Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | ACB122 | 5,381,985 | C→T | 6.2% | I509I (ATC→ATT) | CJ019_05063 → | hypothetical protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB122 | 5,381,985 | 0 | C | T | 6.2% | 211.0 / 3.2 | 64 | I509I (ATC→ATT) | CJ019_05063 | hypothetical protein |
Reads supporting (aligned to +/- strand): ref base C (34/26); new base T (2/2); total (36/28) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.65e-01 |
GTGGAGTTTGGCGGCTCGCTCAAGGCCGAGCACGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGG > ACB122/5381851‑5382119 | gTGGAGTTTGGCGTCTCGCTCAAGGCCGAGCACGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATc < 4:293120/135‑1 (MQ=255) aGTTTGGCGGCTCGCTCAAGGCCGAGCACGGCACCGGGCGCAACCTGGCGCCCTTCCTGGAGCTGGAGTGGGGGCACGACGCCGACCAGCGGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCAACCTcc > 2:207432/1‑134 (MQ=255) gcggcTCGCTCAAGGCCGAGCACGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCg < 8:8873/135‑1 (MQ=255) cggcTCGCTCAAGGCCGAGCACGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCg < 4:99221/134‑1 (MQ=255) cggcTCGCTCAAGGCCGAGCACGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGGGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCg < 5:252611/134‑1 (MQ=255) ggcTCGCTCATGGCCGAGCACGGCACCGGGCGCAACATGGCGCCCGTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGAc < 5:102216/135‑1 (MQ=255) ggcTCGCTCAAGGCCGAGCACGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGAc < 5:297416/135‑1 (MQ=255) cgctcAAGGCCGAGCACGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCGGGAGTGGGGGCACGACGCCTACCAGGTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACgtggt < 8:394637/136‑1 (MQ=255) tcAAGGCCGAGCACGGCACCGGGCGCAACATGGCGCCCGTCGTGGAGCTGGAGTGGGGGGACGCCGCCTACCCGCTGATGTGGAAGCTCAAGCGCCTGCTCGCCCCCAACGGCATCCTCCACCCCGCCggggggg > 5:244468/1‑132 (MQ=255) aaGGCCGAGCACGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGa < 8:131993/136‑1 (MQ=255) aaGGCCGAGCACGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGa < 8:138083/136‑1 (MQ=255) aaGGCCGAGCACGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGa < 7:94336/136‑1 (MQ=255) gCACGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGa < 4:2122/135‑1 (MQ=255) aCGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCCGCGGATGTGGAAGCTCCAGCGCCTGCTCGACCCCCACGGCATCCTCAACCCCGCCGGGGTGTTGAGCGAAGccc > 6:129132/1‑135 (MQ=255) aCGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGCAGCTCCAGCCCCTGCGCGACCCCAACCGCATCCTCAACCCCGCCGTGGTGTGGCGCGAAGAcc > 1:233346/1‑135 (MQ=255) aCGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATTCTCaa < 1:59305/109‑1 (MQ=255) aCGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATTCTCaa < 3:260970/109‑1 (MQ=255) aCGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATTCTCaa > 2:59305/1‑109 (MQ=255) aCGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATTCTCaa > 4:260970/1‑109 (MQ=255) cGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGatat > 4:47229/1‑135 (MQ=255) gcAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATACAcc > 5:368957/1‑135 (MQ=255) gcAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCCACCGGGTGTTGAGCGAAGACCCGGATATCCACCt > 8:3154/1‑136 (MQ=255) gcAACATGGCGCCCGTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCCACCCCCACCGGGGGTTGAGCGAAGCACCGGATATCCa > 4:87323/1‑133 (MQ=255) aTGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGCCCTGGTGTTGAGCGAAGACCCGGATATCCACCTGaa > 2:193061/1‑134 (MQ=255) gcCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACTGCATCCTCAACCCCGACCTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAAcc > 1:244576/1‑134 (MQ=255) gcCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAAcc > 3:293981/1‑134 (MQ=255) cccTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGACATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCt > 3:193966/1‑134 (MQ=255) ccTTCTTGGAGCTGGAGTCGGGCCACGACGCCTACCAGCTCATGTGGAATCTCAAGCGCCTGCTCGCCCCCAAAGCCATCCTCAACCCCGACGTGGTGTTGACCGAATCCCCCCATAACCACCTGAAAAACCTCa > 2:345250/1‑135 (MQ=255) ccTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCCCCTGAAAAACCt > 6:334537/1‑133 (MQ=255) gCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACa > 5:386175/1‑134 (MQ=255) gCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACa > 2:73162/1‑134 (MQ=255) actacgCCAACCAGCTGATGTGCAAGCTAAAGCGCCTGCTCGACCCCAACGGCAGCCTCACCCCCGACGTGGTTTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACaaaa < 4:293981/132‑1 (MQ=255) acgacgCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACaaaa > 6:367570/1‑135 (MQ=255) gacgCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAAtc < 2:8105/135‑1 (MQ=255) acgCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAAtcgt > 7:138069/1‑136 (MQ=255) ccAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACaa > 6:190059/1‑135 (MQ=255) cgcggATGTGGAAGCGCACGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGCATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGt < 1:207432/131‑1 (MQ=255) aGCTGATGTGGAAGCTCAAGCGCCTGCTCGCCCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTg > 8:30633/1‑136 (MQ=255) aTGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAAcccc < 2:357508/48‑1 (MQ=255) aTGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAAcccc > 1:357508/1‑48 (MQ=255) aTGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCAt > 4:309462/1‑134 (MQ=255) tgtgGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCAt > 4:77830/1‑133 (MQ=255) tgGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGa > 1:118878/1‑58 (MQ=255) tgGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGa < 2:118878/58‑1 (MQ=255) tgGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAg > 3:188082/1‑135 (MQ=255) tgGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAg > 3:128118/1‑135 (MQ=255) tgGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGt > 7:20197/1‑136 (MQ=255) tgGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAACTGCATCGAg > 6:331018/1‑135 (MQ=255) tCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCg < 2:302732/39‑1 (MQ=255) tCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCg > 1:302732/1‑39 (MQ=255) gcCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGc > 1:134862/1‑134 (MQ=255) tCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCg < 6:225580/114‑1 (MQ=255) tCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCg > 5:225580/1‑114 (MQ=255) tCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCgg > 4:31495/1‑135 (MQ=255) taCCCCAACTGAATCCTCAACCCGGACTTTGTGTTGAGCGAAGACCCGTATATCCAACTTAAAAACGTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGAGAACCGgtg < 2:244576/134‑1 (MQ=255) cccAACGGCAGCCTCACCCCCGACGGGGTGTGGAGCGAAGCCCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGc < 5:391611/135‑1 (MQ=255) cAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGccc > 6:322632/1‑135 (MQ=255) aaCGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGt < 8:138069/136‑1 (MQ=255) aaCGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGt < 8:62509/136‑1 (MQ=255) aCGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGt < 3:309462/135‑1 (MQ=255) aCGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGt < 3:129868/135‑1 (MQ=255) aCGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGt < 2:134862/135‑1 (MQ=255) aCGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGt < 1:6625/135‑1 (MQ=255) ggCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTc > 4:255210/1‑134 (MQ=255) tCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAggg < 7:135574/136‑1 (MQ=255) ccTCACCCCCGACGTGGTGTTGAGCGAAGACCCGGATAGCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAggg < 5:129132/135‑1 (MQ=255) | GTGGAGTTTGGCGGCTCGCTCAAGGCCGAGCACGGCACCGGGCGCAACATGGCGCCCTTCGTGGAGCTGGAGTGGGGGCACGACGCCTACCAGCTGATGTGGAAGCTCAAGCGCCTGCTCGACCCCAACGGCATCCTCAACCCCGACGTGGTGTTGAGCGAAGACCCGGATATCCACCTGAAAAACCTCAAGCCGCTGCCTGCCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGG > ACB122/5381851‑5382119 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |