Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | ACB122 | 4,994,820 | G→T | 8.2% | intergenic (+322/‑62) | gabP_5 → / → CJ019_04690 | GABA permease/IS5 family transposase ISPsp10 |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB122 | 4,994,820 | 0 | G | T | 8.2% | 131.9 / 4.8 | 49 | intergenic (+322/‑62) | gabP_5/CJ019_04690 | GABA permease/IS5 family transposase ISPsp10 |
Reads supporting (aligned to +/- strand): ref base G (34/11); new base T (2/2); total (36/13) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.84e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-02 |
AGTTGTTTTCCGCAGGAGTCTGGACTCCTGCAGAAGCTGCCATCTCAAGCGACTGCGTGCTCTGGCTGTGTTAACAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCGGTACGAACTCTCGGACGTGGCCTGGGATGTGGTCTCCGATCTCTTCATCGAAA > ACB122/4994688‑4994945 | agTTGTTTTCCGCAGGAGTCTGGACTCCTGCAGAAGCTGCCATCTCAAGCGACTGCGTGCTCTGGCTGTGTTAACAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGAc > 1:3636/1‑135 (MQ=255) agTTGTTTTCCGCAGGAGTCTGGACTCCTGCAGAAGCTGCCATCTCAAGCGACTGCGTGCTCTGGCTGTGTTAACAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACg > 7:340483/1‑136 (MQ=255) ttttCCGCAGGAGTCTGGACTCCTGCAGAAGCTGCCATCTCAAGCGACTGCGTGCTCTGGCTGTGTTAACAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGcccc > 3:61841/1‑135 (MQ=255) ttttCCGCAGGAGTCTGGACTCCTGCAGAAGCTGCCATCTCAAGCGACTGCGTGCTCTGGCTGTGTTAACAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGcccc > 3:145504/1‑135 (MQ=255) gAGTCTGGACTCCTGCAGAAGCTGCCATCTCAAGCGACTGCGTGCTCTGGCTGTGTTAACAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCt > 6:49626/1‑134 (MQ=255) gAGTCTGGACTCCTGCAGAAGCTGCCATCTCAAGCGACTGCGTGCTCTGGCTGTGTTAACAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCt > 6:170351/1‑134 (MQ=255) tCTGGACTCCTGCAGAAGCTGCCATCTCAAGCGACTGCGTGCTCTGGCTGTGTTAACAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGaaa > 3:355087/1‑135 (MQ=255) ccTCCTGCAGAAGCTGCCATCTCAAGCGACTGCGTGCTCTGGCTGTGTTAACAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGCGCTGAAATTCTg > 8:390841/2‑135 (MQ=255) cTGCAGAAGCTGCCATCTCAAGCGACTGCGTGCTCTGGCTGTGTTAACAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAgg < 3:182793/135‑1 (MQ=255) cAGAAGCTGCCATCTCAAGCGACTGCGTGCTCTGGCTGTGTTAACAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCTACGCCCCTCGGGAGCTg > 1:374446/1‑120 (MQ=255) cAGAAGCTGCCATCTCAAGCGACTGCGTGCTCTGGCTGTGTTAACAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCTACGCCCCTCGGGAGCTg > 1:379208/1‑120 (MQ=255) cAGAAGCTGCCATCTCAAGCGACTGCGTGCTCTGGCTGTGTTAACAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCTACGCCCCTCGGGAGCTg < 2:374446/120‑1 (MQ=255) cAGAAGCTGCCATCTCAAGCGACTGCGTGCTCTGGCTGTGTTAACAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCTACGCCCCTCGGGAGCTg < 2:379208/120‑1 (MQ=255) cAGAAGCTGCCATCTCAAGCGACTGCGTGCTCTGGCTGTGTTAACAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTtc > 8:257138/1‑136 (MQ=255) cATCTCAAGCGACTGCGTGCTCTGGCTGTGTTAACAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCAt > 3:266763/1‑134 (MQ=255) cATCTCAAGCGACTGCGTGCTCTGGCTGTCTTAACAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCAt > 3:323949/1‑134 (MQ=255) aTCTCAAGCGACTGCGTGCTCTGGCTGTGTTAACAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCt > 2:53889/1‑135 (MQ=255) cGTGCTCTGGCTGTGTTAACAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCGACGGAAAGGAATTc > 1:88418/1‑135 (MQ=255) ctGGCTGTGTTAACAGCTCCCATCCTTGCGCCACTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGAt < 2:38143/135‑1 (MQ=255) gCTGTGTTAACAGCTCCCATCCTTGCGCCGCGCCCTAGGTGCTGTACGAAATCCCGATAAAGTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATg < 7:248881/133‑1 (MQ=255) tgtTAACAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAg > 1:241086/1‑135 (MQ=255) tAACAGCTCCCATCCTTGCTCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCg > 3:243320/1‑134 (MQ=255) tAACAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCgg > 7:67919/1‑135 (MQ=255) tAACAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCg > 5:119361/1‑134 (MQ=255) aCAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTGCGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCGGTAc > 7:348415/1‑136 (MQ=255) aCAGCTCCCATCCTTGCGCCGCGCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCGGt < 5:170351/134‑1 (MQ=255) cATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGATAGGAATTCTGTGATGGCAAAGCGGTACGAActct > 5:199759/1‑135 (MQ=255) cATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCGGTACGAActct > 1:232091/1‑135 (MQ=255) tCCTTGCGCCGCTCCCTAGGTTCTGTACTAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCGGTACGAACTCTCgg < 8:264393/136‑1 (MQ=255) tCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCGGTACGAACTCTCg > 2:219087/1‑135 (MQ=255) tCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGAAGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCGGTACGAACTCTCg > 3:303148/1‑135 (MQ=255) cTTGCGCCGATCCCTAGGTTCTGTACGAAATCACGAGAAACTGAATCGACGCCCCTCGCGAGCTGAAATTCTTGAGGGTTCTCCGCCATCTACGGAAACGAATTATGTTATGGCAAAGCGGTACGAACTCTCGGa > 2:212808/1‑135 (MQ=17) cgccgcTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCGGTACGAACTCTCGGACGt < 2:232091/134‑1 (MQ=255) ccgcTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAg < 8:211321/56‑1 (MQ=255) ccgcTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAg > 7:211321/1‑56 (MQ=255) cgcTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCGGTACGAACTCTCGGACGTGGcc < 4:61841/135‑1 (MQ=255) cgcTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCGGTACGAACTCTCGGACGTGGCCt > 8:284363/1‑136 (MQ=255) ccTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCGGTACGAACTCTCGGACGTGGCCTGGGa > 3:331496/1‑135 (MQ=255) ccTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCGGTACGAACTCTCGGACGTGGCCTGGGa > 2:165593/1‑135 (MQ=255) tACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCGGTACGAACTCTCGGACGTGGCCTGGGATGTGGTCTCCg > 5:5810/1‑135 (MQ=40) tACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCGGTACGAACTCTCGGACGTGGCCTGGGATGTGGTCTCCg > 3:79782/1‑135 (MQ=40) cGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCGGTACGACCTCTCGGACGTGGCCTGGGATGTGGTCTCCGAt > 6:321018/1‑135 (MQ=255) cGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCGGTACGAACTCTCGGACGTGGCCTGGGATGTGGTCTCCGAt > 5:92034/1‑135 (MQ=40) cGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCGGTACGAACTCTCGGACGTGGCCTGGGATGTGGTCTCCGAt > 3:185742/1‑135 (MQ=40) cGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCGGTACGAACTCTCGGACGTGGCCTGGGATGTGGTCTCCGAt > 2:311026/1‑135 (MQ=40) cGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCGGTACGAACTCTCGGACGTCGCCTGGGATGTGGTCTCCGAt > 3:372345/1‑135 (MQ=255) cGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCGGTACGAACTCTCGGACGTGGCCTGGGATGTGGTCTCCGATCTCTTCAt < 4:266763/135‑1 (MQ=40) aagtcaaTCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCGGTACGAACTCTCGGACGTGGCCTGGGATGTGGTCTCCGATCTCTTCATCGaaa < 1:24048/130‑1 (MQ=255) aagtGAAGCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCGGTACGAACTCTCGGACGTGGCCTGGGATGTGGTCTCCGATCTCTTCATCGaaa < 3:304510/132‑1 (MQ=255) | AGTTGTTTTCCGCAGGAGTCTGGACTCCTGCAGAAGCTGCCATCTCAAGCGACTGCGTGCTCTGGCTGTGTTAACAGCTCCCATCCTTGCGCCGCTCCCTAGGTTCTGTACGAAATCCCGAGAAACTGAATCGACGCCCCTCGGGAGCTGAAATTCTGTAGGGTTCTCCGCCATCTACGGAAAGGAATTCTGTGATGGCAAAGCGGTACGAACTCTCGGACGTGGCCTGGGATGTGGTCTCCGATCTCTTCATCGAAA > ACB122/4994688‑4994945 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |