Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 4,180,181 C→T 5.7% intergenic (+655/‑175) uvrD_1 → / → CJ019_03928 DNA helicase II/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,180,1810CT 5.7% 190.2 / 3.6 70intergenic (+655/‑175)uvrD_1/CJ019_03928DNA helicase II/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (33/33);  new base T (2/2);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01

GCCTTGGTCATTAACAGAGCTACGGTTTGTGGCTGCCCCATCGCGAAGACGCAGAATCCCTCACCGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGAAAACGCAGACATCGTCTTGACCGCTCCACCCCT  >  ACB122/4180051‑4180315
                                                                                                                                  |                                                                                                                                      
gCCTTGGTCATTAACAGAGCTACGGTTTGTGGCTGCCCCATCGCGAAGACGCAGAATCCCTCACCGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGccc                                                                                                                                    >  1:317748/1‑135 (MQ=255)
       tCATTCACACACCTACGGTTGGGGGCTCCACCATCGCGAAGCCGCATAAGCCCTCCCCGTCACTGAACAGGACTGCCGTGGATTTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGt                                                                                                                             <  6:245137/135‑1 (MQ=255)
                   cTACGGTTTGTGGCTGCCCCATCGCGAAGACGCAGAATCCCTCACCGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCa                                                                                                                 >  6:171215/1‑135 (MQ=255)
                   cTACGGTTTGTGGCTGCCCCATCGCGAAGACGCAGAATCCCTCACCGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCa                                                                                                                 >  5:343733/1‑135 (MQ=255)
                   cTACGGTGTGGGGCGGCCCCATCGCGACGACGCAGAATCCCTCACCGGCACTCAACAGGAAGGCCCTGTTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCa                                                                                                                 <  2:376652/135‑1 (MQ=255)
                    tACGGTTTGTGGCTGCCCCATCGCGAAGACGCAGAATCCCTCACCGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGTGCCCTGCCGGTTTTTACCCAGCaa                                                                                                                >  3:368925/1‑135 (MQ=255)
                    tACGGTTTGTGGCTGCCCCATCGCGAAGACGCAGAATCCCTCACCGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGTGCCCTGCCGGTTTTTACCCAGCaa                                                                                                                >  3:362757/1‑135 (MQ=255)
                    tACGGTTTGTGGCTGCCCAATCGCGAAGACGCAGAATCCCTCACCGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCTCCCTGCCGCTTTTTACCCAGCaa                                                                                                                >  3:197529/1‑135 (MQ=255)
                    tACGGTTTGTGGATGCCCCATCGCGAAGACGCAGAATCCCTCACCGTCACTGAACAGGAATGCCCTGGTATTGCTACACTACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAAt                                                                                                               >  7:86334/1‑136 (MQ=255)
                      cGGTTTGTGGCTGCCCCATCGCGAAGACGCAGAATCCCTCACCGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCa                                                                                                                 <  7:119379/132‑1 (MQ=255)
                      cGGTTTGTGGCTGCCCCATCGCGAAGACGCAGAATCCCTCACCGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCa                                                                                                                 >  8:119379/1‑132 (MQ=255)
                        gTTTGTGGCTGCCCCATCGCGAAGACGCAGAATCCCTCACCGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACg                                                                                                           <  7:56045/136‑1 (MQ=255)
                         tttGTGGCTGCCCCATCGCGACGACGCAGAAACCCTCACCGCCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACg                                                                                                           <  5:11008/135‑1 (MQ=255)
                         tttGTGGCTGCCCCATCGCGAAGACGCAGAATCCCTCACCGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACg                                                                                                           <  3:170186/135‑1 (MQ=255)
                            gtgGCTGCCCCATCGCGAAGACGCAGAATCCCTCACCGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGc                                                                                                         >  4:179457/1‑134 (MQ=255)
                                cTGCCCCATCGCGAAGACGCAGAATCCCTCCCCGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTaaa                                                                                                    >  2:9280/1‑135 (MQ=255)
                                cTGCCCCATCGCGAAGACGCAGAATCCCTCACCGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACTGCTTaaa                                                                                                    >  3:100849/1‑135 (MQ=255)
                                cTGCCCCATCGCGAAGACGCAGAATCCCTCACCGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAAc                                                                                                   >  7:120242/1‑136 (MQ=255)
                                   ccccATCGCGAAGACGCAGAATCCCTCACCGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTc                                                                                                 <  5:153904/135‑1 (MQ=255)
                                      cATCGCGAAGACGCAGAATCCCTCACCGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGCTGCTCATAGCAAGCCGCGCCCTGCCGCTTTTTACACAGCAATGACGGCTTAAACTCccac                                                                                             >  7:206008/1‑136 (MQ=255)
                                        tCGCGACCACGCAGACTCCCTCACCGTCACTGAACATGAAGCCCCTGGTATTGCTCCACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACAcc                                                                                            <  5:227321/135‑1 (MQ=255)
                                         cgcgAAGACGCTCAAGCCCTCACCGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCgg                                                                                          <  7:172241/136‑1 (MQ=255)
                                         cgcgAAGACGCAGAAACCCACACCGGCCCTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCgg                                                                                          <  7:297192/136‑1 (MQ=255)
                                          gcgaccaCGCAGAAGCCCTCCCCGTCACTGAACAGGAATGCCCTGGTATTGCTCCACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCgg                                                                                          <  2:317748/129‑1 (MQ=255)
                                           cgAAGACGCAGAATCCCTCACCGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCggg                                                                                         <  4:153194/135‑1 (MQ=255)
                                              aGACGCAGAATCCCTCACCGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCaa                                                                                      >  1:264539/1‑135 (MQ=255)
                                                aCGCAGAATCCCTCACCGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGTGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAgg                                                                                    <  4:368925/135‑1 (MQ=255)
                                                aCGCAGAATCCCTCACCGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGTGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAgg                                                                                    <  4:362757/135‑1 (MQ=255)
                                                               ccGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGc                                                                       <  6:190074/133‑1 (MQ=255)
                                                                 gTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTaaa                                                                   >  7:167230/1‑135 (MQ=255)
                                                                 gTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTaa                                                                    >  4:79566/1‑134 (MQ=255)
                                                                                tGCCATGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACACAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTc                                                    >  3:74322/1‑135 (MQ=255)
                                                                                   ccTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTc                                                 >  2:136328/1‑135 (MQ=255)
                                                                                        tATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGaa                                             >  1:179968/1‑134 (MQ=255)
                                                                                          ttGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGttttt                                                                                                                         <  3:138734/56‑1 (MQ=255)
                                                                                          ttGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGttttt                                                                                                                         >  4:138734/1‑56 (MQ=255)
                                                                                             cTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCgg                                      <  8:167230/136‑1 (MQ=255)
                                                                                              tACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCgg                                      >  3:310325/1‑135 (MQ=255)
                                                                                              tACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCgg                                      >  1:240908/1‑135 (MQ=255)
                                                                                              tACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCgg                                      <  6:104226/135‑1 (MQ=255)
                                                                                                cacGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCCCACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGc                                     >  5:138097/1‑134 (MQ=255)
                                                                                                cacGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGc                                     >  6:351730/1‑134 (MQ=255)
                                                                                                cacGACGCTGACTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCg                                    >  8:341183/1‑135 (MQ=255)
                                                                                                 acgacgCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGc                                     <  3:79566/133‑1 (MQ=255)
                                                                                                  cgacgCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACCCCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGa                                   >  6:305576/1‑134 (MQ=255)
                                                                                                   gacgCTGCCTCTAGGTGCTCATAGCAAGCCTCGCCCTGACGGTTTATACCCAGCAATGACGGATTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGaaa                                 <  5:106827/135‑1 (MQ=255)
                                                                                                    acgCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGaaaa                                <  2:264539/135‑1 (MQ=255)
                                                                                                    acgCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTAACCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACCCCGGCGaaac                                >  5:266914/1‑134 (MQ=255)
                                                                                                     cgCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGAAAAc                               <  1:136328/135‑1 (MQ=255)
                                                                                                        tGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCCGCAATGACGGCTTAAACTCCCACCgg                                                                                          >  2:376938/1‑73 (MQ=255)
                                                                                                        tGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCCCACCgg                                                                                          <  1:376938/73‑1 (MQ=255)
                                                                                                        tGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGAAAACGCa                            <  1:12708/135‑1 (MQ=255)
                                                                                                         gCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCaa                                                                                                                >  1:226558/1‑50 (MQ=255)
                                                                                                         gCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCaa                                                                                                                <  2:226558/50‑1 (MQ=255)
                                                                                                         gCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCAGGCTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTAGTCTGAAACACCGGCGAAAACGCAg                           <  5:287701/135‑1 (MQ=255)
                                                                                                          ccTCTAGGTGCTCATAGCAAGCCGCGCCCTGCGGGTTTTTACCCAGCAATGACGGCTAAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGGAAACGCAg                           <  8:86334/134‑1 (MQ=255)
                                                                                                          ccTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGAAAACGCAg                           <  8:76916/134‑1 (MQ=255)
                                                                                                          ccTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGAAAACGCAGa                          >  8:260829/1‑135 (MQ=255)
                                                                                                          ccTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGAAAACGCAGa                          >  2:349899/1‑135 (MQ=255)
                                                                                                           ctctAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGAAAACGCAGAc                         >  6:110065/1‑135 (MQ=255)
                                                                                                           ctctAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGAAAACGCAGACa                        >  8:324080/1‑136 (MQ=255)
                                                                                                                ggTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGAAAACGCAGACATCGt                    <  1:9280/135‑1 (MQ=255)
                                                                                                                 gTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGAAAACGCAGACATCGTCt                  <  8:257879/136‑1 (MQ=255)
                                                                                                                  tGCTCATAGCAAGCCGCGCACTGCAGGTTTTTACCCAGCAATGACGTCTTAAAATCACACCGGGCAAGGCATCAGAGTAAGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGAAAACGCAGACATCGTCt                  <  2:356819/135‑1 (MQ=255)
                                                                                                                   gCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGAAAACGCAGACATCGTCtt                 <  4:188445/135‑1 (MQ=255)
                                                                                                                       aTAGCAAGCCGCGCACTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGAAAACGCAGACATCGTCTTGAcc             >  6:58272/1‑135 (MQ=255)
                                                                                                                         aGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGAAAACGCa                            >  6:240363/1‑118 (MQ=255)
                                                                                                                         aGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGAAAACGCa                            <  5:240363/118‑1 (MQ=255)
                                                                                                                          gCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGAAAACGCAGACATCGTCTTGACCGCt          <  7:85425/135‑1 (MQ=255)
                                                                                                                               ccGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGAAAACGCAGACATCGTCTTGACCGCTCCAcc     <  5:110065/135‑1 (MQ=255)
                                                                                                                                cgcgCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGAAAACGCAGACATCGTCTTGACCGCTCCAccc    <  4:197529/135‑1 (MQ=255)
                                                                                                                                  cgcCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGAAAACGCAGACATCGTCTTGACCGCTCCACCCct  >  6:315894/1‑135 (MQ=255)
                                                                                                                                  |                                                                                                                                      
GCCTTGGTCATTAACAGAGCTACGGTTTGTGGCTGCCCCATCGCGAAGACGCAGAATCCCTCACCGTCACTGAACAGGAATGCCCTGGTATTGCTACACGACGCTGCCTCTAGGTGCTCATAGCAAGCCGCGCCCTGCCGGTTTTTACCCAGCAATGACGGCTTAAACTCACACCGGGCAAGGCATCAGAGTATGCTAAAAATATACGCTTTTTCGTCTGAAACACCGGCGAAAACGCAGACATCGTCTTGACCGCTCCACCCCT  >  ACB122/4180051‑4180315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: