Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB122 120,983 (T)9→8 intergenic (+280/+92) polA → / ← engB DNA polymerase I/putative GTP‑binding protein EngB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122120,9750T.93.0% 388.6 / 31.1 103intergenic (+272/+100)polA/engBDNA polymerase I/putative GTP‑binding protein EngB
Reads supporting (aligned to +/- strand):  ref base T (3/4);  new base . (69/24);  total (72/31)
Fisher's exact test for biased strand distribution p-value = 9.37e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

AGAGTAACTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTTCCATC  >  ACB122/120844‑121105
                                                                                                                                   |                                                                                                                                   
agagTAACTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGtttt                                                                                                                                  >  1:373617/1‑135 (MQ=255)
 gagTAACTGAATGGCTGGGGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGttt                                                                                                                                   <  4:307996/133‑1 (MQ=255)
        tGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGttttttttgccc                                                                                                                          >  3:275029/1‑131 (MQ=255)
          aaTGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCg                                                                                                                        >  5:177877/1‑134 (MQ=255)
          aaTGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCACACTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCg                                                                                                                        >  1:5516/1‑134 (MQ=255)
          aaTGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAAATTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCg                                                                                                                        >  2:404272/1‑134 (MQ=255)
               cTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGGTTTTTTTGCCCGGAAttt                                                                                                                  >  2:59914/1‑135 (MQ=255)
                           ttCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGGTTTTTTTGCCCGGAATTTGCCGGGAGCggg                                                                                                      >  1:369187/1‑135 (MQ=255)
                               aTGCGCCTATGTTGTCTTAAGTGTTGGCAGATATCTGGACCACGCCCTAGCGGTCCGGACTTGTACCCCGAAATTACCCCTCCCCATACGAAGTCCGGGGGTTTTTTTGCCAGCAATTTTCCGGGAGCgcggtgt                                                                                                  >  2:449953/1‑129 (MQ=255)
                                tGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTtc                                                                                                >  6:258764/1‑135 (MQ=255)
                                     cTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTcgc                                                                                            >  4:13497/1‑134 (MQ=255)
                                     cTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGAGGGTTTTCTcgc                                                                                            >  5:355177/1‑134 (MQ=255)
                                      tATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGa                                                                                          <  6:285358/135‑1 (MQ=255)
                                       aTGTTGTGTTAAGTGATGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGaa                                                                                         >  1:59992/1‑135 (MQ=255)
                                        tgttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        >  8:298819/1‑135 (MQ=255)
                                        tgttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        >  4:201352/1‑135 (MQ=255)
                                         gttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        <  4:278318/134‑1 (MQ=255)
                                         gttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        <  7:298819/134‑1 (MQ=255)
                                         gttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        <  3:119119/134‑1 (MQ=255)
                                         gttgtGTTAAGGGTTGGCAGATATCTGGACCCCGCCCTCGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        <  1:364071/134‑1 (MQ=255)
                                         gttgtGTTAAGGGTGGGCACATACATGGAGCCCGCCCTAGCGTTCCGGACTTTGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        <  2:230592/134‑1 (MQ=255)
                                             tgtTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGAt                                                                                   <  3:13497/135‑1 (MQ=255)
                                             tgtTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGAt                                                                                   >  2:343234/1‑135 (MQ=255)
                                                tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGCCCTTCCCCCTCCCCCTACGAAGTCCGGGGGTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTGTTCTCGCGAATGCGAtctc                                                                                >  4:159934/1‑135 (MQ=255)
                                                tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        >  5:51549/1‑127 (MQ=255)
                                                tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        <  6:51549/127‑1 (MQ=255)
                                                tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt                                                                                 >  6:242653/1‑134 (MQ=255)
                                                tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt                                                                                 >  4:14321/1‑134 (MQ=255)
                                                tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt                                                                                 >  4:262700/1‑134 (MQ=255)
                                                tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt                                                                                 >  3:88203/1‑134 (MQ=255)
                                                tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt                                                                                 >  5:120177/1‑134 (MQ=255)
                                                tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt                                                                                 >  1:193151/1‑134 (MQ=255)
                                                tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt                                                                                 >  3:475179/1‑134 (MQ=255)
                                                tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt                                                                                 >  1:385947/1‑134 (MQ=255)
                                                tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt                                                                                 >  6:526036/1‑134 (MQ=255)
                                                tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt                                                                                 >  7:407529/1‑134 (MQ=255)
                                                tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt                                                                                 >  2:392565/1‑134 (MQ=255)
                                                tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTACCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGAt                                                                                   >  7:314317/1‑132 (MQ=255)
                                                tAAGTGTTGGCAGATATCTGGACACCGCCCTAGCGGTCCGGACTTGGAAAACGAACTACCCCCTCCCCATACGAAGTCCGGGGTGTTTTTTTCCCGGAATTTGCGGGGAGCGCGTTTTCTCGCGAATGCGATGt                                                                                 >  6:488794/1‑134 (MQ=255)
                                                  aGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGa                                                                              >  2:10298/1‑135 (MQ=255)
                                                  aGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGa                                                                              >  8:78104/1‑135 (MQ=255)
                                                  aGTGTGGGTAGATATCTGGACACCGCCCTAGCGGTCCGGACTTGGACACCGAACTTCCCCCTCCCCCGACGAAGTCCGGGGTTTTTTTTGACCGGAATGTGCCGGGACCGGGTTTTGTCGCGAATGCGATGTCGa                                                                              >  6:463902/1‑135 (MQ=255)
                                                   gtgtTGGCTGATATGTGGCCCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCGCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGTAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGa                                                                              <  6:177877/134‑1 (MQ=255)
                                                     gtTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCTGGAGCGGGTTTTCTCGCGAATGCGATGTCGAAt                                                                            >  4:183435/1‑134 (MQ=255)
                                                          cAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACAccg                                                                      >  2:75110/1‑135 (MQ=255)
                                                          cAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACAccg                                                                      >  3:166331/1‑135 (MQ=255)
                                                          cAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACAccc                                                                      >  5:306321/1‑134 (MQ=255)
                                                          cAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAGGCGAGGTCGATTACAccg                                                                      >  3:342715/1‑135 (MQ=255)
                                                          cAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCAAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACAccg                                                                      >  5:154349/1‑135 (MQ=255)
                                                            gATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACAccgcc                                                                    >  3:91386/1‑135 (MQ=255)
                                                                tCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTACCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCAt                                                                <  8:157367/135‑1 (MQ=255)
                                                                 cTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        <  6:120228/110‑1 (MQ=255)
                                                                 cTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        >  5:120228/1‑110 (MQ=255)
                                                                 cTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCAtt                                                               >  5:208321/1‑135 (MQ=255)
                                                                 cTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCAt                                                                <  1:343234/134‑1 (MQ=255)
                                                                      ccccGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTcgcg                                                           <  3:421054/135‑1 (MQ=255)
                                                                          gCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTaa                                                      <  8:407529/135‑1 (MQ=255)
                                                                          gCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCAATCGCGGGTaa                                                      >  3:499400/1‑135 (MQ=255)
                                                                            ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGttt                                                                                                                                   <  3:82095/58‑1 (MQ=255)
                                                                            ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTaaaa                                                    >  6:536790/1‑134 (MQ=255)
                                                                            ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAc                                                    >  7:483373/1‑135 (MQ=255)
                                                                            ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCAATCGCGGGTaatc                                                    >  5:396021/1‑133 (MQ=255)
                                                                            ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGACCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCTCATTCGCGGGTAAAc                                                    >  6:429130/1‑135 (MQ=255)
                                                                            ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTGTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAc                                                    >  1:351337/1‑135 (MQ=255)
                                                                            ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGGTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAc                                                    >  4:215998/1‑135 (MQ=255)
                                                                            ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGGTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAc                                                    >  7:402872/1‑135 (MQ=255)
                                                                              tAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACCCCGCCGCATTCGCGGGTAAAccc                                                  >  6:279984/1‑135 (MQ=255)
                                                                                 cGGTCCGGACTGGGACCCCGAACATCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCgg                                                <  5:247020/134‑1 (MQ=255)
                                                                                    tCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCa                                            >  4:72408/1‑135 (MQ=255)
                                                                                      cGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAgg                                          >  1:482952/1‑135 (MQ=255)
                                                                                       ggACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc                                         <  3:72408/135‑1 (MQ=255)
                                                                                       ggACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc                                         <  7:502795/135‑1 (MQ=255)
                                                                                        gACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTTCCCGGAATTTGGCGTGAGCGGGTTTTCTCGCGAATGCGATGTCGCATACACCGCCGCATTCCCGGGTAGACCCGGGTCAGgc                                         >  7:228569/1‑134 (MQ=255)
                                                                                        gACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc                                         >  8:502795/1‑134 (MQ=255)
                                                                                        gACTTGCACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgcg                                        <  7:528625/135‑1 (MQ=255)
                                                                                         aCTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgcg                                        >  1:360990/1‑134 (MQ=255)
                                                                                         aCTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgcg                                        >  3:6028/1‑134 (MQ=255)
                                                                                          gtggcaCCCCTAACTGCCCCCCCCCAATACGACGTCGGGGGTTTTTTTTGCCGGTAATTGGCGGGGAGGGGGTTTTCTCGCGAATGCGATGGCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCa                                      <  5:356392/130‑1 (MQ=25)
                                                                                          cTTGGACCCCGAACTTCCCCCTCCCCTTACGAAGTCCGGGGTTTTTTTTGCCCGGATTTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCa                                      <  5:203953/135‑1 (MQ=255)
                                                                                                ccccGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGGTTTCTCGCGAAAGCGATGTCGAATACACCGCCCCCATCCCCGGTAAACCCCCGTCAGGCGCCAACCa                                 >  6:356392/1‑134 (MQ=255)
                                                                                                 cccGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACAcc                                                                       <  6:74010/95‑1 (MQ=255)
                                                                                                 cccGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACAcc                                                                       >  5:74010/1‑95 (MQ=255)
                                                                                                 cccGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCAc                                >  4:409463/1‑134 (MQ=255)
                                                                                                      aCTTCCCCCTCCCCATACTAATTACGTTTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGATTGCGATGTCGAATACACAGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCGAACCACTAAACg                          >  1:82736/1‑135 (MQ=38)
                                                                                                      aCTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAAtt                                                                                                                   >  7:166953/1‑47 (MQ=255)
                                                                                                      aCTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAAtt                                                                                                                   <  8:166953/47‑1 (MQ=255)
                                                                                                      aCTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACg                          >  4:431775/1‑135 (MQ=255)
                                                                                                      aCTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACg                          >  7:96723/1‑135 (MQ=255)
                                                                                                      aCTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCAGTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTACACg                          >  6:35803/1‑135 (MQ=255)
                                                                                                      aCTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGCGTTTTCTCGCGAATGCGATGACGAATACACCGACGCATTCGCGGGTAAACCCGGGTCAGGAGCAAACCACTAAACg                          >  8:296819/1‑135 (MQ=39)
                                                                                                           ccccGCCCCTTACTACGTCGGGGGTTTTTTTTCCCCGTAATTTGCGGGGTGCGGGTTTGCGCGCGAATGCGATGTCGAATACCCCGCCGCATTGGCGGGTAACCCGGGGGCAGGCGCAAACCACTAAACGACCg                      <  4:499400/134‑1 (MQ=255)
                                                                                                            cccacccaATACTAAGTCCGGTTTTTTTTTTGCCGTTATTTTGCGGGGCGGGGGTTTTCTCGCTATGGCTATGTCAAATACACCGCCCAATGCGCGGGTAAACCCGGGTCAGGCGCAAACCACAAAACGACCGGc                    <  8:469408/135‑1 (MQ=255)
                                                                                                            cccTCCCCATCCGATGGCGGGGGTTTTTTTGGCCCGGAATTTGCCGGGTGCGGTTTTTCTCGCGATTGCTATGTCGAATACCCCGCCGCATTCGCGGGTAAACCGGGGTCAGGCGCAAACCACTAAACGACCGGc                    <  4:166331/135‑1 (MQ=255)
                                                                                                            cccTCCCAATACTATGGCGGGGGTTTTTTTT‑‑CCCGTTAATTGGGGGGGGGGGGTTTTGCGCGCTAATGTTATGTCTAAAACCCCGCCGCATTCGCGTTTAAACCGGGGTCAGGCGAAAACCACTAAACGACCGGc                    <  8:483373/135‑1 (MQ=255)
                                                                                                            cccACCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTGGCCGGGCGCGGGTTTTCTCGCTAATGCGATGTCTAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGc                    <  3:271846/135‑1 (MQ=255)
                                                                                                                 ccaaTACGACGTCGGGGTTTTTTTTTGCCCGTAATTTGCGGGGCGGGGGTTTTCTCGCTAAGGCTATGTCTAATACACCGCCGAATTCGGGGTTAAACCCGGGTCTGGCGCAAACCACTAAACGACCGGCTTGTc               <  4:88203/132‑1 (MQ=255)
                                                                                                                               cggggTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        >  8:114049/2‑49 (MQ=255)
                                                                                                                               cggggTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        <  7:114049/48‑1 (MQ=255)
                                                                                                                                ggggTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGa                                                                                          <  1:471045/46‑1 (MQ=255)
                                                                                                                                ggggTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGa                                                                                          >  2:471045/1‑46 (MQ=255)
                                                                                                                                   |                                                                                                                                   
AGAGTAACTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTTCCATC  >  ACB122/120844‑121105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: