New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? ACB122 2460725 =101 (1.210)3 (0.040) 3/240 7.6 3.0% coding (558/831 nt) queF NADPH‑dependent 7‑cyano‑7‑deazaguaninereductase
?ACB122 3581727 = 101 (1.280)coding (517/1272 nt) ydcO_3 Inner membrane protein YdcO
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AAATCCGCTCCACACACTGCTCATGGAAGTCGGCATGCTGGCGGAAGCTGATCAGATAGGTCAGCAGGCTGGCATGGTCCAGCGCCCTGCCCTTGTACTGGACCACCACGCTGCCCCAATCCGGCTGGCCGGTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  ACB122/2460858‑2460725
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccggtgCAACCGCTGCTGGTACTGGCGATGTTCGTCACCTACGTGCTGATGCGTCGGGTGCAGCCGCGCTATGCAGTGGCTGCGGTACTGGGCGTGGGCATGCTGGTGACCGTGGCCAGCGGTACGTTGCGCAGCGAGGC  >  ACB122/3581727‑3581860
                                                                                                                                                                                                                                                                            
AAATCCGCTCCACACACTGCTCATGGAAGTCGGCATGCTGGCGGAAGCTGATCAGATAGGTCAGCAGGCTGGCATGGTCCAGCGCCCTGCCCTTGTACTGGACCACCACGCTGCCCCAATCCGGCTGGCCGGTGC                                                                                                                                       >  1:542494/1‑135
                                                                                                      CCACCACGCTGCCCCAATCCGGCTGGCCGGTGCAACCGCTGCTGGTACTGGCGATGTTCGTCACCTACGTGCTGATGCGTCGGGTGCAGCCGCGCTATGCAGTGGC                                                              >  7:286488/1‑106
                                                                                                      CCACCACGCTGCCCCAATCCGGCTGGCCGGTGCAACCGCTGCTGGTACTGGCGATGTTCGTCACCTACGTGCTGATGCGTCGGGTGCAGCCGCGCTATGCAGTGGC                                                              <  8:286488/106‑1
                                                                                                                                 CGGTGCAACCGCTGCTGGTACTGGCGATGTTCGTCACCTACGTGCTGATGCGTCGGGTGCCGCCGCGCTATGCAGTGGGTGCGGTTCTGGGCGTGGGCATGCTGGTGACCGTGGCCAGCGGTACGTTGCGCAG        >  1:154793/1‑133
                                                                                                                                 CGGTGCAACCGCTGCTGGTACTGGCGATGTTCGTCACCTACGTGCTGATGCGTCGGGTGCAGCCGCGCTATGCAGTGGCTGCGGTACTGGGCGTGGGCATGCTGGTGCCCGTGGCCAGCGGTACGTTGCGCAG        >  7:535520/1‑133
                                                                                                                                 CGGTGCAACCGCTGCTGGTACTGGCGATGTTCGTCACCTACGTGCTGATGCGTCGGGTGCAGCCGCGCTATGCAGTGGCTGCGGTACTGGGCGTGGGCATGCTGGTGACCGTGGCCCGCGGTACGTTGCGCAG        >  8:492191/1‑133
                                                                                                                                 CGGTGCAACCGCTGCTGGTACTGGCGATGTTCGTCACCTACGTGCTGATGCGTCGGGTGCAGCCGCGCTATGCAGTGGCGGCGGTACTGGGCGGGGGCATGGGGGTGACCGTGGCCAGCGGTACGTTGCGCAG        >  2:492802/1‑133
                                                                                                                                     GCAACCGCTGCTGGTACTGGCGATGTTCGTCACGTACGTGCTGATGCGTCGGGTGCAGCCGCGCTATGCAGTGGCTGCGGTACTGGGCGTGGGCATGCTGGTGACCGTGGCCAGCGGTACGTTGCGCAGCGAGGC  <  2:234528/135‑1
                                                                                                                                     GCAACCGCTGCTGGTACTGGCGATGTTCGTCACCTACGTGCTGATGCGTCGGGTGCAGCCGCGCTATGCAGTGGCTGCGGTACTGGGCGTGGGCATGCTGGTGACCGTGGCCAGCGGTACGTTGCGCAGCGAGGC  <  5:5858/135‑1
                                                                                                                                     GCAACCGCGGCTGGTACTGGCGATGGTCGTCACCTACGTGCTGATGCGGCGGGTGCAGCCGCGCTATGCAGTGGCTGCGGTACTGGGCGTGGGCATGCTGGTGACCGTGGCCAGCGGTACGTTGCGCAGCGAGGC  <  1:271110/135‑1
                                                                                                                                                                                                                                                                            
AAATCCGCTCCACACACTGCTCATGGAAGTCGGCATGCTGGCGGAAGCTGATCAGATAGGTCAGCAGGCTGGCATGGTCCAGCGCCCTGCCCTTGTACTGGACCACCACGCTGCCCCAATCCGGCTGGCCGGTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  ACB122/2460858‑2460725
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccggtgCAACCGCTGCTGGTACTGGCGATGTTCGTCACCTACGTGCTGATGCGTCGGGTGCAGCCGCGCTATGCAGTGGCTGCGGTACTGGGCGTGGGCATGCTGGTGACCGTGGCCAGCGGTACGTTGCGCAGCGAGGC  >  ACB122/3581727‑3581860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.