| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,740,399 | 0 | T | G | 52.0% | 4.2 / 13.7 | 25 | T56P (ACC→CCC) | CJ019_01643 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (5/7); new base G (0/13); total (5/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.49e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.50e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCCAGGAGCGCCTTTAGCGTGTCCACCTTCTCTTGGAAGGCCGGGTGCATTGTTGTCTGCATGGATGATCCTCGGGTGGGTCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGCGGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGTGATGATCTTCTGGTATGCCGCCTCTGCTACGA > ACB122/1740265‑1740530 | cgcCAGGAGCGCCTTTAGCGTGTCCACCTTCTCTTGGAAGGCCGGGTGCATTGTTGTCTGCATGGATGAGCCTCGGGTGGGGCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGCGGt < 4:405142/135‑1 (MQ=255) gcCAGGAGCGCCTTTAGCGTGTCCACCTTCTCTTGGAAGGCCGGGTGCATTGTTGTCTGCATGGATGATCCTCGGGTGGGTCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGCGGTg > 3:11173/1‑135 (MQ=255) gcCAGGAGCGCCTTTAGCGTGTCCACCTTCTCTTGGAAGGCCGGGTGCATTGTTGTCTGCATGGATGATCCTCGGGTGGGTCAGGCGTGGTATTCGAAGGCCGCGGCCTTACGAACGATTCGAACTTCGGCGGTg > 4:84450/1‑135 (MQ=255) gcCAGGAGCGCCTTTAGCGTGTCCACCTTCTCTTGGAAGGCCGGGTGCATTGTTGTCTGCACGGATGATCCTCGGGTGGGTCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGACCGCTTCGAACTAGTGCGGTg > 5:169482/1‑135 (MQ=37) cAGGAGCGCCTTTAGCGTGTCCACCTTCTCTTGGAAGGCCGGGTGCATTGTTGTCTGCATGGATGATCCTCGGGTGGGTCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGCGGTggg > 2:338875/1‑133 (MQ=255) cAGGAGCGCCTTTAGCGTGTCCACCTTCTCTTGGAAGGCCGGGTGCATTGTTGTCTGCATGGATGATCCTCGGGTGGGTCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGCGGTggg > 7:449249/1‑133 (MQ=255) tctTGGAAGGCCGGGTGCATTGTTGTCTGCATGGATGAGCCTCGGGGGGGTCAGGCGTGGTATTCGAAGGCCGCGGCCTTACGAACGATTCGAACTTGGGCGGTGCGCCGCGCCGGCGCGCGGCGGTCgcggc < 7:534803/133‑1 (MQ=255) gctTGGCCGGCCGGGTGCTGTGTTGTCGGCATGGTGCATCCTCGGGGGGGGCTGGCGTGGTATACCCCGCCCGCGGCCTTAGGAACGATTCTACCTTGGGCGGGGCGCCGCGCCGGCGCGCGGCGGTCgcggc < 2:106756/132‑1 (MQ=255) gAAGGCCGGGTGCATTGTTGTCTGCATGGATGATCCTCGGGTGGGTCTGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGCGGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCAt < 4:415019/132‑1 (MQ=255) aaGGCCGGGTGCATTGTTGTCTGCATGGATGATCCTCGGGTGGGGCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGCGGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATgggg < 3:276796/135‑1 (MQ=255) ttgtCTGATGGGATGAACCTGGGTGGGGTCAGGCGTGGTATTCTACGGCCTCGGCATTACTAACGATGCGAAGTTGGGCGGGGCGCCGGTCGGGGGCGGGGGGTTCGCGGCGCATGGGGTCGCTGTCGTTGATg < 8:353472/134‑1 (MQ=9) cagggATGAGCCGGGGGGGGGCCAGGCGTGGTATGCGACGGCCGCGGCCTTACGAACGATGCGACCTGGGGCGGGGCGCCGCGCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGgcatg < 3:176398/132‑1 (MQ=17) cATGGATGAGCCGCGGGTGGGGCCGGCGGGGTATTCGACGGCCGCGGCCTTACGAACGATTCGAACTTGGGCGGGGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGgcatg < 8:363122/135‑1 (MQ=17) cATGGAGGAGCCGGGGGTGGGTCTGGCGTGGTATGCGACGGCCGCGGCCTTACGAACGATGCTAACTTGGGCGGGGCGCCGCTCGGGCGCGCGGCGGGCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGgcatg < 7:282454/135‑1 (MQ=17) atgatCCGCGGGTGGGTCAGGCGTGGTATGCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGCGGGGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTg < 6:244955/135‑1 (MQ=255) ccTCGGGTGGGTCAGGCGTGTTATGCTAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGCGGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCcaca < 8:449249/135‑1 (MQ=255) ggggggggggtCTGTGGTGTTATTCTAAGGCCTGGGCATTAGTAACTTTTCGACATTGGGCGGGGCGCGGCTGGGGGGCGCGGAGGTCGCGGCGCATGGGGTCGCTGTCTTTGATGGCGGCATGCATTGCcaca < 6:443124/131‑1 (MQ=12) gggggtgtgtCTGGCGTGGACTTCGACGGCCGCGGCCTTACGAACGATTCGAACTTGGGCGGTGCGCCGCTCCGGCGCGCGGCGGGCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCcaca < 7:300537/131‑1 (MQ=18) gtCTGGGGTGGTTTTCGTTGGCCGGGGCTTAACTAACGATTCGAACTTGGGCGGGGCGCGGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCACTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCa < 2:435183/135‑1 (MQ=21) ggggTATGCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGCGGTGCGCCGCGCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAg < 4:392416/133‑1 (MQ=255) gttATGCGACGGCCGCGGCCTACCCAACGATTCGACGTGGGGCGGGGCGCCGCGCGGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGc < 4:11173/133‑1 (MQ=21) ctccacggCCGCGGCATTACTAACCACCCCACCTGGGGCGGGGCGCCGCGCCGGCGCGCGGCGGGCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCaga < 1:282735/128‑1 (MQ=25) aGGCCGCGGCCTTACTAACGATTCGAACTTGCGCGGGGCGCCGCTCCGGCGGGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAg < 6:276003/135‑1 (MQ=25) gCCTCGGCCTTAGGAAGGATGCGAACTTGGGCGGTGCGCCGGTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGtg < 6:532978/135‑1 (MQ=255) ttGGGCGGGGCGCGGCGCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGTGATGATCTTCTGGTATGCCGCCTCTGc < 8:430/135‑1 (MQ=255) cGGGGCGCGGCTCCGGCGCGCGGCGGTCGCGGCGCTTGGGGTCGCTGTCGTTGATGGAGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGTGATGATCTTCTGGTATGCCGCCTCTGCTACga < 8:334253/135‑1 (MQ=255) | CGCCAGGAGCGCCTTTAGCGTGTCCACCTTCTCTTGGAAGGCCGGGTGCATTGTTGTCTGCATGGATGATCCTCGGGTGGGTCAGGCGTGGTATTCGAAGGCCTCGGCCTTACGAACGATTCGAACTTGGGCGGTGCGCCGCTCCGGCGCGCGGCGGTCGCGGCGCATGGGGTCGCTGTCGTTGATGGCGGCATGCATTGCCACAAGACAGACCAGGGCGAGGCAGAGCGGAGTGATGATCTTCTGGTATGCCGCCTCTGCTACGA > ACB122/1740265‑1740530 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |