Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,974,0740AC57.1% 6.3 / 25.1 39Q688P (CAG→CCG) sasA_19Adaptive‑response sensory‑kinase SasA
Reads supporting (aligned to +/- strand):  ref base A (12/3);  new base C (15/5);  total (27/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.44e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGC  >  ACB122/5973940‑5974206
                                                                                                                                      |                                                                                                                                     
tGGCGCGGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCa                                                                                                                                       <  2:232679/135‑1 (MQ=255)
                     gggCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCGGGCCAGGCGCCCGCC‑TTGGCCCCGGCgcgcgcc                                                                                                                 >  7:473469/1‑129 (MQ=255)
                     gggCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCCTGGGCCCCGG‑GTGGGCAGGGCGCACggc                                                                                                                 >  3:104980/1‑133 (MQ=255)
                         cAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGGCAATCGCCCCCC‑ATGGGCAGGGCGAAAGcccccc                                                                                                             >  4:517192/1‑130 (MQ=255)
                         cAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCCTGCGCCACGC‑TTGGCCAGGGCGACCGCCCGAg                                                                                                             >  8:529941/1‑135 (MQ=255)
                                    gggCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCGCGCCCATCCCCCGGC‑GTGGCCCGGGCGCACGCCCGCGGGGGTGTTCgg                                                                                                  >  2:364615/1‑134 (MQ=255)
                                       cTTCACTCTTGACCGGGTGAGGGAGGCCGCTTATGCACGCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTAGCGCCCTGGCCATGCGCCAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCt                                                                                               <  6:135578/135‑1 (MQ=255)
                                           aCTCATGACCGGGTTTTGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCAGGCACGTATCGCCCTGGCCATGCGCCAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCg                                                                                             <  8:133649/133‑1 (MQ=255)
                                           aCTCATGACCGGGTGATGGCGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCAGGCAGGTATCGCCCTGGCCATGCGCCAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCgcc                                                                                           <  3:505272/135‑1 (MQ=255)
                                             tCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCATTCGCCCCGC‑TTGGGCCGGGGGCACGCACCAGGGGGTTTTTGGGCTCgcccg                                                                                         >  2:314787/1‑133 (MQ=255)
                                             tCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCACGCCCAGCGCCACGC‑TTGTCCCCGGCGCACCCACCCCGGGTTGTTTGACCTAgccac                                                                                         >  3:465158/1‑134 (MQ=255)
                                                  aCCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCCAGCGCCACGC‑GTTGCCACGGCGCACGCACCCCGTGGTTTTCGAGCTcgcccgccccc                                                                                    >  8:514754/1‑132 (MQ=255)
                                                   cgggggGATGGAGCCCGATTATGCACACACGCCCGTGCACCCGCGCGCCGCGCGGCATCCACGTTCCGCCCTGGCCATGCGCCTGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCt                                                                                   <  2:122283/133‑1 (MQ=255)
                                                   cggggTGTGGGCGGCCGGTTATCCACTCACGCCGGTGCACCAGCGCGCCGCGCTGCTTGCACTTTGCGCCCTGCCCTTGCGCCTGGG‑TTGGCACGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCt                                                                                   <  8:135667/133‑1 (MQ=255)
                                                    cGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGGCATTCGCCCCGCGTTGGCC‑GGGCGGACGCACGACGTGTTGTTAGAGCTcgcccgccccct                                                                                   >  8:53981/1‑130 (MQ=255)
                                                     gggTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCACGCCAAGCGCCAGGC‑GTGGCCCGGGCGAACCCCCGAGGGGTTTTTAGAGCACgccaccccccga                                                                                  >  2:347232/1‑129 (MQ=255)
                                                         gATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCACTTCGCCAGGC‑TTTTCCAGGGCGCACGCCACAGGTGTTGTTATAGCTCGCCACCCACCTAAAAcg                                                                             >  8:77164/1‑135 (MQ=255)
                                                          aTGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGGCCAGGGCCAGCGCCCGGC‑GTTGGCAGGCGGCACCCCCGACGTGTTTTTTGAGCGAGGCAGCCAGCTACAAccc                                                                            >  2:80809/1‑133 (MQ=255)
                                                                gCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGGCCAGCGCCCGGC‑GTGGGCCGGGGGCACGCCCGCGGTGTTGTTAGCGCGGGCCCGCCCGCGACAACGCGccccc                                                                      >  8:49344/1‑132 (MQ=255)
                                                                   gCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAAGCCAATAGCCCGGC‑GTTGCCAGCGCGCACGCACCACGTGGTGGTGGAGCTCGCAAGCCAGCTTAAAAGCGcccaccac                                                                   >  8:144729/1‑129 (MQ=255)
                                                                       aTGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCCTGCGCCACGC‑GTGGGCCGGGCGCCCGCACGCGGTGTTGGTGGGGCGCGCCCGCCCCCGACCACGCGCCACCCCCGGCg                                                               >  4:473663/1‑135 (MQ=255)
                                                                            cacccGCCCGGCCCCCCGCGCGCCGCGCTGCAGGCCCGTCGCCCCGGGGCCATGCCCCCGGC‑TTGGCCCGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGttt                                                          <  5:501702/131‑1 (MQ=255)
                                                                                 gCCCGTGAACCCGCGCGGCGCGCTGCAGGCTCGTTGCCCCCTGGCCATGCGCCCGGC‑TTGGCCTGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGc                                                     <  7:389207/135‑1 (MQ=255)
                                                                                  cccGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGGCCTGCGCCCCGC‑GTGGCCCCGGCGCACGCCCCCGGGGTTGTTGGGGCGCGCCCGCCCCCGACAACCCCCCCGCCCCGGCGGGGTTGCAGCg                                                    >  6:115728/1‑135 (MQ=255)
                                                                                  cccGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGGCATGCGCCCGGC‑GTGGGCCCGGCGGACGCCCGAGGGGGTGTTTGAGCTCGCCCGCCAGCGACCACCCCCCCGCCCCCGCGGGGTTGGAGCg                                                    >  4:160707/1‑135 (MQ=255)
                                                                                    cGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCGGGCCCTGGGCCCGGC‑GTGGGCCGGGCGGCCGCCCGCGGTGGTGTTGGAGGTCGCCCGCCCGCGACACCGCGCCAGCCCCCGCGGGGTTGGGGCggg                                                  >  8:515182/1‑134 (MQ=255)
                                                                                       gCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCCGGGCCTGCGCCCCGC‑GTGGGCCGGGCGGCCGCCCGAGGGGGGGTTGGCGGGCGCCCCCCCCCGTCAACCCGCCCGCCCCGGCGGGGGTGGAGCGGGTgg                                               >  2:298475/1‑134 (MQ=255)
                                                                                          ccAGCGCCCCCGGCTGCACGCAGGCAGCGCCGGGGCCTTGGGCCGGGC‑TTGGCCTGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTg                                            <  3:517192/135‑1 (MQ=255)
                                                                                            cgcgcgcCCCGCTGCATGCACGTAGCGCCCTGGCAATGCGCCCGGC‑TGGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCg                                          <  4:185323/134‑1 (MQ=255)
                                                                                              cgcgcCTCGCTGCATGCACGTATCGGCCAGGGCCTGCGCCCGGC‑GTGGGCAGGGGGGCCGCCCGAGGTGTTGTTGGGGCTCGCCCGCCCGCGTCAACGCGCCCGCCCCGGGGGGGTTGGAGGGGCTGTCTGGGag                                        >  4:127534/1‑134 (MQ=255)
                                                                                                   cTCGCTGCATGCACGTATCGCCCCGGGCCTGCGCCCGGC‑GTTGCCCGGGCGCCCGCCCGGGGTGTTGTTCGCGCGAGCCAGCCAGCTACAACGCCCCCGCCCCCGCGGGGTTGGAGCGGGTGCCGGCGCAGCGtt                                   >  8:405228/1‑135 (MQ=255)
                                                                                                    tCGGTGCATGAACGTACCGCCGGGGCCAGGCGCCCGGC‑TTGGCCCGGGGGAACGCACGAGGTGTAGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTc                                  <  2:208664/135‑1 (MQ=255)
                                                                                                             gcaCGTATCGCCCTGCCCATGCGCCAGGC‑TTGGCCAGGGCGCACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCtt                         >  7:28473/1‑135 (MQ=255)
                                                                                                                 gTACCGCCCGGGCCCCGCGCCCGGC‑TGGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCCGCAGCTTTTAc                     <  7:154262/135‑1 (MQ=255)
                                                                                                                              caggcgcCTGGC‑TGGCCCTGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGcatc        <  1:431523/132‑1 (MQ=255)
                                                                                                                                    ccAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAATCCGCATCATgcgc  >  2:121500/1‑135 (MQ=255)
                                                                                                                                    ccAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATgcgc  >  8:82368/1‑135 (MQ=255)
                                                                                                                                    ccAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATgcgc  >  7:226327/1‑135 (MQ=255)
                                                                                                                                    ccAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATgcgc  >  5:333294/1‑135 (MQ=255)
                                                                                                                                    ccAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATgcgc  >  4:65145/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                     
TGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGC  >  ACB122/5973940‑5974206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑