Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,094,8080AC50.0% 1.0 / 10.2 37L186R (CTG→CGG) CJ019_01995hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (16/2);  new base C (13/5);  total (29/8)
Fisher's exact test for biased strand distribution p-value = 4.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAGAAGC  >  ACB122/2094685‑2094940
                                                                                                                           |                                                                                                                                    
gCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCggg                                                                                                                           >  3:63924/1‑135 (MQ=255)
gCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCATCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGGCCcgcgcggccgg                                                                                                                           >  2:104037/1‑130 (MQ=255)
  cATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCATCAGTTTCTCAAGATACGTCAGCTGCTATTCTTACACCTTCGGCTCCGGCTGCTTGCGGAAAAACAGACAGCCGCGCACGACGGCGCGGCCCCCCAGGcc                                                                                                                         >  5:83290/1‑135 (MQ=255)
  cATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGcc                                                                                                                         >  2:414573/1‑135 (MQ=255)
    tGCGGGCCAGTACCGGGGCAGGGGCCGGGGCCAACAGTTTCTCAAGAAACGTCAGCTGCTCTTCAACCACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCCCGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCgg                                                                                                                       <  8:3102/135‑1 (MQ=255)
      cGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGCAGGCCACGCGCCCCGGGCCCGGc                                                                                                                     >  2:86156/1‑135 (MQ=255)
            aGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCAGCTTGCGCAAATACTGCACGCCGCGCTCGAACGCCCGCCGCGGCAGGGCCGGCGGGccg                                                                                                               >  8:63559/1‑134 (MQ=255)
            aGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCGGCTCTTCATACCCCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGCAGGCCCCGCGCGCCCCGCCCGGCgggggg                                                                                                               >  5:399690/1‑132 (MQ=255)
                      cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGCAGGCCAGGCGCGCCGGGCCGGGCGGGCCTTTTTGCAggg                                                                                                     >  4:319107/1‑135 (MQ=255)
                      cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGGCCCGCGCGCCGGGCCGGGCGGGGCGGTTTGGAggg                                                                                                     >  3:532671/1‑135 (MQ=255)
                      cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCCGGCGGGGCGGTTTGCAggg                                                                                                     >  7:280952/1‑135 (MQ=255)
                      cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTAGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAACGCCAGGCCCGCCAGGCCGCGCTGGCCGTTTTTGAggg                                                                                                     >  8:47156/1‑135 (MQ=255)
                              cGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCGTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCGGGGCTGGGCGTTTTGAAGGGCGtggtgg                                                                                             >  8:207174/1‑135 (MQ=255)
                                   cAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCGGGCTGGCCGTTTTGCCGGGGCTGTTGGGCGAg                                                                                        >  2:417169/1‑135 (MQ=255)
                                              cAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCACCCGCGCCGTGCCGGGCCGGCCGAGTTTGAGGGCCGGGTGGGCGACGGTGTTgtaga                                                                             >  3:47573/1‑135 (MQ=255)
                                                atatccGTCAGCGGCTCTTCAACCCCCTGCGGGCCCGGGTGCTCGCTCAATCACTGCCCCCCGCGCGCGCCGGCGCGGCGCGCGGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTgaaga                                                                             <  8:38489/128‑1 (MQ=255)
                                                         ccctgctATTCAACCACCTCCGGCGCCGGCGGCGGCCCCAAATCCGCCCCGCCGCGCGCGACGGCCCGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgc                                                                  <  2:402843/133‑1 (MQ=255)
                                                          gctgctCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGCGCGCCGGGCCCGGGGGGCCGTTTTTGAGGGCGGGGTGGGCGCGGGTGTTGATGAACACGatattaa                                                                 >  1:119924/1‑132 (MQ=255)
                                                            tgctCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGGCGTTTTGCAGGGCCTGTTGGGCGCGGTTGTTGAAGAACTCTATATgca                                                                 >  3:189667/1‑133 (MQ=255)
                                                              ctctTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGGGCGGGCGGGCCGGTTTGCAGGGCCGGGTGGGCGCGGGTGGTGGAGAACTCAATATtgagcag                                                             >  3:10027/1‑135 (MQ=255)
                                                               tctACAAACACCTTCGGCCCCGGCTGCTGGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgcagcag                                                             <  8:147308/134‑1 (MQ=255)
                                                                ctTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCCCGCCTGGCCGGGCTGGCCGTTTTTCAGGGGCTGGTGGCCGAGGTGTTTGTAGAACTCTATATtcagcagcc                                                           >  2:307344/1‑134 (MQ=255)
                                                                             cGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCGGGGCGGGCTGGCGGTTTTGCAGGGCCGGTTGGCCGAGGTTGTTTAAGAACTCTATATGCAGCAGCCCCACGATATGGCg                                              >  5:259113/1‑135 (MQ=255)
                                                                              ggCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGGCCGGGCGGGGCGTTTTGCAGGGCCTGGTGGCCGAGGGTGGTGAAGAACTCTATAATCAGCAGCCCCCGGATATGGc                                               >  4:289667/1‑133 (MQ=255)
                                                                              ggCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCGGGCCTTTTTGCCGGGCCGGGTGGCCGAGGTTGTTGAAGAACGAGATATGCAGCAGCCCCAGGATATGGc                                               >  8:202453/1‑133 (MQ=255)
                                                                                cTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCCGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAt                                           >  4:34250/1‑135 (MQ=255)
                                                                                       tgctTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGGCCGGGCTGGGCGGTTTGGAGGGCCGGTTGGCCGAGGGTGTTGGAGAACTCTATATGGCGCAGCACCAGGCTATGGCGGGTTTGCTTg                                    >  3:205517/1‑135 (MQ=255)
                                                                                          gtGCGAAAATACTGCACGCCGCGCGCGACGGCCCGCCGCGCCGGCCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGa                                   <  2:25605/132‑1 (MQ=255)
                                                                                                           gccgcGCTCGAAGGCCCGGCGCGCCTGGCCGGGCTGGCCGTTTTGCAGGGCCGGTTGGCCGGGGTTGTTGTAGAACTCTATATGCCGCAGCCCCAGGATATGGCGGGGTTGCTTGACCCACTGCTTGGCcgcgcc                >  3:29712/1‑133 (MQ=255)
                                                                                                           gccgcGCTCGAAGGCCAGGCGCGCCGGGCCGGGGTGGGCGTTTTTCCGGGCCTGTTGGCCGAGGTTGTTGTAGAACTCTATATTCAGCAGCACCACGATATGGGGGATTTCCTTGACCCAATGCTTgggcgcg                  >  2:314242/1‑128 (MQ=255)
                                                                                                           gccgcGCTCGAAGGCCACGCGCGCCTGGCCGGGGTGGCCGTTTTGGAGGGGCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGAc                >  8:69021/1‑135 (MQ=255)
                                                                                                            ccgcGCGCGCCGGCCCGGCGCGCGGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACa               <  3:319107/135‑1 (MQ=255)
                                                                                                                gcTCGAAGGCCCGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCGGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCgg           >  4:521763/1‑135 (MQ=255)
                                                                                                                gcTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCgg           >  4:124291/1‑135 (MQ=255)
                                                                                                                     ccggcccggcccGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag      <  6:256621/133‑1 (MQ=255)
                                                                                                                     ccggCCGGGCGCGCGGCGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag      <  3:68887/133‑1 (MQ=255)
                                                                                                                     acggCCCGGCGCGCCGGCCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag      <  2:314919/133‑1 (MQ=255)
                                                                                                                          cAGGCGCGCCTGGCCCGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTTAAGAACTCAATATGCAGCAGCACCACGAAATGGCGGATTTGCTTGACCCAAAGCTTGGCCTCGACATCGGGCAAGAAGc  >  2:165698/1‑134 (MQ=255)
                                                                                                                           |                                                                                                                                    
GCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAGAAGC  >  ACB122/2094685‑2094940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑