Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 4,687,945 A→G 5.1% intergenic (‑46/‑45) CJ019_04426 ← / → yhaJ_2 hypothetical protein/HTH‑type transcriptional regulator YhaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,687,9450AG 5.1% 256.8 / 2.5 78intergenic (‑46/‑45)CJ019_04426/yhaJ_2hypothetical protein/HTH‑type transcriptional regulator YhaJ
Reads supporting (aligned to +/- strand):  ref base A (48/26);  new base G (2/2);  total (50/28)
Fisher's exact test for biased strand distribution p-value = 6.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCAGGCAGCAAGGCACTGTAGGCACCGAGGCTGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCTCGATGCCGGCTCGTTCTCCGCCGCCGCCCGCAAGCTGGGCCGTGT  >  ACB122/4687815‑4688078
                                                                                                                                  |                                                                                                                                       
cGCCAGGCAGCAAGGCACTGTAGGCACCGAGGCTGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCAGGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAAt                                                                                                                                     >  5:327786/1‑135 (MQ=255)
  ccAGGCAGCAAGGCACTGTAGGCACCGAGGCTGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAAttt                                                                                                                                   <  1:164351/135‑1 (MQ=255)
            aGGCACTGTAGGCACCGAGGCTGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATaa                                                                                                                         <  5:82572/135‑1 (MQ=255)
                aCTGTAGGCACCGAGGCTGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAga                                                                                                                     <  1:217137/135‑1 (MQ=255)
                 cTGTAGGCACCGAGGCTGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGGTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAgat                                                                                                                    <  2:60214/135‑1 (MQ=255)
                      ggCACCGAGGCTGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGatcat                                                                                                              <  8:178704/136‑1 (MQ=255)
                       gCACCGTGGCTGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGatcat                                                                                                              >  7:145177/1‑135 (MQ=255)
                       gCACCGAGGCTGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGatca                                                                                                               >  4:22288/1‑134 (MQ=255)
                       gCACCGAGGCTGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGatca                                                                                                               >  2:99307/1‑134 (MQ=255)
                       gCACCGAGGCTGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGatca                                                                                                               >  2:94088/1‑134 (MQ=255)
                       gCACCGAGGCTGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGatca                                                                                                               >  5:348214/1‑134 (MQ=255)
                        cACCGAGGCTGAGCGGGTCGCTACTGCAGTGGTTGACATGCCACGCGGTTTGTCTTTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGatcatc                                                                                                             <  5:184045/135‑1 (MQ=255)
                           cGAGGCTGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGatcatca                                                                                                            >  5:250828/1‑133 (MQ=255)
                            gAGGCTGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGatcatcatccg                                                                                                        >  8:158954/1‑134 (MQ=255)
                            gAGGCTGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGAtcatcatca                                                                                                         >  2:38590/1‑135 (MQ=255)
                            gAGGCTGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAg                                                                                                        >  7:44194/1‑136 (MQ=255)
                               gCTGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGatcat                                                                                                              >  7:68319/1‑127 (MQ=255)
                               gCTGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGatcat                                                                                                              <  8:68319/127‑1 (MQ=255)
                               gCTGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGt                                                                                                      >  2:353497/1‑135 (MQ=255)
                               gCTGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGt                                                                                                      >  3:111864/1‑135 (MQ=255)
                                cTGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGtt                                                                                                     <  1:184663/135‑1 (MQ=255)
                                 tGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTTATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTa                                                                                                   >  7:242187/1‑136 (MQ=255)
                                 tGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGttt                                                                                                    >  3:349203/1‑135 (MQ=255)
                                      gggTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGa                                                                                               <  2:372495/135‑1 (MQ=255)
                                      gggTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGa                                                                                               <  2:146119/135‑1 (MQ=255)
                                           gCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATgg                                                                                         >  7:117102/1‑136 (MQ=255)
                                                tCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCt                                                                                      >  5:391837/1‑134 (MQ=255)
                                                     tGGGTGACATGCCACGCGGTTGGTCTGTGCACGCGTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCa                                                                                >  1:163470/1‑135 (MQ=255)
                                                       cgTGACATGCCACGCGGATGGTCTGTGCACGCTTTTCTCCGTTGAGGTGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCTATAATAGATGATCATCATCAGGGTTACGGACTATGGCCTTTAACAGc                                                                              >  2:386322/2‑135 (MQ=255)
                                                        gTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCg                                                                             >  2:354972/1‑135 (MQ=255)
                                                        gTGACATGCCACGCGCTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGAAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCg                                                                             >  4:365516/1‑135 (MQ=255)
                                                          gACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGAc                                                                           >  3:366830/1‑135 (MQ=255)
                                                          gACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGAc                                                                           >  1:116256/1‑135 (MQ=255)
                                                             aTGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTccc                                                                       >  8:26676/1‑136 (MQ=255)
                                                               gCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTg                                                                     >  8:98040/1‑136 (MQ=255)
                                                                  aCGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGc                                                                   >  7:86319/1‑135 (MQ=255)
                                                                     cgGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGAt                                                               >  1:357029/1‑135 (MQ=255)
                                                                            tCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTc                                                        <  2:90374/135‑1 (MQ=255)
                                                                            tCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTc                                                        <  6:391837/135‑1 (MQ=255)
                                                                                tgCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTTATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGt                                                   <  8:242187/136‑1 (MQ=255)
                                                                                 gCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGt                                                   <  2:65256/135‑1 (MQ=255)
                                                                                   aCGCTTTTCTCCGTTGAGGGGACTTTCATGCAGGTGCATTAATGTTCATAATTTTTATCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTAAAAGAGACTCCATGGCGATTTTCCTCGCGGttc                                                 >  4:255181/1‑133 (MQ=255)
                                                                                   aCGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGc                                                 >  3:49200/1‑135 (MQ=255)
                                                                                   aCGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGc                                                 >  3:29964/1‑135 (MQ=255)
                                                                                   aCGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCt                                                >  8:370897/1‑136 (MQ=255)
                                                                                     gCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCTc                                               >  7:363898/1‑135 (MQ=255)
                                                                                      cTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCTCg                                              <  3:191383/135‑1 (MQ=255)
                                                                                             ccGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCTCGATGCCgg                                       >  2:127786/1‑135 (MQ=255)
                                                                                             ccGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCTCGATGCCgg                                       >  4:155544/1‑135 (MQ=255)
                                                                                             ccGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACAAGCGACTCCCTGGCGATTTTCCTCGCGGTGCTCGATGCCg                                        >  4:321100/1‑134 (MQ=255)
                                                                                                    ggggACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCTCGATGCCGGCTCGTtc                                >  5:81549/1‑135 (MQ=255)
                                                                                                         cTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCTCGATGCCGGCTCGTGCTccgc                          <  2:24237/135‑1 (MQ=255)
                                                                                                             ccTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCTCGATGCCGGCTCGTTCTccgccgccg                     <  8:145177/136‑1 (MQ=255)
                                                                                                             ccTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCTCGATGCCGGCTCGTTCTccgccgccg                     <  8:117102/136‑1 (MQ=255)
                                                                                                                  aGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCTCGATGCCGGCTCGTTCTCCGCCGccgccc                  >  4:201072/1‑134 (MQ=255)
                                                                                                                    gTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCTCGATGCCGGCTCGTTCTCCGCCGccgcccg                 >  6:211417/1‑133 (MQ=255)
                                                                                                                    gTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCACTGGCGATTTTCCTCGCGGTGCTCGATGCCGGCTCGTTCTCCGCCGccgcccg                 >  2:69265/1‑133 (MQ=255)
                                                                                                                     tGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCTCGATGCCGGCTCGTTCTCCGCCGCCGCCCGCaa              <  3:22288/135‑1 (MQ=255)
                                                                                                                     tGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCTCGATGCCGGCTCGTTCTCCGCCGCCGCCCGCaa              <  1:99307/135‑1 (MQ=255)
                                                                                                                      gctttAATGTACAAAATTTTTAGCAAAAAAAGATGATCATCAGCAGGTTTACTGACAAGGGCCTTTACCCGCGACTCCCTGGCGATTTTCCTCGCGGTGCTCGATGCCGGCTCGTTCTCCGCCGCCGCCCGCAAg             <  6:245106/132‑1 (MQ=255)
                                                                                                                       cATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTTCATGGCGTTGCTCGATGCGGCGCGTTCGTCccccgcccccgcc               >  8:120598/1‑131 (MQ=255)
                                                                                                                         ttAATGTTCGTAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGt                                                   >  7:298415/1‑95 (MQ=255)
                                                                                                                         ttAATGTTCGTAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGt                                                   >  7:303674/1‑95 (MQ=255)
                                                                                                                         ttAATGTTCGTAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGt                                                   <  8:303674/95‑1 (MQ=255)
                                                                                                                         ttAATGTTCGTAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGt                                                   <  8:298415/95‑1 (MQ=255)
                                                                                                                         ttAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTcc                                                          >  4:258623/1‑88 (MQ=255)
                                                                                                                         ttAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCTCGATGCCGGCTCGTTCTCCGCCGCCGCCCGCAAGCTgg         >  7:355572/1‑136 (MQ=255)
                                                                                                                         ttAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCTCGATGCCGGCTCGTTCTCCGCCGCCGCCCGCAAGCTg          >  4:284151/1‑135 (MQ=255)
                                                                                                                         ttAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCTCGATGCCGGCTCGTTCTCCGCCGCCGCCCGCAAGCTg          >  5:367417/1‑135 (MQ=255)
                                                                                                                         ttAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCTCGATGCCGGCTCGTTCTCCGCCGCCGCCCGCAAGCTg          >  4:129313/1‑135 (MQ=255)
                                                                                                                         ttAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCGCGATTCCGGCTCGTTCTACGCCGCCCCCCGCAACCTg          >  5:266449/1‑135 (MQ=255)
                                                                                                                         ttAATGTTCATAATTTTTAGCAATAAAAGATCATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTcc                                                          <  3:258623/88‑1 (MQ=255)
                                                                                                                          tAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGAGTTTCCTCGCGGTGCTCGATGCCGGCTCGTTCTCCGCCGCCGCCCGCAAGCTgg         >  6:346793/1‑135 (MQ=255)
                                                                                                                            aTGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCGCGATGCCGGCTCGTTCTCCGCCGCCGCCCGCAAGCTGGGc       <  6:24721/135‑1 (MQ=255)
                                                                                                                             tGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCTCGATGCCGGCTCGTTCTCCGCCGCCGCCCGCAAGCTGGGc       <  4:366830/134‑1 (MQ=255)
                                                                                                                             tGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCTCGATGCCGGCTCGTTCTCCGCCGCCGCCCGCAAGCTGGGc       <  4:366032/134‑1 (MQ=255)
                                                                                                                             tGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCTCGATGCCGGCTCGTTCTCCGCCGCCGCCCGCAAGCTGGGc       <  4:349203/134‑1 (MQ=255)
                                                                                                                                 cATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCCCGGTGCTCGAAGCCCGCTAGTTCTCCGCCCCCGCCCCCCAGCTGGGCCctgt  >  5:227145/1‑135 (MQ=255)
                                                                                                                                  |                                                                                                                                       
CGCCAGGCAGCAAGGCACTGTAGGCACCGAGGCTGAACGGGTCGCTACTCCAGTGGGTGACATGCCACGCGGTTGGTCTGTGCACGCTTTTCTCCGTTGAGGGGACTGTCCTGCAGGTGCATTAATGTTCATAATTTTTAGCAATAAAAGATGATCATCATCAGGTTTACTGACAATGGCCTTTACCAGCGACTCCCTGGCGATTTTCCTCGCGGTGCTCGATGCCGGCTCGTTCTCCGCCGCCGCCCGCAAGCTGGGCCGTGT  >  ACB122/4687815‑4688078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: