCACCCGCGCGCCCCGCGGCATGCA‑GGTAGCGCCCTGGC‑CATGCCCCAGGCTGGGCCGGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGt                                              <  7:187883/129‑1 (MQ=255)
                                                                                    cacccccGCCCGGCCACCCGCGCGCCGCGCTGCA‑GCACCGTATCGCCGGGCC‑CAGGCGCCCGGGTGGGCCGGGGCGAACGCACGAGGTGTTGGTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGt                                              <  4:144076/129‑1 (MQ=255)
                                                                                          cGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCGCGC‑CATGCGCCCGGCGTGGGCCCCGCGCCCGCCCGACGGGTTGTTTGCGCGCGCCCCCCCCCGAACACCCGCCCCCCCCTGCGGGGTTGCAg                                        >  2:317948/1‑135 (MQ=255)
                                                                                            cccGTGCACCCGCGCGCCGCGCGGCATGCA‑CGTAGCGCCCTGCC‑CATCCGCCCGGCTGGGCCCGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCg                                      <  6:119323/135‑1 (MQ=255)
                                                                                            cccGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCGGGG‑CCAGCCCCCCGCGTGGGCCGGGGGCACGCCCCGGGGGGTGTTGGAGCTCGGCCGCCCCCAACAACCCGCCCGCCCCGGCGGGGTTTGAGCg                                      >  8:187883/1‑135 (MQ=255)
                                                                                                      aGCGCGCCTCGCTGCTTGCA‑CGTAGCGCCCGGCC‑CAGGCGCCAGGCGTGGCCTGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCg                            <  3:90999/135‑1 (MQ=255)
                                                                                                              tCGGTGAATGCA‑CGTAGCGCCCGGCC‑CATGCGCCGGGCTGGGCCCGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTc                    <  7:352828/135‑1 (MQ=255)
                                                                                                                            gTATCGCCCGGGC‑CAGGCGCCAGGCGGGGCCGGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTAc       <  7:190453/135‑1 (MQ=255)
                                                                                                                                cccccTGGC‑CATGCGCCTGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGa   <  2:290142/133‑1 (MQ=255)
                                                                                                                                 gCCCTGGC‑CATGCGCCAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGaa  <  4:292404/135‑1 (MQ=255)
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AAGCCTTGATTGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCA‑CGTATCGCCCTGGC‑CATGCGCCAGGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAA  >  ACB122/5973930‑5974192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑