Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,835,8770TG53.8% 1.1 / 17.7 28E658A (GAG→GCG) barASignal transduction histidine‑protein kinaseBarA
Reads supporting (aligned to +/- strand):  ref base T (3/9);  new base G (0/14);  total (4/24)
Fisher's exact test for biased strand distribution p-value = 8.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.92e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGG  >  ACB122/1835747‑1836010
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aTAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGGCGGTGGGGTTGGTCCCCACCGccc                                                                                                                                 >  5:1059/1‑136 (MQ=255)
                 ccAGTACCTCGGCACCCAGGTCTTCGAGCAGGTTTTTTACCAGCAGCAGGTTGGCGGCTTTGTCGTCGACGCAGAGTATTTTCGGTCGGCGCTGGCCGTTGGGGTGGCCCCCCTCGGCCAGCGGACGGCGAGGTTGc                                                                                                                <  7:266554/137‑1 (MQ=255)
                                       tgcGAGCCGGGTTTTACCCAGCAGCAGGTGGGCGCCATGGGCGTCCCCGCAGTGTATTTGCGGGCGGCGCGGGCCGGTGGGGGGCCCCCCCGCGCCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGcgcc                                                                                          <  2:204359/135‑1 (MQ=255)
                                                      aaccaccaccTGGTGGGCGGATTTGGCGGCGACGCAGTGTTTTTTCGGGCGGCGGTGGCGTTGGGGGTGGCGCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGc                                                                           <  8:19427/136‑1 (MQ=255)
                                                           cccgcagGTGGGCGGCTTTGGCGTCGCCGCTGTTTTTTTTGGGGCGGGGCGGGCGTTGGGGGTGGCCCCCCCCGGCCCGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTc                                                                      <  4:110537/134‑1 (MQ=255)
                                                            cagccgGTGGGCGGCATTGGCGTCGACGCAGCTTATTTTCGGCCGGCGGTGGCCGTTGGGGTGCCCCCCCGCGGCCAGGGGACGGCGAGGGTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCg                                                                     <  6:336253/137‑1 (MQ=255)
                                                                              gtggtcgACGCAGTTTATTTGGGGGGGTCGGTGGCGGTTGGGGTGGCCCCCCGCGGCCTGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGc                                                   <  3:252678/134‑1 (MQ=255)
                                                                              gtcgtcGACGCAGAGTATTTTCGGGCGGCGCGGGCCGTTGGGGTGGCGCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGc                                                   <  2:264112/137‑1 (MQ=255)
                                                                                   cgccGCTGAGTTTTTTCGGGCGGGGGTGGCGGTTGGGGTGGCCCCCCGCGGCCCGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATgg                                              <  4:102901/134‑1 (MQ=255)
                                                                                        cAGAGTTTTTTCGGGCGTCGCTTTCCGTTGGGGGTGCCCCCCGCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGtt                                         <  6:1059/137‑1 (MQ=255)
                                                                                             aaTTTTCGGGCGGCGCTGGCCGTGGGGGTTGACCCCCTCGGCCAGCGGACGGCGAGGTTGCACCATCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCa                                    <  8:100705/136‑1 (MQ=255)
                                                                                                  tCGGTCGTCGCTGACCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTAc                               <  8:120756/137‑1 (MQ=255)
                                                                                                  ggggTCGGCGCGGGCGGTGGGGGTGCCCCCCCGGGCCCCGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTAc                               <  3:179015/135‑1 (MQ=255)
                                                                                                  gcGGTGGGCGCGGGCCGTTGGGGTGGCCCCCCGCGGCCGGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGCGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTat                               <  8:115893/136‑2 (MQ=255)
                                                                                                  gcGGGCGGCGCGGGCCGTTGGGGTGGCCCCCCTCCGCCAGCGCACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTAc                               <  6:279082/136‑1 (MQ=255)
                                                                                                                     ggggGTGCCCCCGGCGCCCCGCGGACGGCGAGGGTGCACCAGCTCCAGCAAGAGGCGCCGCAGCGTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCt            <  8:179407/137‑1 (MQ=255)
                                                                                                                          ggccccccTCGGCCCGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCggtg       <  4:112193/133‑1 (MQ=255)
                                                                                                                          gcccccccccGCCCTGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCggtg       <  1:80329/133‑1 (MQ=255)
                                                                                                                            cTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCggtggt     >  5:273261/1‑137 (MQ=255)
                                                                                                                             cccccTGGGCCTGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTc    <  7:217375/136‑1 (MQ=255)
                                                                                                                              ccccTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTg                                                                <  8:283501/76‑1 (MQ=255)
                                                                                                                              ccccTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTg                                                                >  7:283501/1‑76 (MQ=255)
                                                                                                                              ccccTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCg   >  3:199082/1‑137 (MQ=255)
                                                                                                                               cccGCGGCCTGGGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCgg  <  1:33240/137‑1 (MQ=255)
                                                                                                                               cccGCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCgg  <  5:334463/137‑1 (MQ=255)
                                                                                                                               cccGCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCgg  <  4:135424/137‑1 (MQ=255)
                                                                                                                               cccGCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCgg  <  2:28865/137‑1 (MQ=255)
                                                                                                                               cccGCGCCCCGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCgg  <  7:39216/137‑1 (MQ=255)
                                                                                                                               cccGCGCCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCgg  <  1:27007/137‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                     
ATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGGTTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCTCCCCTCGGCCAGCGGACGGCGAGGTTGCACCAGCTCCAGCAAGAGGCGCCGCAGCTTGCGGGTGCAGGTCGGCTTGGACAGCAACTGGCCATGGCCGTTGGGCAGGTACGGGTGGTACAGCGCCTGCTCGGTGGTCGG  >  ACB122/1835747‑1836010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑