Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,134,5670TG43.9% 16.8 / 10.8 41L143V (TTG→GTG) CJ019_02952hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (20/3);  new base G (17/1);  total (37/4)
Fisher's exact test for biased strand distribution p-value = 6.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCC‑GGCCGCGCGTTGGCCGAACAG‑CTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGC  >  ACB122/3134445‑3134699
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gCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCC‑GGCCGCGCGTTGGCCGAACCG‑c                                                                                                                          >  7:515686/1‑135 (MQ=255)
gCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCC‑GGCCGCGCGGGGGCCgccca                                                                                                                             >  7:537206/1‑129 (MQ=255)
gCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCC‑GGCCCCGCGGGGGCCggagcgc                                                                                                                           >  3:470675/1‑129 (MQ=255)
gCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCC‑CGCCGCGCGTTGGCCGAACAG‑c                                                                                                                          >  2:51890/1‑135 (MQ=255)
  gTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCC‑GGCCGCGCGTTGGCCGAACAG‑CTg                                                                                                                        >  8:168437/1‑135 (MQ=255)
  gTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCC‑GGCCGCGCGTTGGCCGAACAG‑CTg                                                                                                                        >  2:34549/1‑135 (MQ=255)
  gTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCC‑GGCCGCGCGGGGGCCGAGCaccag                                                                                                                         >  3:234349/1‑131 (MQ=255)
   tGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCC‑GGCCGCGCGTTGGGCGCACAG‑CTgg                                                                                                                       >  2:76242/1‑135 (MQ=255)
       cGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACCTCGCGGCC‑GGCCCCCCGGTTGCCGGACAG‑CGGGTggg                                                                                                                   >  3:492576/1‑133 (MQ=255)
        gACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGACCCGGCC‑GGGCCCGCCGTGGCCGCACAG‑CTggggggc                                                                                                                  >  3:238875/1‑130 (MQ=255)
            ccTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCC‑CGCCGCGCCTTGGCCGACCAG‑CAGGTGCGCGcca                                                                                                              >  2:108156/1‑135 (MQ=255)
                 cTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACACCACCACCTCGGACAACGTCGCGGCC‑GGCCCCGCGGGGGGCGGCCCG‑CTGGTGCGCGGCACcggc                                                                                                         >  3:223734/1‑135 (MQ=255)
                   cAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCC‑GGCCGCGCGGGGGCCGCCCAC‑CGGGTGCGCGCCCCccgcgg                                                                                                       >  3:106266/1‑135 (MQ=255)
                       cgcATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCC‑GGCCGCGCGGTGGGCGCACAG‑CTGGGGCGCGCCACCCGCGGCacg                                                                                                   >  7:149473/1‑133 (MQ=255)
                       cgcATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCC‑GGCCGCGCGGTGGCCGGCCAG‑CCGGGGCGCGCCCCCGGCGGCCAg                                                                                                   >  1:35479/1‑135 (MQ=255)
                           tCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCC‑GGCCGCGCGTTGGCCGAACAG‑CTGGTGCGCGACACCCGCGGCAAGGGcc                                                                                               >  2:453904/1‑135 (MQ=255)
                             cAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCC‑GGCCGCGCGTTGGCCGCACCG‑CTGGTGCGCGCCCCCGGCGGCCAGGGCCgg                                                                                             >  2:99921/1‑134 (MQ=255)
                              aGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCC‑GGCCGAGCGGTGGCCGCACCT‑CAGGTGCCCGGCACCGGCCGGAACGGccccc                                                                                            >  8:531200/1‑132 (MQ=255)
                              aGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCC‑GGCCCCGCGTTTGCCGAACCG‑CAGGTGCGCGACCCCGGCGGCACGGGCCGcc                                                                                            >  4:187080/1‑134 (MQ=255)
                                   gcagGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCC‑GGCCGCGCCTTGGCCGCCCAG‑CTGGGGCGCGGCCCCGGCGGCAAGGGCCGCATCCTg                                                                                       >  7:325747/1‑135 (MQ=255)
                                             cGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCC‑GGCCGCGCGTTGGCCGCACAG‑CTGGTGCGCGACACCGGTGGCAAGGGCCGCCTCCTGGTGTTCAACg                                                                             >  2:450329/1‑135 (MQ=255)
                                              gCTTTTCCAGATTGTTGTGCTAGCGGAGCACAGCCTAAACAACACCCCCGCTGACACCGGCGCGGCG‑GGCCGGGGGTTGGCCGAACAG‑CTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACgg                                                                            <  2:54403/135‑1 (MQ=255)
                                                  ttCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCCCCGCCGGGCCGGCGGTTGGCC‑AACAGAGGGGTGGGCACCCCCCCCGGCAAGTGCCG‑AGCCGGGTGTTTAACGGcggg                                                                        >  8:467789/1‑132 (MQ=255)
                                                    cACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCC‑GGCCCCCCGGTGGCCGCCCAG‑CTGGTGCGCGACACCCGCGGCCAAGGCCCCCACCAGGGGTTGAACgggggcga                                                                      >  5:348140/1‑129 (MQ=255)
                                                               tgCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCC‑GGCCGCGCGTTGGCCGAACAG‑CTGGTGCGCGCCACCGGCGGCCAGGGCCGCATCCTGGGGTTCAACGGCGTCTACGGGGGGCCgg                                                           >  6:87861/1‑135 (MQ=255)
                                                                                  caacaaCACCACCTCGGACAACGTCGCGGCC‑GGCCGCGCGTTTGCCGAACAG‑CTGGTGCGCGACACCGGCGGCAAGGGCCGGATCCGGGGGTTCAACGGCTTCTACGGGGGGCCGGTCTGTTCGATCCGCTacgg                                        >  8:390976/1‑134 (MQ=255)
                                                                                  caacaaCACCACCTCGGACAACGTCGCGGCC‑GGCCGCGCGGTGGCCGAACAG‑CTGGTGCGCGACACCGGCGGCAAGGGCCGCCTCCGGGGGGTGAACCGCGTCTACGGGGGGCCGGTCTGTGCGCTCCGCGacgc                                        >  1:389852/1‑134 (MQ=255)
                                                                                  caacaaCACCACCTCGGACAACGTCGCGGCC‑GGCCCCGCGTTTGCCGACCAG‑CTTGTGGGGGCCACCGGCGGCAAAGGCCGGATGCAGGTGGTGGACGTCGTCTACGGGGTGCCGGTGTGTGCGATCCGCTacca                                        >  2:331428/1‑133 (MQ=255)
                                                                                     caacaCCACCTCGGACAACGTCGCGGCC‑GGCCGCGCGGTGGCCGAACAC‑CTGGTGCGCGACACCCGCGGCAAGGGCCGCCTCCGGGGGTTGAAAGGCTTCTACGGGGTGCCGGGCTGTTCGAGACGCTAcgacga                                     >  8:11941/1‑135 (MQ=255)
                                                                                      acaaccaccTCGGACAACGTCGCGGCC‑GGCCGCGCGGTGGCCGAACAG‑CTGGTGCGCGACACCGGCGGCAAGGGCCGCCTCCTGGTGTTAAACCGCTTCTACGGGGTGCCGGTGTGTGGGATCAGCGTCGACGAg                                    >  8:359060/4‑135 (MQ=255)
                                                                                      aacaCCACCTCGGACAACGTCGCGGCC‑GGCCGCGCGGTGGCCGCACAG‑CTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCAGGTGTTGAACGGCGTGGACGGGGTGGCGGGCTGTGCGATCCGCGACGACGAg                                    >  4:301175/1‑135 (MQ=255)
                                                                                       acacCACCTCGGACAACGTCGCGGCC‑GGCCGCGCGTTGGCCGCACAG‑CTGGTGCGCGCCACCGGCGGCCAGGGCCGCCTCCGGGTGGTGAACGGCGTGTACGGGGTGGCGGGCTGTGCGCTCCGCGACGCCGAGt                                   >  1:368003/1‑135 (MQ=255)
                                                                                            accTCGGACAACGTCGCGGCC‑GGCCGCGCGTTGGCCGAACCG‑CTGGTGCGCGACACCGGCGGCAAGGGGCGCATCCTGGTGTTCAACGGCTTCTTCGGGGTTCCGGTCTGTGCGCTCCGCGACGACGAGTTgaa                               >  6:490574/1‑134 (MQ=255)
                                                                                            accTCGGACAACGTCGCGGCC‑GGCCGCGCGGTGGCCGAACCG‑CTGGGGCGCGCCACCGGCGGCCAGGGCCGCCTCCAGGGGTTCAACGGCTTCTACGGGGTGCCGGGCTGTGGGCTCCGCGACGACGAGGTgaa                               >  8:30223/1‑134 (MQ=255)
                                                                                                cGGACAACGTCGCGGCC‑GGCCGCGCGTTGGCCGCACAG‑CTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTGAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCGACGACGAGTGTaagaag                           >  7:523812/1‑134 (MQ=255)
                                                                                                          cgcgGCC‑GGCCGCGCGGTGGCCGCACAG‑CTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGCTCCGCTACGCCGAGTTGAAGAAGGTACTGGCCgc                >  4:385684/1‑134 (MQ=255)
                                                                                                            cggcc‑ggcCGCGCGTTGGCCGAACAG‑CTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGat               >  8:181568/1‑134 (MQ=255)
                                                                                                             ggcc‑ggccGCGCGTTGGCCGCACAG‑CTGGTGCGCGACACCGGCGGGAAGGGCCGCATCCGGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGatca             >  6:438016/1‑135 (MQ=255)
                                                                                                               gc‑ggccgGGGGGTGCCCCAACAG‑CTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGatcatc           <  3:80823/134‑1 (MQ=255)
                                                                                                               cgggggcgcgggTGGGCCGAACAG‑CTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGatcat            <  7:176762/129‑1 (MQ=255)
                                                                                                               cgcgcccgCGGGTTGGCCGAACAG‑CTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGatcat            <  4:167953/130‑1 (MQ=255)
                                                                                                                         cattGGCCGAACAG‑CTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGc  <  8:325747/133‑1 (MQ=255)
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GCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCC‑GGCCGCGCGTTGGCCGAACAG‑CTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGC  >  ACB122/3134445‑3134699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑