Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,641,8430AC30.9% 87.5 / 11.0 55Q36P (CAG→CCG) CJ019_02499hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (12/26);  new base C (17/0);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 8.06e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTGCGCCCCCCGTTTCGAGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCTGTGCCGTGCCATCCTCG  >  ACB122/2641710‑2641974
                                                                                                                                     |                                                                                                                                   
ttGCGCCCCCCGTTTCGAGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGcg                                                                                                                                  >  6:370986/1‑136 (MQ=255)
ttGCGCCCCCCGTTTCGAGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGca                                                                                                                                  >  6:336643/1‑136 (MQ=255)
ttGCGCCCCCCGTTTCGAGTGCTCCAAATGCCGCGCCCTGCCTGGTCACTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCt                                                                                                                                  >  6:188692/1‑137 (MQ=255)
               cGAGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGcc                                                                                                                   <  2:151477/137‑1 (MQ=255)
               cGAGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCGGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGcc                                                                                                                   <  6:263452/137‑1 (MQ=255)
                    gCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCg                                                                                                              <  5:62151/137‑1 (MQ=255)
                    gCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCAAGGAAAGCCGCGTCCACCTCGTGGCGCCCCCACTCGAGCGTGGCGGGcgcgggg                                                                                                              >  4:8580/1‑131 (MQ=255)
                         aaaTGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGt                                                                                                         <  4:112236/137‑1 (MQ=255)
                                 cgcCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTtgtg                                                                                                 <  4:122493/137‑1 (MQ=255)
                                  gcCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCCCGCCACCGGGGCGGGCCGGGGGCCCGGACATtgtgt                                                                                                >  3:56451/1‑137 (MQ=255)
                                           ggTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCGCGCGCTGGGCGTGCCGGGCGCCCCGACTTTGGGGTGCGGccgg                                                                                       >  6:225458/1‑135 (MQ=255)
                                                cTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGcc                                                                                  >  6:164585/1‑137 (MQ=255)
                                                cTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCCCCCGCGCGAGCTGGGGGGGCCGGGCGCCCGGACATTGTGTGGCGGGCTGCTGcc                                                                                  >  8:214474/1‑137 (MQ=255)
                                                   tATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTc                                                                                                                                 >  1:201249/1‑87 (MQ=255)
                                                   tATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTc                                                                                                                                 <  2:201249/87‑1 (MQ=255)
                                                         tATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGcc                                                                         <  2:244740/137‑1 (MQ=255)
                                                          aTCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCGGGAGGAGGGGGTGCCGGGCGACCGTGCTTTGTGTTGGGGCCTGGGGCCACCACAGccc                                                                        >  7:189225/1‑136 (MQ=255)
                                                          aTCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCCCCCCCGCGAGCGGGGGGGGCCCGGCGCCCGGACATTTTGTGGCGGCCAGCTGCCCGCTCCGccc                                                                        >  3:79532/1‑136 (MQ=255)
                                                             aaCTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCGCGCGCTGGGCGTGCCGGGCGCCCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCcgcgg                                                                     >  8:39836/1‑136 (MQ=255)
                                                             aaCTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGGCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGt                                                                     >  6:163824/1‑137 (MQ=255)
                                                              aCTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCTCGAGCTGGGCGGGGCCGGCGACCGTACTTTGTGTTGCGGCCTGCAGCCCGCAGCGCcgcggt                                                                    >  4:55955/1‑135 (MQ=255)
                                                              aCTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCTCGAGCGGGGCGTGCCGGTCGCCCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGtt                                                                    >  6:62249/1‑137 (MQ=255)
                                                              aCTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCCCGCGAGCGGGGCGTGGCGGGCGCCCGGACGTTGTGTGGCGGCCTGCTGGCCGCTCAGCcgcggg                                                                    >  8:18203/1‑135 (MQ=255)
                                                              aCTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGCGCGTGCCGGTCGCCCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGtt                                                                    >  4:352585/1‑137 (MQ=255)
                                                              aCTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCCCCCGCACGCGCGGGGCGTGGCGGTCGCCCGGACTTTGTTTTGCCGCCGGGAGGCAGCTCAGCcgcggt                                                                    >  8:6090/1‑135 (MQ=255)
                                                              aCTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCCCCAGCTCGAGCTGGGCGTGCCGGGCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCCGCCGCGtt                                                                    >  6:307113/1‑137 (MQ=255)
                                                                     gAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCTCGCGCTGGGCGGGGCGGGGGGCCGGGCGTTGTGTGGGGGGCGGGTGCCCCCTCAGCCGCGGTGGTgggg                                                             >  3:265973/1‑137 (MQ=255)
                                                                     gAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCTCGAGCTGGGCGTGGCGGTCGACCGTACGTTGTGTGGCGGGCTGCTGGCCGCGCAGCCGCGGTGGGCggg                                                             >  8:195983/1‑137 (MQ=255)
                                                                     gAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCGCGAGCGGGGCGGGGCGGGGGCCCGGGCTTTGTGTGGGGGGCGGCTGCCCGCGCCGCCGCGGTggggggg                                                             >  7:15558/1‑132 (MQ=255)
                                                                     gAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCCCTCGCTCTCGGCGTGCCGGTCGACCGAACTTTTTGTGGCGGCCTGCTGCCAGCACAGCCGCGTGGGTCggg                                                             >  7:306299/1‑137 (MQ=255)
                                                                          cgacgaGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCCCCTCGTGGCCCGCCAGCTCGAGGTGGGCGTGCCGGTCGTCCGTACTGTGGGTGGCGGCCGGCTGCCAGCGCAGCCGGGTGGGTGGGGGGTgg                                                        >  4:220234/1‑136 (MQ=255)
                                                                            actagcagcTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCcg                                                      <  7:213555/134‑1 (MQ=255)
                                                                            acgaGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCcg                                                      <  7:195983/137‑1 (MQ=255)
                                                                               agcagcTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCCCGCGCGCTGGGGGTGCCGGGCGGCCGGACTTTTTGTTGCGGGCGGGTGCCAGCTCACGCGCGGAGGTGGGGGGTTCggcgc                                                   >  8:213555/1‑136 (MQ=255)
                                                                                    cTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCGCTCGAGCTGGGCGGGCCGGGCGACCGTACGTTGTGTGGCGGCCTGCTGCCAGCTCCGCCGCGGTGGTCGGGGGTGCCGcggtcgat                                              >  7:176557/1‑137 (MQ=255)
                                                                                    cTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCCCCTCGCGCGGGGGGGGCCGGGCGCCCGGACTTTTTGTGGCGGGCTGCTGCCCGCTCCGCCGCGGGGGGGGGGGGTGGcgcgcgcgcg                                              >  4:134555/1‑131 (MQ=255)
                                                                                    cTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCACCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGGGGGGGGTGGCGcgatcgat                                              >  6:323417/1‑137 (MQ=255)
                                                                                             gtCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGa                                     <  5:94591/136‑1 (MQ=255)
                                                                                              tCGCCTGCCAGGAAAGCCGCGTCCACCTCGGGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGAt                                    <  4:19812/137‑1 (MQ=255)
                                                                                                gCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATgg                                  <  3:318510/137‑1 (MQ=255)
                                                                                                gCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATgg                                  <  3:55955/137‑1 (MQ=255)
                                                                                                gCCTGCCAGGAAAGCCGCGGCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATgg                                  <  1:184192/137‑1 (MQ=255)
                                                                                                gCCTGCCAGGAAAGCCGCGGCCACCTCGTGGCCCGCCAGCGCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGATATATATCTACCGCGATgg                                  <  5:323417/137‑1 (MQ=255)
                                                                                                     ccAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACTGCGATGGTCGcc                             <  5:252893/137‑1 (MQ=255)
                                                                                                     ccAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGcc                             <  4:47993/137‑1 (MQ=255)
                                                                                                     ccAGGAAAGCCGCGTACACCTCGTGGCCCGCGAGCTCGAGGTGGGCGTGCCGGTCTACCGTACTTTGTGTTGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGTTGACGCGATCGATCTACCGCGATGGTAGcc                             <  5:163131/137‑1 (MQ=255)
                                                                                                               cgcgTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCt                   <  4:121388/137‑1 (MQ=255)
                                                                                                               cgcgTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGATATTTTCGCCTTTGTGAGCt                   <  5:336643/137‑1 (MQ=255)
                                                                                                                  gTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCtgtg                <  2:23505/137‑1 (MQ=255)
                                                                                                                      aCCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCTgtgccgt            <  5:161901/137‑1 (MQ=255)
                                                                                                                      aCCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCTgtgccgt            <  4:54044/137‑1 (MQ=255)
                                                                                                                           gTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCTGTGCCGTGCCAt       <  6:179377/137‑1 (MQ=255)
                                                                                                                             ggCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCTGTGCCGTGCCATcc     <  5:98285/137‑1 (MQ=255)
                                                                                                                               cccGCCAGCTCGCGCTGGGCGTGCCGGTCGCCCGTACTTTTTGTGGCGGCCTGCTGCCAGCTCATCCGCGTTGGTCTGGGGGTCCGCGATCGATATACAGCGCTGGTCGCCTTTGTTAGCTGTGCCGTTCCATCCta   >  6:179262/1‑136 (MQ=255)
                                                                                                                                ccGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCTGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCTGTGCCGTGCCATCCTCg  <  8:308306/137‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                   
TTGCGCCCCCCGTTTCGAGTGCTCCAAATGCCGCGCCCTGCCTGGTCCCTGTATGCCTATCAACTGATCGAGCCCGACGAGCAGCTCGACCTGTTCGCCTGCCAGGAAAGCCGCGTCCACCTCGTGGCCCGCCAGCTCGAGCTGGGCGTGCCGGTCGACCGTACTTTGTGTGGCGGCCTGCTGCCAGCTCAGCCGCGTTGGTCGGGGGTGCCGCGATCGATCTACCGCGATGGTCGCCTTTGTGAGCTGTGCCGTGCCATCCTCG  >  ACB122/2641710‑2641974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑