| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 5,688,634 | A→C | 35.3% | T235P (ACC→CCC) | pyrC' → | Dihydroorotase‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 5,688,634 | 0 | A | C | 35.3% | 15.9 / 3.9 | 40 | T235P (ACC→CCC) | pyrC' | Dihydroorotase‑like protein |
| Reads supporting (aligned to +/- strand): ref base A (11/14); new base C (2/13); total (13/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.01e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCC > ACB122/5688503‑5688750 | tGGCCCACGAAGGTGCCATGGCCCGCTTCCTCGGCCTGCCGGGCATCCCGGAGCGCGCCGAAACCGTGGCCCTGGCGCGCACCCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCACGTCAGCCCGCTAACCa > 6:145817/1‑135 (MQ=255) aCGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGGGCCCGTCAGCCCCCTCACCACCGccc > 3:314060/1‑135 (MQ=255) aGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCCCCTCCGCCCGATAACCAGCGCCCGTgg > 2:126442/1‑135 (MQ=255) tGGCCAGCTTCCTCGGCCGGCCGGGCATCCCGGAGCGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGGGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGc < 3:382744/135‑1 (MQ=255) aGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCCCTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCGGATCg > 7:212660/1‑136 (MQ=255) ccTCGGCCTGCCGGGCATCCAGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGT‑GAACAGAGCGGCGTGGGGGGGCACGTGCGCCATAACCCCCCAGCCCCTGGCGCCCCGCGGATCgccccg > 6:70234/1‑133 (MQ=255) tCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCGGCGGGTGGAACAGAGCGGCGGGCGTGCGCACTTCCGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCgcccagg < 3:186788/135‑1 (MQ=255) ggCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCCCGTCCCCCACATCACCCCCGCCCGGGGCGCCCGGGGGGTCgccccggcc > 2:246654/1‑135 (MQ=255) ccGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGGGGGCCCTGGAGCCACACCCCCACCGCCCCGGGCGCGCGGGGGCTCgcccccgcccccgcc > 3:253619/1‑130 (MQ=255) gcagagCGCCTAAACGGGGGCCCGGGCGCGAAACCTGGTGCTGGTGGAACCGAGCGGGGTGCGTGCGCACTTCCGCCAGATCCCCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGcc < 3:123163/133‑1 (MQ=255) gcgcCGAACCGGGGGCCGGGGGGCGCACCGTGGGGGTGGTGGAACCGCGGGGGGGGGGGGCGCACTTCCGCCACAGCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc < 8:364602/136‑1 (MQ=255) gcgcCGAACCGGGGGCCCTGGCGCGCAACCGGCTGCTGGTGTAACCGAGCGGCGTGGGTGCGCACTTCAGCCCTAACCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc < 7:315396/136‑1 (MQ=255) gcCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCAGCCAGATCACCCGCCCCCCTGGCGCGCGGCTGCTCGCCCCCGCCCCGGCCGTGGGGCTGCCgggtacc > 1:32848/1‑130 (MQ=255) cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGGGGGGCCTGGAGCCACCTAACCCGCGCCCGGGGCGCGCGGCGGCTCGCCCCGGCCCCGGCCGTGGGGCGGCCGGGGAccgg > 5:187535/1‑134 (MQ=255) cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGGGGGGCCGTCAGCCAGATCAACCCCCCCCCGGGCGCGCCGCGGGACGCCCCCGCCCCGGGCGTGGGCCTGCCCGGAAcccc > 6:359857/1‑133 (MQ=255) cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGGGCACTTCAGCCCGAACACCCCCCCCCCTGGGGCGCGGCGGCTAGCCCCCGCCCACGCCGTGGGCCAGCCCGGAAccgc > 6:112585/1‑135 (MQ=255) aaaCCGGGGCCCTGGCGCGCAACCTGCTGCTGGGGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGAGCCCCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg < 3:53954/135‑1 (MQ=255) aaCGGTGGCCGTGGCGGGCAACCGGGTGCGGGTGCACCAGAGCGGCGGGCGGGCGCAGTTCAGCCAGACCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGa < 4:215227/135‑1 (MQ=255) gggggCCCGGGCGCGAAACCTGCGGGGGGGGCAACAGAGCGGCGGGCGGGCGCACTTCAGCCAGATCCCCAGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 7:257385/133‑1 (MQ=255) cggggCCCTGGCGCGCAACCTGCTGCGGGGGGAACAGAGCGGCGGGCGTGCGCACTTCCGCCACAGCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 8:370936/133‑1 (MQ=255) gTGGCCCTGGCGCGCAACCTGCTGCGGGGGGAACAGCGCGGCGTGCGTGCGCACTTCAGCCAGATCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 2:32848/135‑1 (MQ=255) gTGGCCCTGGCGCGCAACCGGGTGGGGGGGGAACTGAGGGGGGTGCGTGCGCACTGCTGCCAGAGCCCCGGCGCCCGTGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 4:314060/135‑1 (MQ=255) gcAACCTGGGGCTGGGGAACCAGCGCGGCGGGCGGGCGCACTTCCGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCt < 7:322633/136‑1 (MQ=255) gaaaCCTGCGGCTGGGGGAACCGGGCGGGGGGCGGGGGCACTTCTGCCAGAGCACCCGGGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGc < 1:246654/133‑1 (MQ=255) ccTGCTGCTGGTGGAACAGAGCGGCGTGCGAGCGCCCTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGCTCGCCCCGGCCCCGGCCGTGGGCCTGCCCGTAACCGCCGATGTAGCGCTGTACCAGCTGAt > 1:57948/1‑135 (MQ=255) cAGAGCGGCGTGCGTGCGCACATCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCCGGCCGTGGGCCTGCCCGTAACCCCCCATGTAGCGCTGTACCAGCTGCTACTCACGGCCGAGTCg > 8:319947/1‑135 (MQ=255) cgcggcgGGCGTGCGCACTTCCGCCAGAGCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTg < 1:3372/134‑1 (MQ=255) gcggcgTGCGGGCGCACTCCCCCCAGACCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg < 7:201757/136‑1 (MQ=255) gcggcgGGCGGGCGCACCCCCGCCACACCCCCGCCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg < 7:346479/136‑1 (MQ=255) cggcgTGCGTGCGCACTTCAGCCCCAGCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg < 2:57948/135‑1 (MQ=255) gggcgggcgCACTTCCGCCATATCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt < 7:319947/134‑1 (MQ=255) gggcgggcgCACTCCAGCCAGATCCCCAGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt < 8:79315/134‑1 (MQ=255) tgggggcgcACTCCAGCCAGACCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt < 5:112585/130‑1 (MQ=255) tgcgtgcgAACTGCAGCCAGAGCACCCGGGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt < 6:125761/135‑1 (MQ=255) ggcgggcgCACTCCCCCCAGAACACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt < 6:305021/134‑1 (MQ=255) gcgggcgCACTTCCGCCAGAGCACCCGCGCCCGTGCGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGtt < 4:127569/135‑1 (MQ=255) gcgggcgCACTCCCGCCAGACCACCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTtc < 8:366354/136‑1 (MQ=255) cgtgccAACTGCAGCCAGAGCCCCCGCGCCCGGGCCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTATCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTtc < 6:190352/135‑1 (MQ=255) cgggcgcACTTCAGCCACTTCACCCGGGCCGGTGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATGCTCACGGACGAGTCGCTGCGCGAGTtc < 6:304293/132‑1 (MQ=255) tgcgCAGTTCAGCCAGATCACCAGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc < 8:84320/136‑1 (MQ=255) | TGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCC > ACB122/5688503‑5688750 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |