Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,927,8390TG56.0% ‑1.3 / 11.0 27Q192P (CAG→CCG) srp54Signal recognition particle 54 kDa protein
Reads supporting (aligned to +/- strand):  ref base T (10/1);  new base G (4/10);  total (16/11)
Fisher's exact test for biased strand distribution p-value = 3.71e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.00e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCAGCTGA  >  ACB122/4927707‑4927961
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aGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCggg                                                                                                                          >  7:194423/1‑135 (MQ=255)
    tGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCGGGggcg                                                                                                                      >  5:204675/1‑135 (MQ=255)
              ggtggtCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCGGGCGCGGCGCTTCGAt                                                                                                            >  8:326737/1‑135 (MQ=255)
               gtggtCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCACGCCTGGCGCGGCGCTGGGAtc                                                                                                           >  7:541625/1‑135 (MQ=255)
                     ttccccACGCCCCCCGGGCCGACCATGGCCAACCCCCCACCCTCTCCGAGCGCCTGCATTTCCGGCACTCCGAACATCCGTCCCAAGTCCCCCAGCAGCTTGCGCCCGGCCTGGCGCGGCTCTTCGATCTcggcg                                                                                                     <  4:408564/132‑1 (MQ=255)
                     tgccccATGCCCGCCGGGCCCACCATGGCGAACCCCCCACCCTCTTCGAGCGGCGCTATTTCGGCCACTTCGATCATCCGGGCCAAGTGCGCCAGCCGCCGGCGCCAGGCCGGCCGCGGCTCTTCGATCTcggcg                                                                                                     <  4:277412/132‑1 (MQ=255)
                        cccATGCACGACGGGCAGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGTCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGAGCCACTCCTGGCCCGGGTCAGCGATATaggcgg                                                                                                    >  6:134367/1‑133 (MQ=255)
                         ccATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATGATCCGTGCCAAGTGAGCCAGCCGCATGCGCCCGGCCGGGCGCGGCGCGTCGAGCTGGGCGGTCa                                                                                                 >  7:543021/1‑135 (MQ=255)
                          cATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCTGGCGCGGCGCTTCGGGCTcggcgggcgg                                                                                                >  3:399358/1‑131 (MQ=255)
                          cATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCTGGCGCGGCGCGGGGGTCTCGGCGGTcgg                                                                                                >  1:461030/1‑133 (MQ=255)
                          cATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCCGGCGCGGCGCGTGGATGTCGGCGGTCAg                                                                                                >  3:139542/1‑135 (MQ=255)
                                      gCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTGGCTCTCGGCGGTGCGGGCCAGCAGCTc                                                                                    >  6:107692/1‑135 (MQ=255)
                                            ccgggCGACCCCCCCACCCGCTGCCAGCGCCTCGATTTCCGCCACTTCGCTCACCCGTCCCAAGGCCCCCCCCCCCAGGCCCCCGCCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGc                                                                              <  2:134076/132‑1 (MQ=255)
                                                       ccccacccTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCAGGCGCGGCACTTCGAGGTAGGCGGGCCGGTGCAGCAGCTCGCGGGCGATAGGacccg                                                                   >  3:358444/1‑133 (MQ=255)
                                                        cccacccTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCGGGCGCGGCTCTTCGCACTCCGCGGTCAGGTCCAGCAGCTCGCCGGCGCTCCGCCCggc                                                                  >  4:395103/1‑134 (MQ=255)
                                                                ctTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGCGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGCTCTCGGCGGGCAGGTCCAGCCGCTCGCGGGCGCTCGGCCCGGCCCGGCCGa                                                          >  7:49592/1‑135 (MQ=255)
                                                                     aTCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCCGCTCTTCGATATCGGCGGGCCGGTCCAGCAGCGCGCGGGCGCTCGGCCCGGACCCGCCGCTgcgc                                                     >  2:69804/1‑135 (MQ=255)
                                                                         gCTCCATTGCCGCCACTGCGATCATCCGGGCCACGTCCCCCAGCAGCATGCGCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCa                                                 <  4:324533/135‑1 (MQ=255)
                                                                            cGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCGCGTCGATCTCGGCGGGCAGGTCCAGCCGCTCGCGGGCGCTCGGCCCGGCCACGCCGCTGCGcggccggc                                              >  8:411115/1‑135 (MQ=255)
                                                                                       aCTGCGACCACCCGGGCAAAGTGACCCCCCCGCAGGCCCCCGCCGGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAgg                                   <  2:527187/135‑1 (MQ=255)
                                                                                           cGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGCTCTGGGCGGTGAGGTGCAGCCGCTCGCGGGCGATCGGCCCGGGCCGGCCGAGTCGGAGCAGGCGGGGCCAAATGTTgg                               >  6:401850/1‑135 (MQ=255)
                                                                                            gATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCCGCCTGGCGCGGCTCTTGGATCTCGGCGGTCAGGGCCAGCCGCTCGCGGGCGATCGGCCCGGGCAGGCCGCTGCGCGGCCGGCGGCGCCACAGGTTGGc                              >  6:485267/1‑135 (MQ=255)
                                                                                                 tcCGTCCCAAGTGAGCCAGCAGCATGCGCCAGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCctgct                         <  4:139542/135‑1 (MQ=255)
                                                                                                      gCCACGTGCGCCCGCAGAAGGCGCACGGCGGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGc                    <  8:106016/135‑1 (MQ=255)
                                                                                                                 ccgcagcaGGCGCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTg         <  5:431785/133‑1 (MQ=255)
                                                                                                                   gcagcaTCCGCCAGGCTGGCCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTTCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCa       <  4:280190/135‑1 (MQ=255)
                                                                                                                       caggcgcCCGGCGGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCAGCTg   <  2:476076/132‑1 (MQ=255)
                                                                                                                        tggcgcCTGGCGGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCAGCTGa  <  6:136794/133‑1 (MQ=255)
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AGCTTGGCCAGGGTGGTGGTCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCAGCTGA  >  ACB122/4927707‑4927961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑