Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,212,593 G→T 5.5% G312G (GGG→GGT rdmC_1 → Aclacinomycin methylesterase RdmC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,212,5930GT 5.5% 214.5 / 4.1 73G312G (GGG→GGTrdmC_1Aclacinomycin methylesterase RdmC
Reads supporting (aligned to +/- strand):  ref base G (30/39);  new base T (2/2);  total (32/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.97e-01

GGCACGGCTGATCCGTTATTGCCGGTGATGCATGGCGTGCACTTGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCC  >  ACB122/1212462‑1212720
                                                                                                                                   |                                                                                                                               
ggcacggcTGATCCGTTATTGCCGGTGATGCATGGCGTGCACTTGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCg                                                                                                                              <  4:241366/135‑1 (MQ=255)
    cggcTGATCCGTTATTGCCGGTGATGCATGGCGTGCACTTGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGgtgt                                                                                                                          >  1:129777/1‑135 (MQ=255)
    cggcTGATCCGTTATTGCCGGTGATGCATGGCGTGCACTTGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGgtgt                                                                                                                          >  2:396571/1‑135 (MQ=255)
     ggcTGATCCGTTATTGCCGGTGATGCATGGCGTGCACTTGGCGGCGCATATCCGGGGCTGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGtt                                                                                                                         >  2:287055/1‑135 (MQ=255)
     ggcTGATCCGTTATTGCCGGTGATGCATGGCGTGCACTTGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGtt                                                                                                                         >  3:84089/1‑135 (MQ=255)
     ggcTGATCCGTTATTGCCGGTGATGCATGGCGTGCACTTGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGtt                                                                                                                         >  4:191709/1‑135 (MQ=255)
     ggcTGATCCGTTATTGCCGGTGATGCATGGCGTGCACTTGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGtt                                                                                                                         >  6:175343/1‑135 (MQ=255)
     ggcTGATCCGTTATTGCCGGTGATGCATGGCGTGCACTTGGCGGCGCATATCCGGGGCAGCAAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTg                                                                                                                        >  7:364050/1‑136 (MQ=255)
        tGATCCGTTATTGCCGGTGATGCATGGCGTGCACTTGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGtt                                                                                                                         <  8:246951/132‑1 (MQ=255)
        tGATCCGTTATTGCCGGTGATGCATGGCGTGCACTTGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGtt                                                                                                                         >  7:246951/1‑132 (MQ=255)
        tGATCCGTTATTGCCGGTGATGCATGGCGTGCACTTGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGcc                                                                                                                      >  1:213306/1‑135 (MQ=255)
        tGATCCGTTATTGCCGGTGATGCATGGCGTGCACTTGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGcc                                                                                                                      >  5:200749/1‑135 (MQ=255)
          aTCCGTTATTGCCGGTGATGCATGGCGTGCACTTGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTa                                                                                                                   >  7:110854/1‑136 (MQ=255)
             cGTTATTGCCGGTGATGCATGGCGTGCACTTGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTAttt                                                                                                                <  8:110854/136‑1 (MQ=255)
                   tGCCGGTGATGCATGGCGTGCACTTGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGCTCCCGGGGCTGGCGCACCGTTTCGAGGAGCCGTTCAAGGCGCCGTTACGGGGGGCGGTGTTGCCTTTTTTGGAgg                                                                                                           >  1:188695/1‑135 (MQ=255)
                   tGCCGGTGATGCATGGCGTGCACTTGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTGCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAg                                                                                                            <  5:218513/134‑1 (MQ=255)
                    gCCGGTGATGCATGGCGTGCACTTGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGgc                                                                                                          >  5:74376/1‑135 (MQ=255)
                    gCCGGTGATGCATGGCGTGCACTTGGCGGCGCATATCCGGGACAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGgc                                                                                                          <  8:206222/135‑1 (MQ=255)
                     ccGGTGATGCATGGCGTGCACTTGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGgc                                                                                                          <  5:95326/134‑1 (MQ=255)
                     ccGGTGATGCATGGCGTGCACTTGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGgc                                                                                                          <  5:229717/134‑1 (MQ=255)
                            tGCATGGCGTGCACTTGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGc                                                                                                  <  4:213270/135‑1 (MQ=255)
                            tGCATGGCGTGCACTTGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGc                                                                                                  >  8:147639/1‑135 (MQ=255)
                                    gtggtcTTGGCGGCGCATATCCGGGTCAGGCAGTTGCGCTTGATCCCGGGGCTGGAGCAGCGTTTCCAGGAGCCGTTCAAGGCGGCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTc                                                                                          <  1:360205/130‑1 (MQ=255)
                                        aCTTGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGtt                                                                                                                         <  3:100478/100‑1 (MQ=255)
                                        aCTTGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGtt                                                                                                                         >  4:100478/1‑100 (MQ=255)
                                          ttGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGgc                                                                                                          >  6:71992/1‑113 (MQ=255)
                                          ttGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGgc                                                                                                          <  5:71992/113‑1 (MQ=255)
                                             gcggcgCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATc                                                                                 <  5:25252/135‑1 (MQ=255)
                                             gcggcgCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATc                                                                                 <  7:133522/135‑1 (MQ=255)
                                              cggcgCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATc                                                                                 <  1:162114/134‑1 (MQ=255)
                                               ggcgCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGgc                                                                                                          <  6:350380/108‑1 (MQ=255)
                                               ggcgCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGgc                                                                                                          >  5:350380/1‑108 (MQ=255)
                                               ggcgCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATc                                                                                 >  3:37540/1‑133 (MQ=255)
                                                    atatCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCGTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGct                                                                         >  7:288334/1‑136 (MQ=255)
                                                       tCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGctc                                                                        >  1:101458/1‑134 (MQ=255)
                                                        ccGGGGCAGCCAGTTGCGCTTGATCCAGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTg                                                                      <  4:231389/135‑1 (MQ=255)
                                                           gggCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATc                                                                  <  7:147639/136‑1 (MQ=255)
                                                              cAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCgg                                                                <  1:396571/135‑1 (MQ=255)
                                                              cAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCGTTTTTGCAGGCGCACCGGCAAGCGGTAACGCACATCGCCTGGCTCTGCATCgg                                                                >  2:198831/1‑135 (MQ=255)
                                                              cAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTGGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCgg                                                                <  6:288581/135‑1 (MQ=255)
                                                                       gcgcTTGATACCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTc                                                      <  8:319880/136‑1 (MQ=255)
                                                                        cgcTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTc                                                      <  1:203703/135‑1 (MQ=255)
                                                                         gcTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCg                                                     >  1:118716/1‑135 (MQ=255)
                                                                         gcTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCg                                                     <  3:145052/135‑1 (MQ=255)
                                                                               tCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGt                                               <  2:77540/135‑1 (MQ=255)
                                                                                cccGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGTGCGGTGTTGCCTTATTTGCAgg                                                                                                           <  5:174752/74‑1 (MQ=255)
                                                                                cccGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGTGCGGTGTTGCCTTATTTGCAgg                                                                                                           <  5:181301/74‑1 (MQ=255)
                                                                                cccGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGTGCGGTGTTGCCTTATTTGCAgg                                                                                                           >  6:174752/1‑74 (MQ=255)
                                                                                cccGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGTGCGGTGTTGCCTTATTTGCAgg                                                                                                           >  6:181301/1‑74 (MQ=255)
                                                                                 ccGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGc                                             >  6:74029/1‑135 (MQ=255)
                                                                                   ggggCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCAt                                           <  2:101458/135‑1 (MQ=255)
                                                                                   ggggCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCAt                                           <  6:166967/135‑1 (MQ=255)
                                                                                   ggggCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCAt                                           <  1:49603/135‑1 (MQ=255)
                                                                                   ggggCTGGCGCACCGGTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCAt                                           <  5:180682/135‑1 (MQ=255)
                                                                                       cTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGc                                       >  6:154260/1‑135 (MQ=255)
                                                                                           cgcACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCAccc                                   <  4:378189/135‑1 (MQ=255)
                                                                                             cACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCcac                                 <  2:352641/135‑1 (MQ=255)
                                                                                                cGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCcacaca                              <  2:240469/135‑1 (MQ=255)
                                                                                                   ttCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCAccc                                   >  1:340527/1‑127 (MQ=255)
                                                                                                   ttCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCAccc                                   <  2:340527/127‑1 (MQ=255)
                                                                                                             cGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCACCACgg                 >  1:247417/1‑135 (MQ=255)
                                                                                                              gTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGt                <  3:191709/135‑1 (MQ=255)
                                                                                                               ttCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTAGCTCAGTGCATACGCCCCCACACCGTCCCCAGCAccgga               >  1:326942/1‑131 (MQ=255)
                                                                                                                 cAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTcgc             >  6:186681/1‑135 (MQ=255)
                                                                                                                  aaGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGgc                                                                                                          <  3:322897/41‑1 (MQ=255)
                                                                                                                  aaGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGgc                                                                                                          >  4:322897/1‑41 (MQ=255)
                                                                                                                   aGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTcgcg            <  6:165076/134‑1 (MQ=255)
                                                                                                                       gcCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACTCTGTCACCAGCACGGTCGCGGCTATc      <  8:54485/136‑1 (MQ=255)
                                                                                                                        ccGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATc      <  5:175343/135‑1 (MQ=255)
                                                                                                                           ttACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGCTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGcc  <  8:364050/136‑1 (MQ=255)
                                                                                                                            tACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTc                                                      <  6:287530/83‑1 (MQ=255)
                                                                                                                            tACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTc                                                      >  5:287530/1‑83 (MQ=255)
                                                                                                                            tACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCAACCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGcc  <  1:287055/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                               
GGCACGGCTGATCCGTTATTGCCGGTGATGCATGGCGTGCACTTGGCGGCGCATATCCGGGGCAGCCAGTTGCGCTTGATCCCGGGGCTGGCGCACCGTTTCCAGGAGCCGTTCAAGGCGCCGTTACTGGGGGCGGTGTTGCCTTATTTGCAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCC  >  ACB122/1212462‑1212720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: