Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,308,3210CG35.4% 68.5 / 15.3 48A230P (GCC→CCC) fucAL‑fuculose phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (5/26);  new base G (17/0);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 6.20e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGGCACCTTTGCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATCA  >  ACB122/2308186‑2308449
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cggCACCTGTGCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGcc                                                                                                                                  <  2:286429/136‑1 (MQ=255)
aGGCACCTTTGCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGcc                                                                                                                                  <  5:374746/137‑1 (MQ=255)
     ccTTTGCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑ttt                                                                                                                            <  6:181027/137‑1 (MQ=255)
     ccTTTGCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑ttt                                                                                                                            <  6:1862/137‑1 (MQ=255)
       tttGCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGc                                                                                                                          <  6:205680/137‑1 (MQ=255)
       tttGCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGc                                                                                                                          <  5:346641/137‑1 (MQ=255)
           ccGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCGC‑TTTGCCCac                                                                                                                      >  6:58267/1‑136 (MQ=255)
             gttgttGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTg                                                                                                                    <  4:48737/137‑1 (MQ=255)
                   gAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCgg                                                                                                              <  6:244301/137‑1 (MQ=255)
                              aCAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGG‑GTTGACCCGGTCTTCTGGGATGgggggg                                                                                                   >  4:27198/1‑137 (MQ=255)
                              aCAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGC‑GTTGTCCAGTGCTTGGGGGATTgggggg                                                                                                   >  5:231939/1‑137 (MQ=255)
                              aCAGCTTCGATCAGCCCAGGCAGTCGCCATCGCCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTTGCCACTGCTACGGGGATTGGGCgg                                                                                                   >  8:155929/1‑137 (MQ=255)
                                 gCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGC‑GTTTCCCAGTGCTTGGGGGGTGggggggttt                                                                                                >  3:81453/1‑134 (MQ=255)
                                   ttCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTc                                                                                              <  7:305337/137‑1 (MQ=255)
                                     cGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCTC‑TTTGGCCCGTGCGTCGGGGGTGGGGGGGGTGTCAc                                                                                            >  5:62138/1‑137 (MQ=255)
                                       aTCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGC‑GTTGCGCAGTGGGTCGGGGATGGGGGGGGTGTGAccc                                                                                          >  5:56321/1‑136 (MQ=255)
                                        tCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCTC‑TTTGGCCAGTGCTTGGGGGTTGGGGGGGGTGTCACCgg                                                                                         >  3:285837/1‑137 (MQ=255)
                                        tCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCTC‑TTTGCCCCGGGGTTGGGGGTTGGGGGGGGTGTGACCgg                                                                                         >  5:67251/1‑137 (MQ=255)
                                        tCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCTC‑TTTGCCCAGTGCGTCGGGGTTGGGGGGGGTGTCACCgg                                                                                         >  6:189413/1‑137 (MQ=255)
                                        tCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGC‑TTTGGGCAGTGGGTGGGGGTTGGGGCGGGTGGGACCgg                                                                                         >  7:250206/1‑137 (MQ=255)
                                               aGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCTC‑TTTGGCCAGTGGTTAGGGGTTTGGGCGGCTGTGACCGGCGCCCaa                                                                                  >  7:162624/1‑136 (MQ=255)
                                                  cAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGc                                                                               <  1:112151/137‑1 (MQ=255)
                                                      cGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGTGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCcagc                                                                           <  2:173500/137‑1 (MQ=255)
                                                        ccATGTGCACGCAAGGCACTGCGCTCGTAGTATCTGAAGGCAGCACCGTGACGTTTGGGTGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCcagcag                                                                         <  5:210/137‑1 (MQ=255)
                                                        ccATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGGGC‑TTTGGCCAGTGGGTGGGGGGTGGGGGGGATGTGACCGGCGCCCATGGCccgcag                                                                         >  7:104330/1‑137 (MQ=255)
                                                        ccATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGC‑TTTGCCCAGTGGGTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCcaccag                                                                         >  2:229569/1‑137 (MQ=255)
                                                        ccATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCcagcag                                                                         <  2:159471/137‑1 (MQ=255)
                                                         cATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGGGC‑TGTGGCCAGTGGGTTGGGGGTTGGGGGGCTGTGGACGGCGCCCATGGCcacccgt                                                                        >  3:331259/1‑132 (MQ=255)
                                                         cATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGC‑GTTGGCCCGTGGGTCGGGGCTGGGGGGGGTGTGACCGGCGCCCATGGGCCGCAGt                                                                        >  5:219077/1‑137 (MQ=255)
                                                         cATGGGCACCCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGC‑CATTACCACGGCGACCGGGAGGCGGCGGGTATCACCCGCGCCCCTGGCccgcaga                                                                        >  6:13048/1‑136 (MQ=255)
                                                          aTGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCTG‑TTTGGCCCGTTCTTAGGGGATAGGGGGGTTGTAACCCGCGCCCATGGCcagaaatt                                                                       >  6:210/1‑132 (MQ=255)
                                                                   cacggCACCGCGCGCGTACTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGTGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTTCATCTTtg                                                              <  8:157377/134‑1 (MQ=255)
                                                                        cACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTgcg                                                         <  2:265425/137‑1 (MQ=255)
                                                                              gcgcgTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGCGTTTGCCC‑GTGGGGGGGGGGTGGGGGGGCTGTGACCGGCGCCCCAGGCCCGCCGGTGGCTCTTTTGTgcgcgcggg                                                   >  1:107090/1‑134 (MQ=255)
                                                                                gcgTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGaa                                                 <  3:27198/137‑1 (MQ=255)
                                                                                       agCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGGCGTGTGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCaga                                          <  3:114000/137‑1 (MQ=255)
                                                                                             aGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAgagcgagc                                    <  5:189413/137‑1 (MQ=255)
                                                                                                cagcaCCGTGACGTTTGGGGGTGGAAATCCAGTCGGGGTCCTC‑TTGGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACg                                 <  8:104771/137‑1 (MQ=255)
                                                                                                           cGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTTACCAGTGGTTCGGGGATGGGGCGGATGTCACCGGCGCCCAAGGCCAGCAGTTGCATCTTTTCTTCGCGCTCGAACAGCAGAGCGCGCACCCAGGCGTACtc                      >  6:166854/1‑137 (MQ=255)
                                                                                                           cGTTTGGGGGTGGAAATCCAGTCGTGGGCCGC‑TTTTGCCAGTGCGTCGGGGGTTGGGGGGATTTGACCCGCGCCCAAGGCCAGCAGTTGCATATTTTCTTCGCGCGCGAACACCAGACCGAGCCCGCCCGCTTccac                      >  4:191691/1‑135 (MQ=255)
                                                                                                              ttGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCtcgat                   <  6:275619/137‑1 (MQ=255)
                                                                                                                   ggTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAAc              <  4:199816/137‑1 (MQ=255)
                                                                                                                   ggTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAAc              <  5:162778/137‑1 (MQ=255)
                                                                                                                   ggTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAAc              <  1:159477/137‑1 (MQ=255)
                                                                                                                   ggTGGAAATCCAGTCGTGGGCCGC‑TTTTGCCAGTGGTTAGGGGATTGGGGGGATGTCACCCGCGCCCCAGGCCAGCAGTTGGAACTTTTATGCGCGCGCGCAAACCAGAGCGAGCACCCAGGCTTACTCGATAGaaa              >  4:170965/1‑136 (MQ=255)
                                                                                                                       taaaTCCAGGCGTGGGCCTC‑TTTGGCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTGCTCGATCGAACGccc          <  7:81073/136‑1 (MQ=255)
                                                                                                                        aaaTGCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCa         <  1:106156/137‑1 (MQ=255)
                                                                                                                             cAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGAt    <  3:191691/137‑1 (MQ=255)
                                                                                                                              aGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCATCAGTTGGATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCAGAGCGATc   <  5:352530/137‑1 (MQ=255)
                                                                                                                               gTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATca  <  5:58267/137‑1 (MQ=255)
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AGGCACCTTTGCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATCA  >  ACB122/2308186‑2308449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑