Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB122	1,806,928	G→T	G76V (GGG→GTG)	yeiG →	S‑formylglutathione hydrolase YeiG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,806,928	0	G	T	89.0%	182.3 / 13.1	73	G76V (GGG→GTG)	yeiG	S‑formylglutathione hydrolase YeiG
Reads supporting (aligned to +/- strand):  ref base G (5/3);  new base T (34/31);  total (39/34)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.88e-01

CGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTGGGCCCAGCACT  >  ACB122/1806797‑1807059                                                                                                                                    |                                                                                                                                    cGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGGGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCt                                                                                                                                  <  4:7551/135‑1 (MQ=255)  gCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGcgg                                                                                                                                 >  3:8441/1‑133 (MQ=255)  gCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTg                                                                                                                                 >  2:99256/1‑135 (MQ=255)  gCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTg                                                                                                                                 >  3:133291/1‑135 (MQ=255)  gCTGTGTACCTGCCGCCGCAGGACGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACAGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGcgg                                                                                                                                 >  5:26534/1‑133 (MQ=255)         aCCTGCCGCGGCTGGCGTAGCAGGTCGAAAAGCTGCCGTTTCTTTTCTGGCTCAGCGGCCTAACCTCCACCGACGAGAACTTCATGCCGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCAtcg                                                                                                                          <  4:295437/135‑1 (MQ=255)         aCCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGatcatc                                                                                                                           <  3:168190/134‑1 (MQ=255)         aCCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCAtcg                                                                                                                          <  6:383323/135‑1 (MQ=255)               cgccgcAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGc                                                                                                                      <  1:310561/133‑1 (MQ=255)               cgccgcAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGc                                                                                                                      <  7:191859/133‑1 (MQ=255)               cgccgcAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGc                                                                                                                      <  7:94209/133‑1 (MQ=255)                gccgcAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGCCGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGcccc                                                                                                                   >  6:313/1‑135 (MQ=255)                 ccgcAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGccccc                                                                                                                  >  4:232327/1‑135 (MQ=255)                 ccgcAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGcccc                                                                                                                   >  8:198607/1‑134 (MQ=255)                 ccgcAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGcccc                                                                                                                   >  1:298186/1‑134 (MQ=255)                   gcAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCg                                                                                                                 >  3:18025/1‑134 (MQ=255)                     cggCCGAGCAGGGCGAAAAGCTGCCGGTGCTCTACTCGCTCAGCGGCATCACCTGCACCGACGAGAAATTCATTCAGAATGCCGGCGCCCAGCGTCTGTCCGCCGAGCTGGTGCTCCTCATCGTCGCCCCCGATa                                                                                                              >  4:321928/2‑135 (MQ=255)                                   gAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGc                                                                                                >  3:23957/1‑135 (MQ=255)                                    aaaaGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCg                                                                                               <  7:277304/135‑1 (MQ=255)                                    aaaaGCTGCCGGGGCTGTACTGGCGCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCg                                                                                               <  6:332131/135‑1 (MQ=255)                                        gCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCCGCCCGCGTGGCGAGCa                                                                                           >  2:249706/1‑135 (MQ=255)                                             cGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGatcatc                                                                                                                           >  8:179958/1‑98 (MQ=255)                                             cGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGatcatc                                                                                                                           <  7:179958/98‑1 (MQ=255)                                               gTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCg                                                                                    >  1:85499/1‑135 (MQ=255)                                                        tGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCg                                                                           <  3:175097/135‑1 (MQ=255)                                                           cTCAGCGGCCTCACCTCCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCgg                                                                          <  4:240738/133‑1 (MQ=255)                                                             cAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGCGCAGGTGCCGGGCGCTCCGGACgg                                                                      >  1:337256/1‑135 (MQ=255)                                                             cAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACgg                                                                      >  7:107103/1‑135 (MQ=255)                                                             cAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACgg                                                                      >  1:28511/1‑135 (MQ=255)                                                             cAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACgg                                                                      >  1:200770/1‑135 (MQ=255)                                                             cAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACgg                                                                      >  4:220240/1‑135 (MQ=255)                                                                  gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCAtcgtc                                                                                                                        <  2:294205/80‑1 (MQ=255)                                                                  gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCAtcgtc                                                                                                                        >  1:294205/1‑80 (MQ=255)                                                                  gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGc                                                                                                                      <  3:251034/82‑1 (MQ=255)                                                                  gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGc                                                                                                                      >  4:251034/1‑82 (MQ=255)                                                                  gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGCCGGCGCCt                                                                 >  5:100547/1‑135 (MQ=255)                                                                  gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCt                                                                 >  4:6414/1‑135 (MQ=255)                                                                  gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCt                                                                 >  4:25078/1‑135 (MQ=255)                                                                  gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCCTGGCGAGCAGGTGCCGGGCGCTCCGGACGGCGccg                                                                 >  7:164397/1‑134 (MQ=255)                                                                  gCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGAGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCt                                                                 >  8:257519/1‑135 (MQ=255)                                                                        ccTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACt                                                           >  3:271910/1‑135 (MQ=255)                                                                               cgacgaGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCt                                                    >  6:32156/1‑135 (MQ=255)                                                                                gacgaGAACTTCATGCAGAAGGACGGCGCCCAGCGTCTGGCCGCCGAACAGGTGCAGATAATCGTCGCCCCCGATACACGCCCGCGTCGCGCGCAGGTGCCGGGCGATCCGGACGGCCCCTGGGACGTCGGCCTg                                                   >  6:243676/1‑135 (MQ=255)                                                                                 acgaGAACTTCATGCAGACGGCCGGCGCCCAGGGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTgg                                                  <  6:36424/135‑1 (MQ=255)                                                                                  cgaGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTggg                                                 >  1:110904/1‑135 (MQ=255)                                                                                    agaACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGgcg                                               >  8:34916/1‑135 (MQ=255)                                                                                    agaACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGgcg                                               >  1:22260/1‑135 (MQ=255)                                                                                    agaACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGGGGCGCGCAGGTGCCGGGCGCTCCGGACGGCGCCGGGGCCTTCGGCCAGGgcg                                               >  2:198148/1‑135 (MQ=255)                                                                                        cTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGGCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTgg                                           <  6:313100/135‑1 (MQ=255)                                                                                         ttCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGc                                          <  5:32156/135‑1 (MQ=255)                                                                                         ttCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGc                                          <  7:125605/135‑1 (MQ=255)                                                                                         ttCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGc                                          >  8:6152/1‑135 (MQ=255)                                                                                                   aGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCa                                >  6:2711/1‑135 (MQ=255)                                                                                                      ccGGCGCCCAGCGTCTGGCCGGCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGGAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACg                             <  2:270643/135‑1 (MQ=255)                                                                                                      ccGGCGCCCAGCGGCTGGCCGCCGATCGGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACg                             <  6:50905/135‑1 (MQ=255)                                                                                                           gcCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCAcc                        <  2:22260/135‑1 (MQ=255)                                                                                                           gcCCAGCGTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCAcc                        <  7:34916/135‑1 (MQ=255)                                                                                                             ccAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCa                      >  5:357832/1‑135 (MQ=255)                                                                                                                  gTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAAc                 >  8:195961/1‑135 (MQ=255)                                                                                                                  gTCTGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAAc                 <  2:85499/135‑1 (MQ=255)                                                                                                                  gTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAAc                 <  6:357832/135‑1 (MQ=255)                                                                                                                     tGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCt              <  1:99256/135‑1 (MQ=255)                                                                                                                     tGGCCGCCGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCt              <  3:110640/135‑1 (MQ=255)                                                                                                                       gccgccGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTgg            <  4:18025/135‑1 (MQ=255)                                                                                                                       gccgccGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTgg            <  3:25078/135‑1 (MQ=255)                                                                                                                       gccgccGAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTgg            <  8:107103/135‑1 (MQ=255)                                                                                                                        ccgccgAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTgg            <  7:257519/134‑1 (MQ=255)                                                                                                                         cgccgAGCTGGTGCTGATCATCGTCGCCCCCGATACGAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCG‑CTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTGGGcc         <  2:298186/135‑1 (MQ=255)                                                                                                                             gAGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTGGGCCCAg      <  3:6414/135‑1 (MQ=255)                                                                                                                              aGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGgcgc                                                                   <  5:265835/73‑1 (MQ=255)                                                                                                                              aGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGgcgc                                                                   >  6:265835/1‑73 (MQ=255)                                                                                                                              aGCTGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACATCAACGCCACCCCGCCACCCGGGGCCCAGc     >  1:111092/1‑135 (MQ=255)                                                                                                                                 tGGTGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTGGGCCCAGCACt  <  7:195961/135‑1 (MQ=255)                                                                                                                                    |                                                                                                                                    CGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGACGAGAACTTCATGCAGAAGGCCGGCGCCCAGCGTCTGGCCGCCGAGCTGGGGCTGATCATCGTCGCCCCCGATACCAGCCCGCGTGGCGAGCAGGTGCCGGGCGATCCGGACGGCGCCTGGGACTTCGGCCTGGGCGCTGGCTTCTACCTCAACGCCACCCAGCAACCCTGGGCCCAGCACT  >  ACB122/1806797‑1807059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑