Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,835,1930CA31.5% 94.4 / 12.4 54L162F (TTG→TTT) CJ019_02688hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (15/22);  new base A (17/0);  total (32/22)
Fisher's exact test for biased strand distribution p-value = 1.38e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGACTTCGCTTTGTAGCAGAAGGATCTGCCTGAGACCCAGTGCCTCACCGGAA  >  ACB122/2835058‑2835328
                                                                                                                                       |                                                                                                                                       
tgaagccAAGCATCGTAAGCTCCGCTAGATGTCGAGGGTTGTTGCTAGTCACTGATGATAGGGCATAGGGAGTCATAGTTTTACCTTATCAAAAAAATAGACCGGTCGTCTACTTTCTGTTTTGGATAATCTATACa                                                                                                                                        <  5:361106/133‑1 (MQ=255)
ggccgccAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACa                                                                                                                                        <  8:96215/136‑1 (MQ=255)
  ccgccAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAAc                                                                                                                                      <  5:137887/137‑1 (MQ=255)
                        cgcgATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATg                                                                                                                <  8:246490/137‑1 (MQ=255)
                          cgATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGaa                                                                                                              >  3:355900/1‑137 (MQ=255)
                          cgATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGGTGTGGATAATCTATAAAACGCTCTTTCGATGGTTTCTAATgta                                                                                                              >  2:75540/1‑135 (MQ=255)
                          cgATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTAGGTTGTGGTTAATATATTAAACGCTGTTTAGATTGTTTATAATTaa                                                                                                              >  7:91331/1‑137 (MQ=255)
                          cgATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACGTTCGGTTGTGGATAAACTATACAACGCTGTTTGGATGGTTGCTATTGaa                                                                                                              >  8:288901/1‑137 (MQ=255)
                          cgATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTAATTTAGGTTGTGGTTAATATATTAAACGCTGTTTAGATTGTTGGTAATGat                                                                                                              >  1:310224/1‑136 (MQ=255)
                          cgATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCGTCTTTCGGTTGTGGATTATATATAAAACGCTGTTTCGCTGGTTGCTAATTaa                                                                                                              >  2:33565/1‑137 (MQ=255)
                                cGAGGGTTGTTGCTAGGCACTGATGATATGGCATCGGGAGTCATAGGTTTACCGTATCTAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTTATGAAgcagca                                                                                                        <  4:67941/137‑1 (MQ=255)
                                          tgCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTGTACTTTGGGTTTTGGATAATATATAAAACGCTGTTTATATGGTTGATAATTAATCAGCAGAAGCTTCaa                                                                                              >  3:116362/1‑136 (MQ=255)
                                          tgCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTATCAGTTGTGGTTAAAATATAAAACGCTGTTTAGATGGTTTCTAAAGAAGAAGCAGAAAcatcat                                                                                              >  1:235251/1‑137 (MQ=255)
                                          tgCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCGACTTTCGGGTGTGGATAAACTATACAACGCTGTTTCTATGTTTGCTAATGAAGACTCAGCAGCTTCAt                                                                                              >  3:335869/1‑137 (MQ=255)
                                           gCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCGCAGTTAGGGTGTTGATAAAATATAAAAAGCTGTTTAGATGGTTTATAAAGAAGAAGCATCAGCttaaat                                                                                             >  2:343715/1‑133 (MQ=255)
                                            cTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCCGTTGTTGTTAATCTATAAAACTCTGTTTCGATGGTTGCTAATTAAGCAGCACAAGCTTCAttt                                                                                            >  6:489/1‑136 (MQ=255)
                                            cTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCGACTTTCGGTTGTGGATTATATATAAAACGCTGTTTCGATGGTTGATAATGAAGCAGCAGAAGCTTCAttt                                                                                            >  6:212959/1‑136 (MQ=255)
                                             tAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTgg                                                                                           <  4:355900/137‑1 (MQ=255)
                                                 cACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAgc                                                                                       >  8:43472/1‑137 (MQ=255)
                                                 cACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAgc                                                                                       <  7:43472/137‑1 (MQ=255)
                                                       tgatAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTGGACTTTCGGTTGTGGATAATCTATACAACGCTGTTTAGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCg                                                                                 >  8:171989/1‑137 (MQ=255)
                                                         atAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCGACTTTAGGGTGTGGTTAATATATAAAACGCTGTTTCGATTGTTGCTAATGAATCAGCAGGAGCTTCATTGGGAGCGCATCGAc                                                                               >  6:200778/1‑137 (MQ=255)
                                                          tAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACt                                                                              <  3:234435/137‑1 (MQ=255)
                                                              gCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATAAAACGCTGTTTGGATGGTTGCTAATGAAGCAGCAGAAGCTTAATTGGCAGCGCATCGACTGATg                                                                          >  3:356885/1‑137 (MQ=255)
                                                              gCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGGTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATg                                                                          >  8:32665/1‑137 (MQ=255)
                                                                aTCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCa                                                                        <  5:293904/137‑1 (MQ=255)
                                                                aTCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCa                                                                        <  1:28279/137‑1 (MQ=255)
                                                                aTCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCa                                                                        <  7:373171/137‑1 (MQ=255)
                                                                aTCGGGAGGCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCa                                                                        <  6:277587/137‑1 (MQ=255)
                                                                 tCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTGTACTTTAGGTTGTGGATAATCTATACAACGCTGTTTCGATTGTTGCTAATGAAGAAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAg                                                                       >  8:5894/1‑137 (MQ=255)
                                                                 tCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTGGACTTTGGGTTGTGGTTAATATATAAAAAGCTGTTTAGATGGTTTCTAATGAAGCAGCAGAAGCTTAATTTGCAGCGCATCGACTGATGCAg                                                                       >  1:88436/1‑137 (MQ=255)
                                                                          aTAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGtt                                                              >  8:194964/1‑137 (MQ=255)
                                                                           tAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGGTGTGGATAATATATAAAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATAGACTGATGCAGCCAAGCGTTa                                                             >  8:354983/1‑137 (MQ=255)
                                                                                    cGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGa                                                    <  1:33565/137‑1 (MQ=255)
                                                                                    cGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGa                                                    <  6:258381/137‑1 (MQ=255)
                                                                                     gTATCAAAAAAATAGACCGGTCGTCTTCATTGGCGTGTTGTTAATCTATACAACCGTCTATGGAATGTTGCTAATTGAGCAGCAGAAGCTTCATAGGGAGCGCATAGACAGAAGCAGGCAACCGGATCCAcgccgtc                                                   >  4:169797/1‑133 (MQ=255)
                                                                                     gTATCAAAAAAATAGACCGGTCGTCTAATTTAGTGTGTGGATAATATATAAAAACCTGTTTAGATTGTTTATAATGAATCAGCAGAATCTTAATTGGAAGCGACTACCCTGATGCCGACCACCGTTGCCCCGCggca                                                   >  6:254028/1‑135 (MQ=255)
                                                                                        tCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGACTTc                                                <  4:160979/137‑1 (MQ=255)
                                                                                         cAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATAAAACGCTGTTTCGATGGTTTCTAATTAATCAGCAGAAGCTTCAATGGCAGCGCATCGACTGATGAAGCCAAGCCTTACCAACCGGACTTca                                               >  8:322031/1‑136 (MQ=255)
                                                                                           aaaaaaTAGACCGGTCGTGTACTTTAGGTTGTGGATAATCTATAAAACGCTGTTTAGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTTGCAGCGCATAGACTGATGCCGCCAAGCGGTACCAAGCGGAcgtggcg                                             >  8:72170/1‑131 (MQ=255)
                                                                                           aaaaaaTAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGACTTCGCt                                             <  6:286947/137‑1 (MQ=255)
                                                                                                       ggTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGACTTCGCTTTGTAGCAGAAg                                 <  5:212959/137‑1 (MQ=255)
                                                                                                           gtgtACTTTCGGTTGTGGATAATCTATAAAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTTGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGACTTCGCTTTGTAGCAGAAGGATc                             >  6:123410/4‑137 (MQ=255)
                                                                                                               aCTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGACTTCGCTTTGTAGCAGAAGGATCTGcc                         <  4:169840/137‑1 (MQ=255)
                                                                                                                 tttCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGACTTCGCTTTGTAGCAGAAGGATCTGCCTg                       <  8:91331/137‑1 (MQ=255)
                                                                                                                        tgtgGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGAGCATCGACTGATGCAGCCAAGCGTTACCACGCGGACTTCGCTTTGTAGCAGAAGGATCTGCCTGAGACCCa                >  2:290231/1‑137 (MQ=255)
                                                                                                                         gtgGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGACTTCGCTTTGTAGCAGAAGGATCTGCCTGAGACCCAg               <  2:88436/137‑1 (MQ=255)
                                                                                                                         gtgGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGACTTCGCTTTGTAGCAGAAAGATCTGCCTGAGACCCAg               >  3:192769/1‑137 (MQ=255)
                                                                                                                           ggATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGACTTCGCTTTGTAGCAGAAGGATCTGCCTGAGACCCAGTg             <  8:239592/137‑1 (MQ=255)
                                                                                                                                    ataCAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGACTTCGCTTTGTAGCAGAAGGATCTGCCTGAGACCCAGTGCCTCACCgg    >  8:151891/1‑137 (MQ=255)
                                                                                                                                    ataCAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGACTTCGCTTTGTAGCAGAAGGATCTGCCTGAGACCCAGTGCCTCACCgg    >  6:4923/1‑137 (MQ=255)
                                                                                                                                    ataCAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGACTTCGCTTTGTAGCAGAAGGATCTGCCTGAGACCCAGTGCCTCACCgg    >  4:74866/1‑137 (MQ=255)
                                                                                                                                     taCAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGACTTCGCTTTGTAGCAGAAGGATCTGCCTGAGACCCAGTGCCTCACCGGa   >  1:177044/1‑137 (MQ=255)
                                                                                                                                      aCAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGACTTCGCTTTGTAGCAGAAGGATCTGCCTGAGACCCAGTGCCTCACCGGaa  <  3:198915/137‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
TGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAGGTTTACCGTATCAAAAAAATAGACCGGTCGTCTACTTTCGGTTGTGGATAATCTATACAACGCTGTTTCGATGGTTGCTAATGAAGCAGCAGAAGCTTCATTGGCAGCGCATCGACTGATGCAGCCAAGCGTTACCACGCGGACTTCGCTTTGTAGCAGAAGGATCTGCCTGAGACCCAGTGCCTCACCGGAA  >  ACB122/2835058‑2835328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑