Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	5,688,637	0	A	C	49.1%	23.2 / 16.9	57	S236R (AGC→CGC)	pyrC'	Dihydroorotase‑like protein
Reads supporting (aligned to +/- strand):  ref base A (21/8);  new base C (3/25);  total (24/33)
Fisher's exact test for biased strand distribution p-value = 2.55e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGC  >  ACB122/5688506‑5688769                                                                                                                                    |                                                                                                                                     cccACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGGGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAgc                                                                                                                                    <  5:130111/134‑1 (MQ=255)    aCGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCGTCAGCCAGATCACCACCGccc                                                                                                                                >  2:328695/1‑135 (MQ=255)             ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGGGGCCTTCAGCCACATACACAGCGCCCGTGgggcgc                                                                                                                       >  7:166860/1‑135 (MQ=255)                     aGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTGCGCCAGCTGACCAGCGCCCGTGGCGCGCCGCGGATc                                                                                                               >  1:22590/1‑135 (MQ=255)                        ttCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCGTGAGCCCGCTCACCACCGCCCGTGGCGCGCGGCGGATCgcc                                                                                                            >  4:256077/1‑135 (MQ=255)                          ccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACTGAGCGGCGTGCGTGCGCCCTTCAGCCCGCTCACCACCGCCCGTGGGGCCCGGCGGATCgcccc                                                                                                          >  7:480579/1‑134 (MQ=255)                            gcgcccTGCCGGCCCCCCCGGAGCGCGCCTAACCGGGGGCCCTGCCGCGCAACCTGGGGGTGGGGGAACAGCGCGGGGGGGGGGCGCACTTCACACCTACCCCCCCGGCCGGTGGCGCGCGGCTGATCgcccagg                                                                                                        <  4:198556/131‑1 (MQ=255)                             cggccggcGGGGCATCCGGGCGCGCGCCGAACCCGGGGCCCTGGCGCGCAACCTGCGGCTGGTGGAACAGCGCGGCGGGCGTGCGCACTTCAGCCAGATCCCCCGCGCCCGGGGCGCGCGGCTGATCgcccaggc                                                                                                       <  8:4699/135‑1 (MQ=255)                                       ggCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGTGCGCACTTCCGCCAGAACACCAGCGCCCCTGGCGCGCGGCGGATCCCCCCCGCCCCCGCCTTg                                                                                             >  1:98269/1‑135 (MQ=255)                                             ccGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGGGCCCGTGAGCCCGCTCACCAGCGCCCCTGGCGCGCGGCGGCTCGCCCCGGCCCCGGCCGGGGGccgg                                                                                       >  4:88149/1‑133 (MQ=255)                                             ccGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGGGCGGGCGCCCTTCAGCCCGATAACCACCGCCCCGGGCGCGCGGCGGGTAGCCCCCGCCCCCGCCGTGGGcccg                                                                                       >  7:4699/1‑133 (MQ=255)                                                     cgcCGAACCCGGGGCCGGGGCGGGCAACCGGCTGGTGTGGTACCAGAGCGGCGGGGGGGCGCACTTCCGCCAGAGCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                               <  8:73401/135‑1 (MQ=255)                                                     cgcCGAACCCGGGGCCCTGGCGCGCACCCTGGGGGTGGTGGACCCGCGCGGCGGGCGGGCGCACTCCCCCCACAGCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                               <  3:426283/135‑1 (MQ=255)                                                     cgcCCAACCGGTGCCCCTGGCGCCCAACCTGCTGCTGGTGGACCCGCGCGGGGTGCGGGCGCACTGACGCCAGACCCCCCGCGCCGGGGCCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                               <  2:94515/135‑1 (MQ=255)                                                     cgcCCAAACGGGGCCGCTGGGGCGAAACCGGATGTTGGTGTAACTGAGCGGGGTGCGTGCGCATTACACCCAGACCACCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                               <  4:253221/135‑1 (MQ=255)                                                        cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGTGGGCCCGTCAGCCCCCTCACCCCCGCCCGGGGCGCGCGGCGGATCGCCCCCGCCCCCGCCGTGGGGCGGCCGGGAAccgc                                                                            >  4:21223/1‑135 (MQ=255)                                                        cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGGGCCCTTGCGCCCGATAACCCCCGCCCGGGGCGCGCGGCGGCTCGCCCCGGCCCCCGCCGTGGGCCTGCCGGGGAccgc                                                                            >  2:21915/1‑135 (MQ=255)                                                         gAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCACATCCGCCAGATAACCCCCCCCCGGGGCGCGCCGCTGATCGCCCACGCCCCGGCCGTGGGCCGGCCGGGTAccgcc                                                                           >  8:528873/1‑135 (MQ=255)                                                              cggggCCCGGGCGCGCAACCGGCTGCGGGGGGAACAGAGCGGCGTGCGGGCGCACTACCCCCAGACCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGt                                                                      <  8:38314/132‑1 (MQ=255)                                                               ggggCCCGGGCGCGCAACCTGCGGGTGGTGGAACCGCGCGGGGGGCGGGCGCACTTCAGCCATACCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                     <  1:361522/133‑1 (MQ=255)                                                               gTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                     <  3:149288/135‑1 (MQ=255)                                                               gTGGCCCTGGCGCGCAACCTGCTGCTGGGGGAACAGCGCGGCGGGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                     <  3:523664/135‑1 (MQ=255)                                                               gTGGCCCTGGCGCGCAACCTGCTGCTGGGGCACCAGCGCGGCGGGCGTGCGCACTTCCGCCAGATCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                     <  5:127227/135‑1 (MQ=255)                                                               gTGCCCCTGGCGCGCAACCTGCGGCTGGGGGACCAGAGCGGGGTGCGGGCGCACTTCAGCCACATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                     <  5:226232/135‑1 (MQ=255)                                                                gggCCCTGGCGCGCAACCTGCTGCTGGTGCAACAGCGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAg                                                                    <  7:23491/134‑1 (MQ=255)                                                                   ccggggCGCGCACCCTGCTGGGGGGGCAACAGCGGGGGGTGCGGGCGCACTCCCGCCAGACCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAgcgc                                                                 <  1:328695/131‑1 (MQ=255)                                                                    ccTGGCGCGCAACCTGCTGCGGGTGGAACAGCGCGGCGTGCGTGCGCACTTCAGCCAGACCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCt                                                                <  4:223903/135‑1 (MQ=255)                                                                     gtGGCGCGCACCCTGCGGGTGGGGGAACAGAGGGGGGTGCGGGCGAACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTg                                                               <  8:166860/134‑1 (MQ=255)                                                                                ccggctgctgGTGGACCAGCGCGGCGGGGGTGCGCACTCCAGCCAGATCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGAt                                                    <  3:372389/132‑1 (MQ=255)                                                                                             gAACAGAGCGGCGTGCGTGCGCACGTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCGGATCGCCCCGGCCCCGGCCGTGGGCCGGCCGGGAACCGCCGCTGTAGCCCGGGACCAGCTGATCCTCCCCGACGAg                                       >  8:535969/1‑135 (MQ=255)                                                                                                   cgcggcgGGCGGGCGCACTGCCGCCAGAACCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTg                                 <  8:37183/134‑1 (MQ=255)                                                                                                     cggcggGCGTGCGCACTTCAGCCCGACCCCCCCCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                               <  7:48804/135‑1 (MQ=255)                                                                                                     cggcggGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                               <  3:415852/135‑1 (MQ=255)                                                                                                     cggcgTGCGCGCGCACTTCCGCCACACCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                               <  8:145258/135‑1 (MQ=255)                                                                                                      ggctTGCGGGCCAACTCCAGCCACTTCACCCCCGCCGGTGGCGCGAGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGGCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcgc                              <  6:108901/135‑1 (MQ=255)                                                                                                      ggcgTGCGTGCGCACTTAAGCCCGATAACCACCGCCCGTGGCGCGCGGCTGATCGCCCCGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCGGTACCAGCGGGTACTCCCCGGCGCGTCGcggggc                              >  8:85012/1‑130 (MQ=255)                                                                                                          tgcgggcgAACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                          <  3:314556/135‑1 (MQ=255)                                                                                                          ggggggcgcACTGCCGCCACACCCCCCGGGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                          <  3:256077/130‑1 (MQ=255)                                                                                                          ggcgtgcgAACTTCCGCCCGAGCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                          <  3:110235/134‑1 (MQ=255)                                                                                                          ggcgggcgCACTTCCGCCAGACCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                          <  5:228977/134‑1 (MQ=255)                                                                                                          ggcgggcgCACTTCCCCCACACCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                          <  1:90783/134‑1 (MQ=255)                                                                                                          ggcgggcgCACTGCAGCCAGAACACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                          <  2:355191/134‑1 (MQ=255)                                                                                                           gcgggcgCACTTCACCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGtt                         <  4:387192/135‑1 (MQ=255)                                                                                                            cgtgcgCACCTCCCCCAGATCACCCGCCCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTtc                        <  8:222781/135‑1 (MQ=255)                                                                                                               gcgcACTTCAGCCAGATCACCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc                     <  6:266978/135‑1 (MQ=255)                                                                                                                     ttCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCt                >  6:264496/1‑134 (MQ=255)                                                                                                                     ttCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCt                >  8:253814/1‑134 (MQ=255)                                                                                                                         gCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTAcc           >  1:38047/1‑135 (MQ=255)                                                                                                                          ccAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCa          >  1:421151/1‑135 (MQ=255)                                                                                                                             gATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGt       >  4:64449/1‑135 (MQ=255)                                                                                                                                ccccAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                          <  5:403068/111‑1 (MQ=255)                                                                                                                                cACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                          >  6:403068/1‑113 (MQ=255)                                                                                                                                cACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCa    >  8:218903/1‑135 (MQ=255)                                                                                                                                  ccAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCTGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAgc  >  8:500190/1‑135 (MQ=255)                                                                                                                                  ccAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAgc  >  2:75827/1‑135 (MQ=255)                                                                                                                                  ccAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAgc  >  1:155790/1‑135 (MQ=255)                                                                                                                                  ccAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGGGCTCCCGCCGGTACCACGTGCAgc  >  4:535329/1‑135 (MQ=255)                                                                                                                                    |                                                                                                                                     CCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGC  >  ACB122/5688506‑5688769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑