Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,044,6380AC51.8% 6.7 / 19.1 27Q94P (CAG→CCG) rcsC_5Sensor histidine kinase RcsC
Reads supporting (aligned to +/- strand):  ref base A (8/5);  new base C (14/0);  total (22/5)
Fisher's exact test for biased strand distribution p-value = 1.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.29e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CGTTCCAGCATTCAGTGGTGCTGGCCGAGCAGGTACGCGAACGCACCGATGCGTTGAACCAGGCCATGGCCGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCGAGACGGCCCACCAGCACCAGATTCGCCTGATTACCGACAACGTCCCGGCACTGATCGCTTACCTGAACGCCGATCTGGTCTACGAATTCACCAACAAGGTTTACGAAGAGTGGTATTGCTGGCCGCATGGCGTGATGCTGGGCCAGA  >  ACB122/3044506‑3044772
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cgTTCCAGCATTCAGTGGTGCTGGCCGAGCAGGTACGCGAACGCACCGATGCGTTGAACCAGGCCATGGCCGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCGAGACGGCcccccacc                                                                                                                                      >  4:272411/1‑133 (MQ=255)
 gTTCCAGCATTCAGTGGTGCTGGCCGAGCAGGTACGCGAACGCACCGATGCGTTGAACCAGGCCATGGCCGAGCTCAAGGCCGTCAACCGCCTGCGCAGCGAGGCCCGGCCGCGGGCCGCGCAGGCcccccaccc                                                                                                                                     >  4:49/1‑132 (MQ=255)
   tCCAGCATTCAGTGGTGCTGGCCGAGCAGGTACGCGAACGCACCGATGCGTTGAACCAGGCCATGGCCGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGGCGCGCCCGGCcaccagcccc                                                                                                                                   >  3:375258/1‑135 (MQ=255)
   tCCAGCATTCAGTGGTGCTGGCCGAGCAGGTACGCGAACGCACCGATGCGTTGAACCAGGCCATGGCCGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCGCGCCGGCCcaccagcc                                                                                                                                     >  3:513572/1‑132 (MQ=255)
   tCCAGCATTCAGTGGTGCTGGCCGAGCAGGTACGCGAACGCACCGATGCGTTGAACCAGGCCATGGCCGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCGCGCCCGCCccccagcccc                                                                                                                                   >  7:364767/1‑135 (MQ=255)
   tCCAGCATTCAGTGGTGCTGGCCGAGCAGGTACGCGAACGCACCGATGCGTTGAACCAGGCCATGGCCGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCGAGCCGGCCccccagcccc                                                                                                                                   >  6:109905/1‑135 (MQ=255)
   tCCAGCATTCAGTGGTGCTGGCCGAGCAGGTACGCGAACGCACCGATGCGTTGAACCAGGCCATGGCCGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCGAGACCGCCccccaccacc                                                                                                                                   >  2:50193/1‑135 (MQ=255)
   tCCAGCATTCAGTGGTGCTGGCCGAGCAGGTACGCGAACGCACCGATGCGTTGAACCAGGCCATGGCCGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCGACACGGCCcaccagcccc                                                                                                                                   >  3:417107/1‑135 (MQ=255)
   tCCAGCATTCAGTGGTGCTGGCCGAGCAGGTACGCGAACGCACCGATGCGTTGAACCAGGCCATGGCCGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCCAGGCGGCCccccagcacc                                                                                                                                   >  6:377482/1‑135 (MQ=255)
   tCCAGCATTCAGTGGTGCTGGCCGAGCAGGTACGCGAACGCACCGATGCGTTGAACCAGGCCATGGCCGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCCAGCCCGCCcccccgcc                                                                                                                                     >  7:182594/1‑132 (MQ=255)
             aGTGGTGCTGGCCGAGCAGGTACGCGAACGCACCGATGCGTTGAACCAGGCCATGGCCGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGTCCGGCAGCGGGCCGAGCCGGCCCACCAGCACCAGATTCGCCt                                                                                                                         >  1:36921/1‑135 (MQ=255)
             aGTGGTGCTGGCCGAGCAGGTACGCGAACGCACCGATGCGTTGAACCAGGCCATGGCCGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCGAGCCGGCCCCCCCGCCCCAGCTTCGccg                                                                                                                         >  3:488632/1‑134 (MQ=255)
                  tgCTGGCCGAGCAGGTACGCGAACGCACCGATGCGTTGAACCAGGCCATGGCCGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCGAGACGGCCCCCCCGCACCAGATTCGCCGGAATa                                                                                                                    >  1:340308/1‑135 (MQ=255)
                      ggCCGGGCTGGTAGGCGACCCCACCGTTGCGTTCAACCCGGCCTGGGCCGAGCTCAGGGCCGACACCCGCCGGCGCAGCGTGGCCCGGCCGCGGGCCGAGACGGCCCACCAGCACCAGATTCGCCTGATTACCGa                                                                                                                <  2:27214/135‑1 (MQ=255)
                       gCCGAGCAGGTACGCGAACGCACCGATGCGTTGAACCAGGCCATGGCCGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCGCGCCGGCCCCCCCGCACCAGCTTCGCCTGCTTACCgc                                                                                                                >  8:73637/1‑133 (MQ=255)
                       gCCGAGCAGGTACGCGAACGCACCGATGCGTTGAACCAGGCCATGGCCGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCGAGCCGGCCCCCCCGCCCCAGCTTCGCCGGCTTACCgc                                                                                                                >  5:239194/1‑133 (MQ=255)
                          gAGCAGGTACGCGAACGCACCGATGCGTTGAACCAGGCCATGGCCGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCGAGACGGCCCCCCCGCACCAGATTCGCCTGCTTACCGACCAc                                                                                                            >  2:84328/1‑135 (MQ=255)
                                    gcgAACGCACCGATGCGTTGAACCAGGCCATGGCCGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGGCGCGCCGGCCCACCCCCCCCATCATAGCCTGATTAACCACCACGTCCCCGccc                                                                                                  >  5:227123/1‑133 (MQ=255)
                                        aCGCACCGATGCGTTGAACCAGGCCATGGCCGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCGGGCCGGCCCCCCCGCACCAGCTTCGCCGGATTTCCGCCAACGTCCCGGCCCTgct                                                                                              >  3:338774/1‑133 (MQ=255)
                                            aCCGATGCGTTGAACCAGGCCATGGCCGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCGCGACGGCCCCCCCGCCCCACATTCGCCTGATTACCGACAACGTCCCGGCACTGCTGGCt                                                                                          >  4:117526/1‑135 (MQ=255)
                                                       gAACCAGGCCATGGCCGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCCCGCCGGCCCCCCCCCCCCACCTTCGCCAGATTACCCACCACCTCCCCGCCCCGCTAGCGGACCTGAAAGc                                                                               >  6:147154/1‑135 (MQ=255)
                                                           cAGGCCATGGCCGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCGGCGCGGCCCCCCCCCACCCGCATAGCCGGAGTTGCGCCAACCGCCCCGCACTGATCGGGTACCTGAAcccccca                                                                           >  4:7808/1‑132 (MQ=255)
                                                                 aTGGCCGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGACCGGCAGCGGGCCGAGACGGCCCACCAGCACCAGATTCGCCTGAATACCGCCAACGTCCCCGCCCTGATCGCGTACCGGAACGCCGCTCTggg                                                                      >  4:199254/1‑133 (MQ=255)
                                                                     ccGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCGAGCCGGCCCACCAGCACCAGATTAGCCGGATTTCCGACAACGTCCCGGCACGGATCGCTTACCTTAACGCCGATCTGGTCTaa                                                                  >  8:289587/1‑133 (MQ=255)
                                                                     ccGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCGAGACGGCCCCCCCGCACCAGAATCGCCGGCTTTCCGACCACGTGCCGGCCCTGCTCGCTTACCTGAACGCCGCTCGGGTCTAc                                                                  >  8:327070/1‑134 (MQ=255)
                                                                           tCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCGAGACGGGCCACCCGCACCAGATTCGCCGGATTACCGACCACGGCCCGGGACGGATCGCGTACCAGAACGGCGATCTGGTCTCCGCAttga                                                           >  3:459405/1‑133 (MQ=255)
                                                                           tCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCGAGACGGCCCACCCGCACCAGATTCGCCTGGTTACCGCCAACGTCCCGGCACTGATCGCTTACCTGCACGCCGATCTGGTCTACGAATTCa                                                           >  7:413531/1‑135 (MQ=255)
                                                                                       aCCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCGAGCCGGCCCCCCAGCACCAGATTCGCCTGATTACCGACAACGTCCCGGCCCTGATCGCTTAACTGAACGCCGATCTGGTCTACGAATTCACCAACAAGGtt                                                >  8:237314/1‑134 (MQ=255)
                                                                                       aCCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCGAGACGGCCCACCAGCCCCAGCTTCGCCTGATTACCCGCAACGTCCCGGCCCTGATCGCGTAACGGGACGCCGCTCTGGTGGACGAATTGACCAACAAgggt                                                >  1:411205/1‑132 (MQ=255)
                                                                                       aCCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCGAGACGGCCCACCAGCCCCAGATTCGCCTGATTAACGACAACGTCCCGGCACTGATCGCTTACCGTAACGCCGATCTGGTCTACGAATTCACCCACAAGGtt                                                >  2:34767/1‑134 (MQ=255)
                                                                                              gCTCAGCGAGGCCCGGCAGCGGGCCGAGCCGGCCCACCAGCACCAGATTCGCCTGATTACCGACAACGTCCCGGCACTGATCGCTTACCTGAACGCCGATCTGGTCTACGAATTCACCAACAAGGGTTACGAaga                                        >  8:152150/1‑135 (MQ=255)
                                                                                                       ggCCCGGCAGCGGGCCGCGCCGGCCCCCCCGCACCAGATTCGCCGGGTTACCGCCCACGGCCCGGCCCGGGTCGCGTTCCGGAACGCCGCTCGGGTGTACGAATTGACCAACAAGGGTTACGAAGCGGGGttttt                               >  8:235171/1‑134 (MQ=255)
                                                                                                               aGCGGGCCGAGACGGCCCCCCAGCCCCAGATTCGGCGGGTTACCGCCCACCTCCCCGCCCTGATCGGGTACCTTAACGCCGCTCGGGGCTACGCATTCACCAACAAGGTTTACGGAGAGTGGTATTGCTGGgcgc                       >  4:198515/1‑135 (MQ=255)
                                                                                                                  gggCCGCGACGGCCCACCAGCACCAGATTCGCCTGATTACCGACAACGTCCCGGCACTGATCGCTTACCTGAACGCCGATCTGGTCTACGAATTCACCAACAAGGTTTACGAAGAGTGGTATTGCTGGCCGCATg                    <  4:140353/135‑1 (MQ=255)
                                                                                                                   ggCCGCCACGGCCCACCAGCACCAGATTCGCCTGATTACCGACAACGTCCCGGCACTGATCGCTTACCTGAACGCCGATCTGGTCTACGAATTCACCAACAAGGTTTACGAAGAGTGGTATTGCTGGCCGCATgg                   <  2:23357/135‑1 (MQ=255)
                                                                                                                       gccaCGGCCCACCAGCACCAGATTCGCCTGATTACCGACAACGTCCCGGCACTGATCGCTTACCTGAACGCCGATCTGGTCTACGAATTCACCAACAAGGTTTACGAAGAGTGGTATTGCTGGCCGCATGGCGTg               <  6:12691/132‑1 (MQ=255)
                                                                                                                         caCGGCCCACCAGCACCAGATTCGCCTGATTACCGACAACGTCCCGGCACTGATCGCTTACCTGAACGCCGATCTGGTCTACGAATTCACCAACAAGGTTTACGAAGAGTGGTATTGCTGGCCGCATGGCGTGAt             <  1:291924/134‑1 (MQ=255)
                                                                                                                             gcacaccagcaccagATTCGCCTTATTACCGACAACGTCCCGGCACTGATCGCTTACCTGAACGCCGATCTGGTCTACGAATTCACCAACAAGGTTTACGAAGAGTGGTATTGCTGGCCGCATGGCGTGATGCTg         <  4:117397/132‑1 (MQ=255)
                                                                                                                               ccaccaGCACCAGATTCGCCTGATTACCGACAACGTCCCGGCACTGATCGCTTACCTGAACGCCGATCTGGTCTACGAATTCACCAACAAGGTTTACGAAGAGTGGTATTGCTGGCCGCATGGCGTGATGCTggg       <  3:209502/135‑1 (MQ=255)
                                                                                                                                caccagcaccagATTCGCCTGATTACCGACAACGTCCCGGCACTGATCGCTTACCTGAACGCCGATCTGGTCTACGAATTCACCAACAAGGTTTACGAAGAGTGGTATTGCTGGCCGCATGGCGTGATGCTGGGc      <  1:84328/135‑1 (MQ=255)
                                                                                                                                    agcaccagATTCGCCTGATTACCGACAACGTCCCGGCACTGATCGCTTACCTGAACGCCGATCTGGTCTACGAATTCACCAACAAGGTTTACGAAGAGTGGTATTGCTGGCCGCATGGCGTGATGCTGGGCCaga  <  5:109905/135‑1 (MQ=255)
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CGTTCCAGCATTCAGTGGTGCTGGCCGAGCAGGTACGCGAACGCACCGATGCGTTGAACCAGGCCATGGCCGAGCTCAAGGCCGTCAACCGCCTGCTCAGCGAGGCCCGGCAGCGGGCCGAGACGGCCCACCAGCACCAGATTCGCCTGATTACCGACAACGTCCCGGCACTGATCGCTTACCTGAACGCCGATCTGGTCTACGAATTCACCAACAAGGTTTACGAAGAGTGGTATTGCTGGCCGCATGGCGTGATGCTGGGCCAGA  >  ACB122/3044506‑3044772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑